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To order this title, and for more information, click here
Irina Klimanskaya, Advance Cell Technology, Worcester, MA, USA
Robert Lanza, Chief Scientific Officer, Advanced Cell Technology, MA, USA; Adjunct Professor, Institute of Regenerative Medicine, Wake Forest University
School of Medicine, NC, USA Robert Lanza Advanced Cell Technology 381 Plantation Street Worcester, MA 01605
Description
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume
is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world.
The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with
great attention to detail. The chapters provide a brief review of each field followed by a ?cookbook? and handy illustrations. The collection
of protocols includes the isolation and maintenance of stem cells from various species using ?conventional? and novel methods, such as
derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of
somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find
that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols
and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and
troubleshooting of these state-of-the-art technologies in other laboratories.
Audience
Biochemists, cell and molecular biologists, and bioengineers working with stem cells.
Contents
I. Ectoderm
Isolation and characterization of neural stem cells.
Neural stem cells and their manipulation.
Retinal stem cells.
Epithelial
Skin Stem Cells.
Dental pulp stem cells.
Section II. Mesoderm
Postnatal skeletal stem cells.
Hematopoietic stem cells.
Hemangioplast
and its progeny.
Kidney Epithelial Cells.
Ovarian germ cells.
Spermatogonial Stem Cells.
Section III. Endoderm
Stem cells in the lung.
Pancreatic cells and their progenitors.
Intestinal epithelial stem cells and their progenitors.
IV. Extraembryonic and perinatal stem
cells
Trophoblast stem cells.
Pluripotent Stem Cells from Germ Cells.
Amniotic Fluid and placenta stem cells.
Cord blood progenitor cells.
| Bibliographic details |
Hardbound, 576 pages, publication date: NOV-2006
ISBN-13: 978-0-12-373650-5
ISBN-10: 0-12-373650-1
Imprint: ACADEMIC PRESS
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| Price and Ordering |
Price:
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USD 175
EUR 124.95
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Last update: 3 Oct 2009
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