Research Needs To order this title, and for more information, click here
By Gilbert S. Omenn Albert H. Teich
Description Proceedings from an EPA workshop on research needs involving biotechnology.
Audience
Environmental engineers and scientists.
Contents Part I Workshop Summary
Worshop Summary
Gilbert S. Omenn and Albert H. Teich
Introduction and Background
Key Concerns and Recommendations
Environmental Effects
Health Effects
Monitoring and Quality Assurance
Control Technologies
Part II Papers
Research Plan for Test
Methods Development For Risk Assessment of Novel Microbes Released Into Terrestrial and Aquatic Ecosystems
Al Bourquin and Ramon Seidler
I. Introduction
A. Goal
B. Risk Assessment
C. Two Major Approaches
1. Data Base Development
2. Research
D. Short and Long
Term Needs
E. Relevance to EPA Needs
II. Novel Organisms
III. Development of Test Methods for the Detection, Identification, and Enumeration
of Novel Organisms
A. Statement of Research Problems
B. Availability of Data Base
C. Approaches
1. Conventional Techniques
2. Molecular
Techniques
D. Short Term Products
E. Long Term Products
IV. Developments of Test Methods for Assessing Fate of Novel Organisms
A. Statement
of Research Problems
B. Availability of Data Base
C. Approaches
1. The Microcosm Approach
2. Rationale for Selecting Ecosystems
a. Terrestrial
Research
b. Aquatic Research
D. Short Term Products
E. Long Term Products
V. Development of Test Methods for Assessing Genetic Stability
of Novel Organisms
A. Statement of Research Problems
B. Availability of Data Base
C. Approaches
1. Naked Plasmid DNA in Novel Organisms
2. Stability of Plasmid DNA in Novel Organisms
3. Approaches
D. Short Term Products
E. Long Term Products
VI. Development of Test Methods
for Assessing Hazards of Released Novel Organisms
A. Statement of Research Problems
B. Availability of Data Base
C. Approaches
D. Short
Term Products
E. Long Term Products
VII. Summary
VIII. Acknowledgements
IX. Literature Cited
Biotechnology Health Assessment Research
Plan
Marvin Rogul and John R. Fowle III
I. Introduction
II. Health Effects Work Group Panel Recommendations
A. Data Gathering and
Information Management
B. Selection of Organisms for Validating Subpart M Test Approach
C. Protocol Development for Infectivity, Pathogenicity,
and Metabolic Characteristics of Recombinant Microorganisms
D. Bacterial Pathogenicity Categories
E. Establishment and Management of
a Data Base of Characteristics of the Potential Hazards of Genetically Modified Materials
F. Selection and Assessment of Safe Hosts
G.
Development of Molecular Probes
III. Discussion
A. Risk Assessment
B. Foundation Laid by the NIH Recombinant DNA Advisory Committee
1.
E. coli Studies Which Influenced the Development of the RAC Guidelines
2. Experiments Simulating High Risk Conditions, Promoting and
Detecting Genetic Interchange
IV. References
Environmental Engineering Research Support Proposal
John Burckle and Albert D. Venosa
I. Legislation
II. Regulatory Needs
III. Overall Program Approach
IV. Summary of Proposed Environmental Engineering Efforts Related to
Regulatory Needs
A. Regulatory Needs
B. Program Structure
C. Proposed Approach
D. Development of Engineering Information and Methodology
for Risk Assessment, Reduction and Management for Genetically Engineered Microorganisms in Biologically Based Manufacturing Processes
and Deliberate Environmental Release
1. Accidental and Deliberate Release from Biologically Based Manufacturing Processes
2. Deliberate
Release Into the Environment
Monitoring Techniques For Genetically Engineered Microorganisms
David Glaser, Tim Keith, Peg Riley, Geoff
Chambers, John Manning, Susan Hattingh and Ralph Evans
I. Introduction
II. Sampling Considerations
A. Introduction
B. Qualitative Sampling
C. Desorption from Sediments
D. Enrichment
E. Partitioning in the Environment
F. Issues in Sampling Methods
III. Monitoring Techniques
A. Conventional Microbiological Techniques
B. Immunological Techniques
1. Standard Methods of Antibody Production
2. Monoclonal Methods
of Antibody Production
C. The Use of Genetic Markers
D. Molecular Techniques
1. Restriction Enzyme Mapping
2. DNA Probes
3. DNA-DNA Hybridization
4. Genomic Sequencing
IV. Microcosm Test for Monitoring Techniques
A. Microcosm Construction
B. Microcosm Methodology
C. Sample Protocol
D. Containment
E. Points to Consider
V. Quality Assurance
A. Introduction
B. Testing for Sensitivity and Specificity
C. Testing for Linkage
Between Markers and rDNA
D. Summary
VI. Conclusion
A. Monitoring Techniques
B. Scenarios for Protocol Development
C. Research Needs
VII.
Literature Cited
Appendix: List of Participants
Bibliographic details
Hardbound, 179 pages, publication date: DEC-1986
ISBN-13: 978-0-8155-1105-2
ISBN-10: 0-8155-1105-1
Imprint: WILLIAM ANDREW
Price and Ordering
Price: GBP 85.99 USD 142 EUR 100.95
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