Guide for Authors

  • All journal information and instructions compiled in one document (PDF) in just one mouse-click Author information pack

    Introduction
    • Types of paper
    • Contact details for submissions
    • Page charges
    BEFORE YOU BEGIN
    • Ethics in publishing
    • Conflict of interest
    • Submission declaration and verification
    • Changes to authorship
    • Copyright
    • Role of the funding source
    • Funding body agreements and policies
    • Open access
    • Language (usage and editing services)
    • Informed consent and patient details
    • Submission
    • Referees
    • Guidelines for taxonomic papers (i.e new taxa descriptions)
    PREPARATION
    • Use of word processing software
    • Article structure
    • Essential title page information
    • Abstract
    • Graphical abstract
    • Highlights
    • Keywords
    • Chemical compounds
    • Abbreviations
    • Acknowledgements
    • Units
    • Nucleotide sequence data
    • Math formulae
    • Footnotes
    • Artwork
    • Illustration services
    • Tables
    • References
    • Video data
    • Supplementary data
    • Data at PANGAEA
    • Data deposit and linking
    • Google Maps and KML files
    • Mol files
    • Interactive Phylogenetic Trees
    • Submission checklist
    AFTER ACCEPTANCE
    • Use of the Digital Object Identifier
    • Proofs
    • Offprints
    AUTHOR INQUIRIES



    Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
    Systematics: Theoretical and practical issues dealing with classification and taxonomy, i.e. (i) new descriptions or revisions of prokaryotic taxa, including descriptions of not-yet cultured taxa in the category Candidatus, (ii) innovative methods for the determination of taxonomical and genealogical relationships, (iii) evaluation of intra-taxon diversity through multidisciplinary approaches, (iv) identification methods.
    Applied Microbiology: all aspects of agricultural, industrial, and food microbiology are welcome, including water and wastewater treatment.
    Comparative biochemistry and genomics: studies concerning biochemical/metabolic and genomic diversity of cultured as well as yet-uncultured Bacteria and Archaea.
    Ecology: descriptions of the microbial diversity in natural and man-made ecosystems; studies quantifying the size, dynamics, and function of microbial populations; innovative research on the interaction of microorganisms with each other and their biotic and abiotic environment.

    Types of paper

    Minireviews are short review papers, normally not exceeding 6 printed pages, concerned with matters of particular interest and current importance. They must be based on published articles.
    Full-length papers should be as concise as possible.
    Short communications should not exceed 3 printed pages, including illustrations. The paper should be preceded by a brief summary, and division of the text into various sections may be dispensed with.

    Contact details for submissions

    For questions on the submission and reviewing process, please contact leeb@mikro.biologie.tu-muenchen.de. For technical questions, please use our help site at: http://epsupport.elsevier.com/. Here you will be able to learn more about the online submission and editorial system via interactive tutorials, explore a range of problem solutions via our knowledgebase, and find answers to frequently asked questions. You will also find our support contact details should you need any assistance from one of our customer service representatives.

    Page charges

    This journal has no page charges.

    Ethics in publishing

    For information on Ethics in publishing and Ethical guidelines for journal publication see http://www.elsevier.com/publishingethics and http://www.elsevier.com/journal-authors/ethics.

    Conflict of interest

    All authors must disclose any financial and personal relationships with other people or organizations that could inappropriately influence (bias) their work. Examples of potential conflicts of interest include employment, consultancies, stock ownership, honoraria, paid expert testimony, patent applications/registrations, and grants or other funding. See also http://www.elsevier.com/conflictsofinterest. Further information and an example of a Conflict of Interest form can be found at: http://help.elsevier.com/app/answers/detail/a_id/286/p/7923.

    Submission declaration and verification

    Submission of an article implies that the work described has not been published previously (except in the form of an abstract or as part of a published lecture or academic thesis or as an electronic preprint, see http://www.elsevier.com/postingpolicy), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder. To verify originality, your article may be checked by the originality detection service CrossCheck http://www.elsevier.com/editors/plagdetect.

    Changes to authorship

    This policy concerns the addition, deletion, or rearrangement of author names in the authorship of accepted manuscripts:
    Before the accepted manuscript is published in an online issue: Requests to add or remove an author, or to rearrange the author names, must be sent to the Journal Manager from the corresponding author of the accepted manuscript and must include: (a) the reason the name should be added or removed, or the author names rearranged and (b) written confirmation (e-mail, fax, letter) from all authors that they agree with the addition, removal or rearrangement. In the case of addition or removal of authors, this includes confirmation from the author being added or removed. Requests that are not sent by the corresponding author will be forwarded by the Journal Manager to the corresponding author, who must follow the procedure as described above. Note that: (1) Journal Managers will inform the Journal Editors of any such requests and (2) publication of the accepted manuscript in an online issue is suspended until authorship has been agreed.
    After the accepted manuscript is published in an online issue: Any requests to add, delete, or rearrange author names in an article published in an online issue will follow the same policies as noted above and result in a corrigendum.

