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| AUTOMATED DNA SEQUENCING AND ANALYSIS
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Edited By
Mark Adams, Director, EST Laboratory
Chris Fields, Director, Department of Genome Informatics
J. Venter, The Institute for Genomic Research, Gaithersburg, Maryland, U.S.A.
Description
A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of
automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It
presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted.
By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied
with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small
laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and
genomic sequencing projects.
Audience
Reference text for researchers and technicians using automated DNA sequencers for studies in molecular genetics, genome sciences, and cell biology.
Contents
Sequencing Instruments and Strategies:
E. Chen, The Efficiency of Automated DNA Sequencing.G.M. Church,
G. Gryan, N. Lakey, S. Kieffer-Higgins, L. Mintz, M. Temple, M. Rubenfield, L. Jaehn, H. Ghazizadel, K. Robison, and P. Richterick,
Automated Multiplex Sequencing.X.C. Huang and R.A. Mathies, Application of Capillary Array Electrophoresis to DNA Sequencing.
R. Drmanac, S. Drmanac, J. Jarvis, and I. Labat, Sequencing by Hybridization.A. Martin-Gallardo, J. Lamerdin, and A. Carrano,
Shotgun Sequencing.A. Bodenteich, S. Chissoe, Y.-F. Wang, and B. Roe, Shotgun Cloning as the Strategy of Choice to Generate
Templates for High Throughput Dideoxynucleotide Sequencing.C.M. Berg, G. Wang, K. Isono, H. Kasai, and D.E. Berg, Transposon-Facilitated
Large-Scale DNA Sequencing.C.H. Martin, C.A. Mayeda, C.A. Davis, M.P. Strathmann, and M. Palazzolo, Transposon-Facilitated
Sequencing: An Effective Set of Procedures to Sequence DNA Fragments Smaller than 4kb.L. Liu and R.D. Fleischmann, Construction
of Exonuclease III Nested Deletion Sets for Rapid DNA Sequencing.M. Adams, Expressed Sequence Tags as Tools for Physiology
and Genomics.W.R. McCombie, The Use of Automated DNA Sequencing in the Analysis of cDNAs of Model Organisms.
Sample
Preparation and Sequencing Methods: Libraries:
R.L. Stalligs, N.A. Doggett, A. Ford, J. Longmire, C.E. Hildebrand, L.L.
Deaven, and R. Moyzis, Applications of Cosmid Libraries in Genome Mapping and Sequencing Efforts.D.A. Smoller, W.J. Kimmerly,
O. Hubbard, C. Ericsson, C.H. Martin, and M.J. Palazzolo, A Role for the P1 Cloning System in Genome Analysis.N.A. Doggett,
D.L. Grady, J.L. Longmire, and L.L. Deaven, Generation and Mapping of Chromosome Specific Sequence-Tagged Sites (STS).R. Moreno
and R. Fuldner, Construction of cDNA Libraries.M.B. Soares, Construction of Directionally Cloned cDNA Libraries and Phagemid
Vectors.A. Swaroop, Construction of Directional cDNA Libraries.J.M. Kwak and J.G. Nam, Preparation of cDNA Libraries
from Brassica.J.M. Sikela, T.J. Stevens, J.A. Hopkins, A.S. Wilcox, J. Glod, A.S. Khan, and A.K. Orpana, Abundance Screening
of Human cDNA Libraries.G. Lennon, High Density Grid Technologies.
Automated Sample Preparation:
D.R.
Sibson, Solid Phrase Preparation of Sequencing Templates from PCR Products.A. Holmberg, G. Fry, and M. Uhlon, Automatic
Preparation of DNA Templatesfor Sequencing on the ABI Catalyst Robotic Workstation.T. Hawkins, Custom Magnetic Particles: Their
Use in DNA Purification.
Sequencing Methods:
W.R. McCombie and A. Martin-Gallardo, Large-Scale Automated Sequencing
of Human Chromosomal Regions.L. Rowen and B.F. Koop, Zen and the Art of Large-Scale Genomic Sequencing.J.M. Kelley,
Automated Dye Terminator DNA Sequencing.D.M. Muzny, S. Richards, Y. Shen, and R.A. Gibbs, PCR Based Strategies for Gap Closure
in Large Scale Sequencing Projects.S. Richards, D.M. Muzny, A.B. Civitello, F. Lu, and R.A. Gibbs, Sequence Map Gaps and Directed
Reverse Sequencing for the Completion of Large Sequencing Projects.F. Iris, Optimized Methods for Large-Scale Shotgun DNASequencing
in Alu-Rich Genomic Regions.S.G. Burgett and P.R. Rosteck, Jr., Use of Dimethylsulfoxide to Improve Fluorescent, Tag Cycle
Sequencing.
Informatics: Sequence Assembly Theory and Algorithms:
C. Tibbetts, J.M. Bowling, and J.B. Golden, III,
Neural Networks for Automated Base Calling of Gel Based DNA Sequencing Ladders.G. Myers, Advances in Sequence Assembly.S.
Honda, N.W. Parrott, and C.B. Lawrence, Computer Aided Sequence Reconstruction: Software Support for Multiple Large-Scale Sequencing
Strategies.C. Burks, M.L. Engle, S. Forrest, R.J. Parsons, C.A. Soderlund, and P.E. Stolorz, Relaxation and Optimization Methods
for Sequence Assembly.
Data Analysis Tools:
G. Sutton and T. Kerlavage, Software Tools for Protein Similarity
Searching.J.M. Claverie, Large-Scale Sequence Analysis.J. Shavlik, Finding Frame Shift Errors in Anonymous DNA.B.
Rappaport, J. Gatewood, C. Fields, and N. Doggett, Integrating Repeat Identification withThermal Calculations.J. Jurka,
Approaches to Identification and Analysis of Interspersed Repetitive DNA Sequences.O. White and T. Dunning, Compositional Properties
of Exons and Introns.E.C. Uberbacher, X. Guan, and R.J. Mural, A Practical Guide to the GRAIL Email Server.S. Henikoff,
J. Henikoff, S. Agus, and J.C. Wallace, Searching for Homologies to Protein Blocks by Electronic Mail.C. Fields, Integrating
Computational and Experimental Methods.
Data Management and Databases:
S. Lewis, Design Issues in Developing
Laboratory Information Management Systems.J. Cuticchia, A Relational Database Primer for Molecular Biologists.J.M. Cherry
and S.W. Cartinhour, ACEDB: A Tool for Biological Information.R. Overbeek and M. Price, The Integration of Curated Biological
Databases.
| Bibliographic details |
Hardbound, 368 pages, publication date: MAY-1994
ISBN-13: 978-0-12-717010-7
ISBN-10: 0-12-717010-3
Imprint: ACADEMIC PRESS
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