Guide for Authors

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    INTRODUCTION
    • Types of paper
    • Page charges
    BEFORE YOU BEGIN
    • Ethics in publishing
    • Human and animal rights
    • Policy and ethics
    • Conflict of interest
    • Submission declaration and verification
    • Changes to authorship
    • Copyright
    • Retained author rights
    • Role of the funding source
    • Funding body agreements and policies
    • Open access
    • Language (usage and editing services)
    • Submission
    • Submit your article
    • Referees
    PREPARATION
    • NEW SUBMISSIONS
    • References
    • Formatting requirements
    • REVISED SUBMISSIONS
    • Article structure
    • Essential title page information
    • Abstract
    • Graphical abstract
    • Highlights
    • Database linking
    • Footnotes
    • Artwork
    • Tables
    • References
    • Video data
    • AudioSlides
    • Supplementary data
    • Submission checklist
    AFTER ACCEPTANCE
    • Use of the Digital Object Identifier
    • Online proof correction
    • Offprints
    • Offprints
    • Additional information
    AUTHOR INQUIRIES

    Your Paper Your Way
    your paper your way



    EuPA Open Proteomics is published by Elsevier on behalf of the European Proteomics Association (EuPA) and is aimed at both European and international protein scientists.

    Expanding and complementing the society affiliate Journal of Proteomics, EuPA Open Proteomics will publish on all aspects of proteomics and cover the complete spectrum from basic to translational proteomics. Particularly welcome are reports on novel methodological and technical developments, including bioinformatics and data processing, mass spectrometry imaging, pathway analysis, peptidomics, lipidomics and glycomics. It will also accept direct submissions from authors wishing to report on large data sets (submitted to raw data repositories) and descriptive studies. Manuscripts are welcome from plant, animal, microbial and human studies. All manuscripts are strictly peer reviewed and conform the highest ethical standards. EuPA Open Proteomics also publishes official EuPA reports and notes.

    Types of paper

    The following types of paper are published:

    Original Articles: Original articles are the normal medium of publication. Although there is no fixed length, articles should be as concise as possible, while providing sufficient information for the work to be repeated and for the claims of the authors to be judged by the readers.

    Mandatory requirements for reporting of clinical biomarker studies:

    1) A clinical biomarker is only relevant in specific contexts of use per disease, it must have a potential to improve the current state of the art (either being of added value, or based on its sole performance), and its application must be linked to a clear change in patient management. As such, the specific proposed context of use of the presented biomarker must be clearly provided and the expected practical consequence of the biomarker application should be discussed.
    2) A biomarker can only be assessed in an independent (ideally blinded) test set, containing sufficient samples to demonstrate significant value and justify relevant claims regarding biomarker use. Assessment of performance in a discovery set is inappropriate.
    3) This initial independent validation and performance assessment has to be performed in samples that reflect the typical clinical situation depending on the targeted context of use.
    4) Authors submitting clinical biomarker studies should address the above points in the cover letter, so that the Editor can assess and evaluate if the submitted manuscript fulfils the requirements for publication in EuPA Open Proteomics.

    Mini-reviews: These are short reviews contributed by scientists who are leading specialists in their field of expertise. Authors wishing to contribute a review paper are advised first to contact the Editor in Chief.

    Short Communications:

    Technical reports: Technical reports should present brief descriptions of novel apparatus, a new experimental or computational method, test or procedure, or an improvement or noteworthy modification of an already existing technique or platform used in the proteomic workflow. Technical reports should show a realistic application of the methodology described. Theoretical papers dealing with mechanistic aspects of proteomic techniques will also be considered. A technical report should be a short (no more than two pages when published) description written in a continuous style with no more than two figures and one table.

    Data reports:

    News & Views: News & Views point out the author(s) vision of the character and importance of a new direction in proteome research. They are not intended to be accounts or analyses of an individual's personal research. Although News & Views will usually be invited, they can be submitted without invitation. Author(s) are encouraged to suggest experts in the field who can act as reviewers.

    Letters to the Editor: Letters to the Editor are intended to stimulate discussion and debate in areas of general concern and controversy in proteomics, and generally reflect the personal opinions of the author(s). They should be written in a continuous style and should normally not exceed two printed pages and contain no more than one figure or table.