    Copyright

    This journal offers authors a choice in publishing their research: Open access and Subscription.

    For subscription articles
    Upon acceptance of an article, authors will be asked to complete a 'Journal Publishing Agreement' (for more information on this and copyright, see http://www.elsevier.com/copyright). An e-mail will be sent to the corresponding author confirming receipt of the manuscript together with a 'Journal Publishing Agreement' form or a link to the online version of this agreement.
    Subscribers may reproduce tables of contents or prepare lists of articles including abstracts for internal circulation within their institutions. Permission of the Publisher is required for resale or distribution outside the institution and for all other derivative works, including compilations and translations (please consult http://www.elsevier.com/permissions). If excerpts from other copyrighted works are included, the author(s) must obtain written permission from the copyright owners and credit the source(s) in the article. Elsevier has preprinted forms for use by authors in these cases: please consult http://www.elsevier.com/permissions.

    For open access articles
    Upon acceptance of an article, authors will be asked to complete an 'Exclusive License Agreement' (for more information see http://www.elsevier.com/OAauthoragreement). Permitted reuse of open access articles is determined by the author's choice of user license (see http://www.elsevier.com/openaccesslicenses).

    Retained author rights
    As an author you (or your employer or institution) retain certain rights. For more information on author rights for:
    Subscription articles please see http://www.elsevier.com/journal-authors/author-rights-and-responsibilities.
    Open access articles please see http://www.elsevier.com/OAauthoragreement.

    Role of the funding source

    You are requested to identify who provided financial support for the conduct of the research and/or preparation of the article and to briefly describe the role of the sponsor(s), if any, in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. If the funding source(s) had no such involvement then this should be stated.

    Funding body agreements and policies

    Elsevier has established agreements and developed policies to allow authors whose articles appear in journals published by Elsevier, to comply with potential manuscript archiving requirements as specified as conditions of their grant awards. To learn more about existing agreements and policies please visit http://www.elsevier.com/fundingbodies.

    Open access

    This journal offers authors a choice in publishing their research:

    Open access
    • Articles are freely available to both subscribers and the wider public with permitted reuse
    • An open access publication fee is payable by authors or their research funder
    Subscription
    • Articles are made available to subscribers as well as developing countries and patient groups through our access programs (http://www.elsevier.com/access)
    • No open access publication fee

    All articles published open access will be immediately and permanently free for everyone to read and download. Permitted reuse is defined by your choice of one of the following Creative Commons user licenses:
    Creative Commons Attribution (CC BY): lets others distribute and copy the article, to create extracts, abstracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation), to include in a collective work (such as an anthology), to text or data mine the article, even for commercial purposes, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article, and do not modify the article in such a way as to damage the author's honor or reputation.
    Creative Commons Attribution-NonCommercial-ShareAlike (CC BY-NC-SA): for non-commercial purposes, lets others distribute and copy the article, to create extracts, abstracts and other revised versions, adaptations or derivative works of or from an article (such as a translation), to include in a collective work (such as an anthology), to text and data mine the article, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article, do not modify the article in such a way as to damage the author's honor or reputation, and license their new adaptations or creations under identical terms (CC BY-NC-SA).
    Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND): for non-commercial purposes, lets others distribute and copy the article, and to include in a collective work (such as an anthology), as long as they credit the author(s) and provided they do not alter or modify the article.

    To provide open access, this journal has a publication fee which needs to be met by the authors or their research funders for each article published open access.
    Your publication choice will have no effect on the peer review process or acceptance of submitted articles.

    The open access publication fee for this journal is $USD 2500, excluding taxes. Learn more about Elsevier's pricing policy: http://www.elsevier.com/openaccesspricing.

    Language (usage and editing services)

    Please write your text in good English (American or British usage is accepted, but not a mixture of these). Authors who feel their English language manuscript may require editing to eliminate possible grammatical or spelling errors and to conform to correct scientific English may wish to use the English Language Editing service available from Elsevier's WebShop (http://webshop.elsevier.com/languageediting/) or visit our customer support site (http://support.elsevier.com) for more information.

