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Edited By J. McIntosh
Description Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly
in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value
it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging
in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written
by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each
section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists
believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules,
helping us toward the goal of an atomic resolution understanding of living systems.
Audience
Researchers in various fields of biology who utilize electron microscopy.
Contents Introduction: McIntosh - A new era in electron microscopy of cells
Preparative Methods for Cellular Electron Microscopy
1. The physics of rapid cooling and its implications for cryo-immobilization of cells
2. Cryopreparation Methods for Electron Microscopy
of Selected Model Systems
3. Cryo-preparation methodology for plant cell biology
4. Correlative Light and Electron Microscopy of Early
C. elegans Embryos in Mitosis
Imaging Fixed Cells in Three Dimensions
5. Understanding microtubule organizing centers by comparing
mutant and wild-type structures with electron tomography
6. Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron
Tomography
7. Electron Microscopy of archaea
8. Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography
9. Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis
10. Electron Tomographic Methods for Studying the Chemical
Synapse
11. Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
12. EM
Analysis of the Leading Edge in Migrating Cells
13. Imaging actomyosin in situ
Imaging Frozen-Hydrated Cells and Cell Parts
14. Electron tomography of bacterial chemotaxis receptor assemblies
15. How to ?read? a vitreous section
16. Single Particle Electron
Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction
17. Electron microscopy of microtubule-based cytoskeletal
machinery
18. Reconstructing the endocytotic machinery
Localizing Macromolecules in Cells
19. 3-D Immuno-localization
with plastic sections
20. EM analysis of viral morphogenesis
21. Electron tomography of immuno-labeled cryosections
22. Visualizing
macromolecules with fluoronanogold: from photon microscopy to electron tomography
23. Markers for correlated light and electron microscopy
24. Localizing Specific Elements Bound to Macromolecules by EFTEM
25. Localization of protein complexes by pattern recognition
Aspects of Data Collection and Analysis Instrumentation for image acquisition
26. Application of Energy Filtered
Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples
27. Optimization of Image Collection for Cellular
Electron Microscopy
28. Future Directions for Camera Systems in Electron Microscopy
Computational methods for the analysis
of cellular data
29. Structure determination in situ by averaging of tomograms
30. Methods for image segmentation in cellular
tomography
31. Database Resources for Cellular Electron Microscopy
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