    Page charges

    This title is fully open access and therefore funded not through library subscription payments but through author fees. If you would like your article to be published open access, but you genuinely cannot afford these fees, then individual waiver requests are considered on a case-by-case basis and may be granted in cases of genuine need. Priority for this waiver programme will be given to applications by authors from countries eligible for the Research4Life programme. See http://www.research4life.org/institutions.html.

    Ethics in publishing

    For information on Ethics in publishing and Ethical guidelines for journal publication see http://www.elsevier.com/publishingethics and http://www.elsevier.com/journal-authors/ethics.

    Human and animal rights

    If the work involves the use of animal or human subjects, the author should ensure that the work described has been carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) for experiments involving humans http://www.wma.net/en/30publications/10policies/b3/index.html; EU Directive 2010/63/EU for animal experiments http://ec.europa.eu/environment/chemicals/lab_animals/legislation_en.htm; Uniform Requirements for manuscripts submitted to Biomedical journals http://www.icmje.org. Authors should include a statement in the manuscript that informed consent was obtained for experimentation with human subjects. The privacy rights of human subjects must always be observed.

    Policy and ethics

    The work described in your article must have been carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) for experiments involving humans http://www.wma.net/en/30publications/10policies/b3/index.html; EU Directive 2010/63/EU for animal experiments http://ec.europa.eu/environment/chemicals/lab_animals/legislation_en.htm; Uniform Requirements for manuscripts submitted to Biomedical journals http://www.icmje.org. This must be stated at an appropriate point in the article.

    EuPA Open Proteomics will reject any paper where there is reason to believe that animals have been subjected to unnecessary or avoidable pain or distress.

    Conflict of interest

    EuPA Open Proteomics follows the ICMJE recommendations regarding conflict of interest disclosures. All authors are required to report the following information with each submission: (1) All third-party financial support for the work in the submitted manuscript. (2) All financial relationships with any entities that could be viewed as relevant to the general area of the submitted manuscript. (3) All sources of revenue with relevance to the submitted work who made payments to you, or to your institution on your behalf, in the 36 months prior to submission. (4) Any other interactions with the sponsor of outside of the submitted work should also be reported. (5) Any relevant patents or copyrights (planned, pending, or issued). (6) Any other relationships or affiliations that may be perceived by readers to have influenced, or give the appearance of potentially influencing, what you wrote in the submitted work. As a general guideline, it is usually better to disclose a relationship than not. This information will be acknowledged at publication in a Transparency Document link directly in the article. Additional information on the ICMJE recommendations can be found at: http://www.icmje.org/. The form for conflict of interest disclosure can be downloaded here: http://www.icmje.org/coi_disclosure.pdf (if this link does not display properly in your browser, please right-click the link and select "Save Target As..." or "Save Link as..." from the pop-up menu).

    Authorship
    All authors should have made substantial contributions to all of the following: (1) the conception and design of the study, or acquisition of data, or analysis and interpretation of data, (2) drafting the article or revising it critically for important intellectual content, (3) final approval of the version to be submitted.

    Submission declaration and verification

    Submission of an article implies that the work described has not been published previously (except in the form of an abstract or as part of a published lecture or academic thesis or as an electronic preprint, see http://www.elsevier.com/postingpolicy), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder. To verify originality, your article may be checked by the originality detection service CrossCheck http://www.elsevier.com/editors/plagdetect.

    Changes to authorship

    This policy concerns the addition, deletion, or rearrangement of author names in the authorship of accepted manuscripts:
    Before the accepted manuscript is published in an online issue: Requests to add or remove an author, or to rearrange the author names, must be sent to the Journal Manager from the corresponding author of the accepted manuscript and must include: (a) the reason the name should be added or removed, or the author names rearranged and (b) written confirmation (e-mail, fax, letter) from all authors that they agree with the addition, removal or rearrangement. In the case of addition or removal of authors, this includes confirmation from the author being added or removed. Requests that are not sent by the corresponding author will be forwarded by the Journal Manager to the corresponding author, who must follow the procedure as described above. Note that: (1) Journal Managers will inform the Journal Editors of any such requests and (2) publication of the accepted manuscript in an online issue is suspended until authorship has been agreed.
    After the accepted manuscript is published in an online issue: Any requests to add, delete, or rearrange author names in an article published in an online issue will follow the same policies as noted above and result in a corrigendum.