    Informed consent and patient details

    Studies on patients or volunteers require ethics committee approval and informed consent, which should be documented in the paper. Appropriate consents, permissions and releases must be obtained where an author wishes to include case details or other personal information or images of patients and any other individuals in an Elsevier publication. Written consents must be retained by the author and copies of the consents or evidence that such consents have been obtained must be provided to Elsevier on request. For more information, please review the Elsevier Policy on the Use of Images or Personal Information of Patients or other Individuals, http://www.elsevier.com/patient-consent-policy. Unless you have written permission from the patient (or, where applicable, the next of kin), the personal details of any patient included in any part of the article and in any supplementary materials (including all illustrations and videos) must be removed before submission.

    Submission

    Submission to this journal proceeds totally online and you will be guided stepwise through the creation and uploading of your files. The system automatically converts source files to a single PDF file of the article, which is used in the peer-review process. Please note that even though manuscript source files are converted to PDF files at submission for the review process, these source files are needed for further processing after acceptance. All correspondence, including notification of the Editor's decision and requests for revision, takes place by e-mail removing the need for a paper trail.

    Submit your article
    Please submit your article via http://ees.elsevier.com/syapm.

    Referees

    Please submit the names and institutional e-mail addresses of several potential referees. For more details, visit our Support site. Note that the editor retains the sole right to decide whether or not the suggested reviewers are used.

    Guidelines for taxonomic papers (i.e new taxa descriptions)

    Submitted taxonomic manuscripts must fulfill the following standards:

    (i) For any new species of a genus a type strain must be designated, this strain must be deposited in two culture collections in different countries and the accession numbers must be available on the date of the submission. This strain must be comprehensively studied by means of a polyphasic approach, including genetic and phenotypic traits.

    (ii) It is highly encouraged that more than one single strain is used for descriptions of new genera and/or species. It is of the utmost importance that all strains are equally studied in order to describe intraspecific diversity. In addition, it is necessary to perform DNA-DNA hybridization analysis with the type strain of the species and those strains thought to belong to the same taxon. Alternatively, species identity may be demonstrated by a high degree of similarity in banding patterns after using genomic fingerprinting techniques, such as ERIC-, REP-, BOX-, (GTG)5- and/or RAPD-PCR, which in most cases may show clonality.

    (iii) Any taxonomic paper should be accompanied by a fingerprint of the strain/strains used to classify a new taxon by means of methods like RAPD, PFGE, or even whole cell protein profiles. It is obligatory to show that the different strains in use, belonging to the same taxon, are not clonal varieties. The picture of the profiles showing the differences among the isolates of the same taxon should be submitted as supplementary material if they do not provide additional information. In cases of clonality, the fact should be discussed in order to understand the reasons to use multiple identical clones (mainly justifiable if they had been isolated from different samples). Finally, single strain species descriptions should be accompanied by a fingerprint of the new isolate and the closest relative type strains.

    (iv) The nearly complete 16S rRNA gene sequence (more than 1300 nucleotides) of the type strain of the species must be studied, and deposited in a public repository. The accession number must be given.

    (v) The type strains of the most closely related taxa must be simultaneously investigated. It is of the utmost importance that all reference strains are tested with the same methods as the strains of the new taxon. Submissions where the discriminative tests of the reference strains are simply taken from the literature will not be reviewed, unless they are based on results obtained with the same methods. If possible, phenotypic tests should be performed by means of standardized methods.

    (vi) DNA-DNA hybridization experiments (DDH) must be carried out with the type strain of the new taxon and the closest relatives in any case where the 16S rRNA gene sequence similarities lie above 97%. Alternatively, the editors encourage authors to substitute DDH for average nucleotide identity (ANI) by means of complete pair wise genome comparisons, or by partially sequencing the genomes for about 20% of their length, as recommended in Richter and Rosselló-Móra (2009) PNAS 106 (45), 19126 – 19131.

    (vii) It is highly recommended that chemotaxonomic markers are studied. It will be compulsory to show the chemical composition peculiarities for new genera descriptions. Recommended markers are fatty acid profiles, polar lipid composition, quinone types, polyamine patterns, and peptidoglycan type/composition (in particular for the description of Gram-positive bacteria, but also considering that Gram-negative bacteria may differ in their diamino acid composition).

    (viii) It is compulsory to give the G+C mole % values of the genome of the type strain of the type species of a new genus.

    (ix) For other gene sequence analyses (e.g. MLSA), all accession numbers of the generated sequences must be given. In addition, it is compulsory to submit the full alignments used to generate the trees that are to be published. The alignments will be published as online supplementary material.

    (x) For any new taxon, the etymology of the proposed name must be provided.

    (xi) For any new taxon, a protologue indicating the discriminative traits that are characteristic of the taxon should be clearly given. One should avoid linking tables to the protologue. The text should contain all the necessary information that explains how the taxon can be identified.

