 |
|
| ADVANCED BACTERIAL GENETICS: USE OF TRANSPOSONS AND PHAGE FOR GENOMIC ENGINEERING, 421
| |
| | |
|
|
To order this title, and for more information, click here
Kelly Hughes, Department of Biology, University of Utah, Salt Lake City, U.S.A.
Included in series
Methods in Enzymology,
Description
The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly
respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised
by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant
today?truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the
use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons;
and phage.
Audience
Biochemists, geneticists, and molecular biologists.
Contents
Section I. Strain Collections and Genetic Nomenclature.
Chapter 1. Strain Collections and Genetic Nomenclature.
Section II. Transposons.
Chapter 2: Use of Antibiotic Resistant Transposons for Mutagenesis.
Chapter 3: In vivo Mutagenesis Using EZ-Tn5TM.
Chapter 4: Identification
of essential genes in bacteria.
Chapter 5: Isolation and use of linked transposons.
Chapter 6: Localized mutagenesis.
Chapter 7: Generation
of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons.
Chapter 8:
Target-Directed Proteolysis in vivo.
Chapter 9: Sets of transposon generated sequence-tagged mutants for structure-function analysis
and engineering.
Chapter 10: Using Genomic Microarrays to Study Insertional/Transposon Mutant Libraries.
Chapter 11: Screening Transposon
Mutant Libraries Using Full-Genome Oligonucleotide Microarrays.
Chapter 12: Creating recombination-activated genes and sequence-defined
mutant libraries using transposons
Chapter 13: Use of transposons to study genetic regulation fusions.
Chapter 14: Genomic screening
for regulatory genes using the T-POP transposon.
Section III. Phage.
Chapter 15: Recombineering: in vivo genetic engineering in E. coli,
S. enterica and beyond.
Chapter 16: – -Red Genetic Engineering in Salmonella enterica serovar Typhimurium.
Chapter 17: Integrase.
Chapter
18: Challenge phage.
Chapter 19: MudP22.
Chapter 20: Phage metagenomics.
| Bibliographic details |
Hardbound, 320 pages, publication date: FEB-2007
ISBN-13: 978-0-12-373749-6
ISBN-10: 0-12-373749-4
Imprint: ACADEMIC PRESS
|
| Price and Ordering |
Price:
GBP 105.99
USD 175
EUR 124.95
|  |
Books and book related electronic products are priced in US dollars (USD), euro (EUR), and Great Britain Pounds (GBP). USD prices apply to the Americas and Asia Pacific. EUR prices apply in Europe and the Middle East. GBP prices apply to the UK and all other countries.
|
See also information about conditions of sale & ordering procedures, and links to our regional sales offices.
|
092/900
Last update: 25 Nov 2009
|
|
| |
| | |
|
|
| |
Home | Elsevier Sites | Privacy Policy | Terms and Conditions | Feedback | Site Map | A Reed Elsevier Company