    (xii) The discriminative phenotype should be summarized in a diagnostic table where the most closely related taxa are also indicated.

    Single strain descriptions. Single strain taxa descriptions are accepted only in exceptional cases. Exceptionalness may result from the relevance of the strain and of the work that has been done with it independently whether it has been published elsewhere. Cases such as full genome sequencing, ecological relevance in their environment, being a result of the isolation of a “candidatus” organism, or very especial metabolisms may be regarded as being of additional interest. Also, if the taxonomic work is done exhaustively and properly, adding new approaches, e.g. MLSA, ANI calculations, completing the phenotypic analyses by means of several chemotaxonomic markers and exhaustive metabolic studies, and any additional information that balances the lack of additional cultures (e.g. phage susceptibility, metabolic properties that are of biotechnological or industrial importance) would also be regarded as exceptionalness. Should this be the case, the authors must send a cover letter to the editor explaining why this new species deserves to be published in the journal, clarifying the uniqueness of the findings and the exceptionality of the new isolate. The manuscript should be submitted as a short communication with a maximum length of 2,000 words, including an abstract and references. Longer manuscripts with single strain descriptions may be allowed if they are accompanied by experimental information that is not necessarily of taxonomic value, but that may complement the taxonomic study (e.g. resistance to heavy metals, unexpected metabolic or genetic properties). Manuscripts not fulfilling the length and the exceptionality requirements will be directly rejected.

    Organisation and style of taxonomic papers dealing with new classifications. In order to provide a succinct description of the new taxon, the manuscript should not be divided into sections in the body of the text (except for the Acknowledgements and References). The body of the text (compulsory for single strain species descriptions, and voluntary for other taxonomic papers) should be a continuous article that starts from the introduction to the subject, followed by clarification of the methods and the results obtained, and finishing with the protologue that should include the correct etymology and the strain deposition numbers from two international collections.

    Use of word processing software

    It is important that the file be saved in the native format of the word processor used. The text should be in single-column format. Keep the layout of the text as simple as possible. Most formatting codes will be removed and replaced on processing the article. In particular, do not use the word processor's options to justify text or to hyphenate words. However, do use bold face, italics, subscripts, superscripts etc. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. If no grid is used, use tabs, not spaces, to align columns. The electronic text should be prepared in a way very similar to that of conventional manuscripts (see also the Guide to Publishing with Elsevier: http://www.elsevier.com/guidepublication). Note that source files of figures, tables and text graphics will be required whether or not you embed your figures in the text. See also the section on Electronic artwork.
    To avoid unnecessary errors you are strongly advised to use the 'spell-check' and 'grammar-check' functions of your word processor.

    Article structure

    Subdivision - unnumbered sections
    Divide your article into clearly defined sections. Each subsection is given a brief heading. Each heading should appear on its own separate line. Subsections should be used as much as possible when cross-referencing text: refer to the subsection by heading as opposed to simply 'the text'.

    Introduction
    State the objectives of the work and provide an adequate background, avoiding a detailed literature survey or a summary of the results.

    Material and methods
    Provide sufficient detail to allow the work to be reproduced. Methods already published should be indicated by a reference: only relevant modifications should be described.

    Results
    Results should be clear and concise.

    Discussion
    This should explore the significance of the results of the work, not repeat them. A combined Results and Discussion section is often appropriate. Avoid extensive citations and discussion of published literature.

    Conclusions
    The main conclusions of the study may be presented in a short Conclusions section, which may stand alone or form a subsection of a Discussion or Results and Discussion section.

    Appendices
    If there is more than one appendix, they should be identified as A, B, etc. Formulae and equations in appendices should be given separate numbering: Eq. (A.1), Eq. (A.2), etc.; in a subsequent appendix, Eq. (B.1) and so on. Similarly for tables and figures: Table A.1; Fig. A.1, etc.

    Essential title page information

    Title. Concise and informative. Titles are often used in information-retrieval systems. Avoid abbreviations and formulae where possible.
    Author names and affiliations. Where the family name may be ambiguous (e.g., a double name), please indicate this clearly. Present the authors' affiliation addresses (where the actual work was done) below the names. Indicate all affiliations with a lower-case superscript letter immediately after the author's name and in front of the appropriate address. Provide the full postal address of each affiliation, including the country name and, if available, the e-mail address of each author.
    Corresponding author. Clearly indicate who will handle correspondence at all stages of refereeing and publication, also post-publication. Ensure that phone numbers (with country and area code) are provided in addition to the e-mail address and the complete postal address. Contact details must be kept up to date by the corresponding author.
    Present/permanent address. If an author has moved since the work described in the article was done, or was visiting at the time, a 'Present address' (or 'Permanent address') may be indicated as a footnote to that author's name. The address at which the author actually did the work must be retained as the main, affiliation address. Superscript Arabic numerals are used for such footnotes.

    Abstract

    A concise and factual abstract is required. The abstract should state briefly the purpose of the research, the principal results and major conclusions. An abstract is often presented separately from the article, so it must be able to stand alone. For this reason, References should be avoided, but if essential, then cite the author(s) and year(s). Also, non-standard or uncommon abbreviations should be avoided, but if essential they must be defined at their first mention in the abstract itself.

    Graphical abstract

    Although a graphical abstract is optional, its use is encouraged as it draws more attention to the online article. The graphical abstract should summarize the contents of the article in a concise, pictorial form designed to capture the attention of a wide readership. Graphical abstracts should be submitted as a separate file in the online submission system. Image size: Please provide an image with a minimum of 531 × 1328 pixels (h × w) or proportionally more. The image should be readable at a size of 5 × 13 cm using a regular screen resolution of 96 dpi. Preferred file types: TIFF, EPS, PDF or MS Office files. See http://www.elsevier.com/graphicalabstracts for examples.
    Authors can make use of Elsevier's Illustration and Enhancement service to ensure the best presentation of their images and in accordance with all technical requirements: Illustration Service.

    Highlights

    Highlights are a short collection of bullet points that convey the core findings of the article. Highlights are optional and should be submitted in a separate file in the online submission system. Please use 'Highlights' in the file name and include 3 to 5 bullet points (maximum 85 characters, including spaces, per bullet point). See http://www.elsevier.com/highlights for examples.

    Keywords

    Immediately after the abstract, provide a maximum of 6 keywords, using American spelling and avoiding general and plural terms and multiple concepts (avoid, for example, 'and', 'of'). Be sparing with abbreviations: only abbreviations firmly established in the field may be eligible. These keywords will be used for indexing purposes.

    Chemical compounds

    You can enrich your article by providing a list of chemical compounds studied in the article. The list of compounds will be used to extract relevant information from the NCBI PubChem Compound database and display it next to the online version of the article on ScienceDirect. You can include up to 10 names of chemical compounds in the article. For each compound, please provide the PubChem CID of the most relevant record as in the following example: Glutamic acid (PubChem CID:611). The PubChem CIDs can be found via http://www.ncbi.nlm.nih.gov/pccompound. Please position the list of compounds immediately below the 'Keywords' section. It is strongly recommended to follow the exact text formatting as in the example below:
    Chemical compounds studied in this article
    Ethylene glycol (PubChem CID: 174); Plitidepsin (PubChem CID: 44152164); Benzalkonium chloride (PubChem CID: 15865)
    More information is available at: http://www.elsevier.com/PubChem.

    Abbreviations

    Define abbreviations that are not standard in this field in a footnote to be placed on the first page of the article. Such abbreviations that are unavoidable in the abstract must be defined at their first mention there, as well as in the footnote. Ensure consistency of abbreviations throughout the article.

    Acknowledgements

    Collate acknowledgements in a separate section at the end of the article before the references and do not, therefore, include them on the title page, as a footnote to the title or otherwise. List here those individuals who provided help during the research (e.g., providing language help, writing assistance or proof reading the article, etc.).

    Units

    Follow internationally accepted rules and conventions: use the international system of units (SI). If other units are mentioned, please give their equivalent in SI.

    Nucleotide sequence data

    New nucleotide data must be submitted and deposited in the DDBJ/EMBL/GenBank databases and an accession number obtained at the time of submission. Submission to any one of the three collaborating databanks is sufficient to ensure data entry in all. The accession number should be included in the manuscript e.g. as a footnote on the title page. For the evaluation of manuscripts containing gene sequences, sequence data have to be made available to the referees.

    Math formulae

    Present simple formulae in the line of normal text where possible and use the solidus (/) instead of a horizontal line for small fractional terms, e.g., X/Y. In principle, variables are to be presented in italics. Powers of e are often more conveniently denoted by exp. Number consecutively any equations that have to be displayed separately from the text (if referred to explicitly in the text).

    Footnotes

    Footnotes should be used sparingly. Number them consecutively throughout the article, using superscript Arabic numbers. Many wordprocessors build footnotes into the text, and this feature may be used. Should this not be the case, indicate the position of footnotes in the text and present the footnotes themselves separately at the end of the article. Do not include footnotes in the Reference list.
    Table footnotes
    Indicate each footnote in a table with a superscript lowercase letter.

    Artwork

    Electronic artwork
    General points
    • Make sure you use uniform lettering and sizing of your original artwork.
    • Embed the used fonts if the application provides that option.
    • Aim to use the following fonts in your illustrations: Arial, Courier, Times New Roman, Symbol, or use fonts that look similar.
    • Number the illustrations according to their sequence in the text.
    • Use a logical naming convention for your artwork files.
    • Provide captions to illustrations separately.
    • Size the illustrations close to the desired dimensions of the printed version.
    • Submit each illustration as a separate file.
    A detailed guide on electronic artwork is available on our website:
    http://www.elsevier.com/artworkinstructions
    You are urged to visit this site; some excerpts from the detailed information are given here.
    Formats
    If your electronic artwork is created in a Microsoft Office application (Word, PowerPoint, Excel) then please supply 'as is' in the native document format.
    Regardless of the application used other than Microsoft Office, when your electronic artwork is finalized, please 'Save as' or convert the images to one of the following formats (note the resolution requirements for line drawings, halftones, and line/halftone combinations given below):
    EPS (or PDF): Vector drawings, embed all used fonts.
    TIFF (or JPEG): Color or grayscale photographs (halftones), keep to a minimum of 300 dpi.
    TIFF (or JPEG): Bitmapped (pure black & white pixels) line drawings, keep to a minimum of 1000 dpi.
    TIFF (or JPEG): Combinations bitmapped line/half-tone (color or grayscale), keep to a minimum of 500 dpi.
    Please do not:
    • Supply files that are optimized for screen use (e.g., GIF, BMP, PICT, WPG); these typically have a low number of pixels and limited set of colors;
    • Supply files that are too low in resolution;
    • Submit graphics that are disproportionately large for the content.

    Color artwork
    Please make sure that artwork files are in an acceptable format (TIFF (or JPEG), EPS (or PDF), or MS Office files) and with the correct resolution. If, together with your accepted article, you submit usable color figures then Elsevier will ensure, at no additional charge, that these figures will appear in color on the Web (e.g., ScienceDirect and other sites) regardless of whether or not these illustrations are reproduced in color in the printed version. For color reproduction in print, you will receive information regarding the costs from Elsevier after receipt of your accepted article. Please indicate your preference for color: in print or on the Web only. For further information on the preparation of electronic artwork, please see http://www.elsevier.com/artworkinstructions.
    Please note: Because of technical complications that can arise by converting color figures to 'gray scale' (for the printed version should you not opt for color in print) please submit in addition usable black and white versions of all the color illustrations.

    Illustration services

    Elsevier's WebShop (http://webshop.elsevier.com/illustrationservices) offers Illustration Services to authors preparing to submit a manuscript but concerned about the quality of the images accompanying their article. Elsevier's expert illustrators can produce scientific, technical and medical-style images, as well as a full range of charts, tables and graphs. Image 'polishing' is also available, where our illustrators take your image(s) and improve them to a professional standard. Please visit the website to find out more.

    Figure captions
    Ensure that each illustration has a caption. Supply captions separately, not attached to the figure. A caption should comprise a brief title (not on the figure itself) and a description of the illustration. Keep text in the illustrations themselves to a minimum but explain all symbols and abbreviations used.

    Tables

    Number tables consecutively in accordance with their appearance in the text. Place footnotes to tables below the table body and indicate them with superscript lowercase letters. Avoid vertical rules. Be sparing in the use of tables and ensure that the data presented in tables do not duplicate results described elsewhere in the article.

    References

    Citation in text
    Please ensure that every reference cited in the text is also present in the reference list (and vice versa). Any references cited in the abstract must be given in full. Unpublished results and personal communications are not recommended in the reference list, but may be mentioned in the text. If these references are included in the reference list they should follow the standard reference style of the journal and should include a substitution of the publication date with either 'Unpublished results' or 'Personal communication'. Citation of a reference as 'in press' implies that the item has been accepted for publication.

    Reference links
    Increased discoverability of research and high quality peer review are ensured by online links to the sources cited. In order to allow us to create links to abstracting and indexing services, such as Scopus, CrossRef and PubMed, please ensure that data provided in the references are correct. Please note that incorrect surnames, journal/book titles, publication year and pagination may prevent link creation. When copying references, please be careful as they may already contain errors. Use of the DOI is encouraged.

    Web references
    As a minimum, the full URL should be given and the date when the reference was last accessed. Any further information, if known (DOI, author names, dates, reference to a source publication, etc.), should also be given. Web references can be listed separately (e.g., after the reference list) under a different heading if desired, or can be included in the reference list.

    References in a special issue
    Please ensure that the words 'this issue' are added to any references in the list (and any citations in the text) to other articles in the same Special Issue.

    Reference style
    References in the text should be:
    _ Mitchell and Catalan [6] and Baird-Parker [2]
    _...was discussed [6, 2]
    _Ventosa et aI. [10] described
    Reference to literature: The List of References should appear in alphabetical order, numbered consecutively with Arabic numbers.
    Formats for citations:
    [1] Fell, J.W., Boekhout, T., Fonseca, A., Scorzetti, G., Statzell-Tallman, A. (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evolution. Microbiol. 50, 1351–1371.
    [2] Fell, J.W., Statzell-Tallman, A. (1998) Cryptococcus Vuillemin. In: Kurtzman, C.P., Fell J.W. (Eds.), The Yeasts, A taxonomic study. Fourth Revised and Enlarged Edition, Elsevier, Amsterdam, pp. 742–767.
    [3] Fry, M. (1987) A detailed characterisation of soils under different fynbos-climate-geology combinations in the southwesern Cape. M.Sc. Thesis, Department of Soil Science, University of Stellenbosch, Stellenbosch, South Africa.

    Journal abbreviations source
    Journal names should be abbreviated according to the List of Title Word Abbreviations: http://www.issn.org/services/online-services/access-to-the-ltwa/.

    Video data

    Elsevier accepts video material and animation sequences to support and enhance your scientific research. Authors who have video or animation files that they wish to submit with their article are strongly encouraged to include links to these within the body of the article. This can be done in the same way as a figure or table by referring to the video or animation content and noting in the body text where it should be placed. All submitted files should be properly labeled so that they directly relate to the video file's content. In order to ensure that your video or animation material is directly usable, please provide the files in one of our recommended file formats with a preferred maximum size of 50 MB. Video and animation files supplied will be published online in the electronic version of your article in Elsevier Web products, including ScienceDirect: http://www.sciencedirect.com. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. These will be used instead of standard icons and will personalize the link to your video data. For more detailed instructions please visit our video instruction pages at http://www.elsevier.com/artworkinstructions. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content.

    Supplementary data

    Elsevier accepts electronic supplementary material to support and enhance your scientific research. Supplementary files offer the author additional possibilities to publish supporting applications, high-resolution images, background datasets, sound clips and more. Supplementary files supplied will be published online alongside the electronic version of your article in Elsevier Web products, including ScienceDirect: http://www.sciencedirect.com. In order to ensure that your submitted material is directly usable, please provide the data in one of our recommended file formats. Authors should submit the material in electronic format together with the article and supply a concise and descriptive caption for each file. For more detailed instructions please visit our artwork instruction pages at http://www.elsevier.com/artworkinstructions.

    Data at PANGAEA

    Electronic archiving of supplementary data enables readers to replicate, verify and build upon the conclusions published in your paper. We recommend that data should be deposited in the data library PANGAEA (http://www.pangaea.de). Data are quality controlled and archived by an editor in standard machine-readable formats and are available via Open Access. After processing, the author receives an identifier (DOI) linking to the supplements for checking. As your data sets will be citable you might want to refer to them in your article. In any case, data supplements and the article will be automatically linked as in the following example: doi:10.1016/0016-7037(95)00105-9. Please use PANGAEA's web interface to submit your data (http://www.pangaea.de/submit/).

    Data deposit and linking

    Elsevier encourages authors to deposit raw data sets underpinning their research publication in data repositories, and to enable interlinking of articles and data. Please visit http://www.elsevier.com/databaselinking for more information on depositing and linking your data with a supported data repository.

    Google Maps and KML files

    KML (Keyhole Markup Language) files (optional): You can enrich your online articles by providing KML or KMZ files which will be visualized using Google maps. The KML or KMZ files can be uploaded in our online submission system. KML is an XML schema for expressing geographic annotation and visualization within Internet-based Earth browsers. Elsevier will generate Google Maps from the submitted KML files and include these in the article when published online. Submitted KML files will also be available for downloading from your online article on ScienceDirect. For more information see http://www.elsevier.com/googlemaps.

    Mol files

    Mol files (optional): Elsevier would like to enrich your online article by visualizing and providing details of chemical structures you define as the main chemical compounds described. For this purpose, corresponding mol files can be uploaded via the online submission system. Each compound needs to be submitted as a separate mol file. Please use your preferred drawing tool to export chemical structures as mol files and ensure that they are unique, complete and do not contain any R-groups or other variables so that a correct InChI key can be generated. For more information see http://www.elsevier.com/mol.

    Interactive Phylogenetic Trees

    You can enrich your online articles by providing phylogenetic tree data files (optional) in Newick or NeXML format, which will be visualized using the interactive tree viewer embedded within the online article. Using the viewer it will be possible to zoom into certain tree areas, change the tree layout, search within the tree, and collapse/expand tree nodes and branches. Submitted tree files will also be available for downloading from your online article on ScienceDirect. Each tree must be contained in an individual data file before being uploaded separately to the online submission system, via the 'phylogenetic tree data' submission category. Newick files must have the extension .new or .nwk (note that a semicolon is needed to end the tree). Please do not enclose comments in Newick files and also delete any artificial line breaks within the tree data because these will stop the tree from showing. For NeXML, the file extension should be .xml. Please do not enclose comments in the file. Tree data submitted with other file extensions will not be processed. Please make sure that you validate your Newick/NeXML files prior to submission. For more information please see http://www.elsevier.com/phylogenetictrees.

    Submission checklist

    The following list will be useful during the final checking of an article prior to sending it to the journal for review. Please consult this Guide for Authors for further details of any item.
    Ensure that the following items are present:
    One author has been designated as the corresponding author with contact details:
    • E-mail address
    • Full postal address
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    All necessary files have been uploaded, and contain:
    • Keywords
    • All figure captions
    • All tables (including title, description, footnotes)
    Further considerations
    • Manuscript has been 'spell-checked' and 'grammar-checked'
    • References are in the correct format for this journal
    • All references mentioned in the Reference list are cited in the text, and vice versa
    • Permission has been obtained for use of copyrighted material from other sources (including the Web)
    • Color figures are clearly marked as being intended for color reproduction on the Web (free of charge) and in print, or to be reproduced in color on the Web (free of charge) and in black-and-white in print
    • If only color on the Web is required, black-and-white versions of the figures are also supplied for printing purposes
    For any further information please visit our customer support site at http://support.elsevier.com.

    Use of the Digital Object Identifier

    The Digital Object Identifier (DOI) may be used to cite and link to electronic documents. The DOI consists of a unique alpha-numeric character string which is assigned to a document by the publisher upon the initial electronic publication. The assigned DOI never changes. Therefore, it is an ideal medium for citing a document, particularly 'Articles in press' because they have not yet received their full bibliographic information. Example of a correctly given DOI (in URL format; here an article in the journal Physics Letters B):
    http://dx.doi.org/10.1016/j.physletb.2010.09.059
    When you use a DOI to create links to documents on the web, the DOIs are guaranteed never to change.

    Proofs

    One set of page proofs (as PDF files) will be sent by e-mail to the corresponding author (if we do not have an e-mail address then paper proofs will be sent by post) or, a link will be provided in the e-mail so that authors can download the files themselves. Elsevier now provides authors with PDF proofs which can be annotated; for this you will need to download Adobe Reader version 9 (or higher) available free from http://get.adobe.com/reader. Instructions on how to annotate PDF files will accompany the proofs (also given online). The exact system requirements are given at the Adobe site: http://www.adobe.com/products/reader/tech-specs.html.
    If you do not wish to use the PDF annotations function, you may list the corrections (including replies to the Query Form) and return them to Elsevier in an e-mail. Please list your corrections quoting line number. If, for any reason, this is not possible, then mark the corrections and any other comments (including replies to the Query Form) on a printout of your proof and return by fax, or scan the pages and e-mail, or by post. Please use this proof only for checking the typesetting, editing, completeness and correctness of the text, tables and figures. Significant changes to the article as accepted for publication will only be considered at this stage with permission from the Editor. We will do everything possible to get your article published quickly and accurately. It is important to ensure that all corrections are sent back to us in one communication: please check carefully before replying, as inclusion of any subsequent corrections cannot be guaranteed. Proofreading is solely your responsibility.

    Offprints

    The corresponding author, at no cost, will be provided with a personalized link providing 50 days free access to the final published version of the article on ScienceDirect. This link can also be used for sharing via email and social networks. For an extra charge, paper offprints can be ordered via the offprint order form which is sent once the article is accepted for publication. Both corresponding and co-authors may order offprints at any time via Elsevier's WebShop (http://webshop.elsevier.com/myarticleservices/offprints). Authors requiring printed copies of multiple articles may use Elsevier WebShop's 'Create Your Own Book' service to collate multiple articles within a single cover (http://webshop.elsevier.com/myarticleservices/booklets).



    You can track your submitted article at http://help.elsevier.com/app/answers/detail/a_id/89/p/8045/. You can track your accepted article at http://www.elsevier.com/trackarticle. You are also welcome to contact Customer Support via http://support.elsevier.com.

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