    Copyright

    Authors will be asked to sign an exclusive publishing agreement that allows them to retain copyright of their work. Authors can choose to publish their work under one of two Creative Common licenses. The first option is the Creative Commons Attribution 3.0 Unported License (or CC BY), which allows users to alter and build upon the article and then distribute the resulting work, even commercially. As with all Creative Commons licenses the work must be attributed to the original author and publisher. This license encourages maximum use and redistribution. The full details of the license are available at http://creativecommons.org/licenses/by/3.0/legalcode. The second option is the Creative Commons Attribution-Noncommercial-ShareAlike Works 3.0 Unported License (or CC BY-NC-SA), which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. The full details of the license are available at http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. To provide open access, expenses are offset by a publication fee of 1,700 EUR that will allow EuPA Open Proteomics to support itself in a fully sustainable way. Students from affiliated societies receive a discount.

    Retained author rights

    Authors retain the copyright of their work.

    Role of the funding source

    You are requested to identify who provided financial support for the conduct of the research and/or preparation of the article and to briefly describe the role of the sponsor(s), if any, in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. If the funding source(s) had no such involvement then this should be stated.

    Funding body agreements and policies

    Elsevier has established agreements and developed policies to allow authors whose articles appear in journals published by Elsevier, to comply with potential manuscript archiving requirements as specified as conditions of their grant awards. To learn more about existing agreements and policies please visit http://www.elsevier.com/fundingbodies.

    Open access

    EuPA Open Proteomics follows an author-pays open access model. The open access publication fee for this journal is $1,200 for EuPA affiliated students and $1,700 for all others.

    Language (usage and editing services)

    Please write your text in good English (American or British usage is accepted, but not a mixture of these). Authors who feel their English language manuscript may require editing to eliminate possible grammatical or spelling errors and to conform to correct scientific English may wish to use the English Language Editing service available from Elsevier's WebShop (http://webshop.elsevier.com/languageediting/) or visit our customer support site (http://support.elsevier.com) for more information.

    Submission

    Submission to this journal proceeds totally online and you will be guided stepwise through the creation and uploading of your files. The system automatically converts source files to a single PDF file of the article, which is used in the peer-review process. Please note that even though manuscript source files are converted to PDF files at submission for the review process, these source files are needed for further processing after acceptance. All correspondence, including notification of the Editor's decision and requests for revision, takes place by e-mail removing the need for a paper trail.

    Submit your article

    Please submit your article via http://ees.elsevier.com/euprot.

    Referees

    Please provide the names and addresses of 4 - 5 suitable potential reviewers. If there are compelling reasons for excluding some individuals as potential reviewers, these can be mentioned. However, choice of reviewers is at the Editors' discretion.

    NEW SUBMISSIONS

    Submission to this journal proceeds totally online and you will be guided stepwise through the creation and uploading of your files. The system automatically converts your files to a single PDF file, which is used in the peer-review process.
    As part of the Your Paper Your Way service, you may choose to submit your manuscript as a single file to be used in the refereeing process. This can be a PDF file or a Word document, in any format or lay-out that can be used by referees to evaluate your manuscript. It should contain high enough quality figures for refereeing. If you prefer to do so, you may still provide all or some of the source files at the initial submission. Please note that individual figure files larger than 10 MB must be uploaded separately.

    References

    There are no strict requirements on reference formatting at submission. References can be in any style or format as long as the style is consistent. Where applicable, author(s) name(s), journal title/book title, chapter title/article title, year of publication, volume number/book chapter and the pagination must be present. Use of DOI is highly encouraged. The reference style used by the journal will be applied to the accepted article by Elsevier at the proof stage. Note that missing data will be highlighted at proof stage for the author to correct.

    Formatting requirements

    There are no strict formatting requirements but all manuscripts must contain the essential elements needed to convey your manuscript, for example Abstract, Keywords, Introduction, Materials and Methods, Results, Conclusions, Artwork and Tables with Captions.
    If your article includes any Videos and/or other Supplementary material, this should be included in your initial submission for peer review purposes.
    Divide the article into clearly defined sections.

    Figures and tables embedded in text
    Please ensure the figures and the tables included in the single file are placed next to the relevant text in the manuscript, rather than at the bottom or the top of the file.

    REVISED SUBMISSIONS

    To assist in the reviewing of your paper, please add line numbering to your manuscript file.

    Use of word processing software
    Regardless of the file format of the original submission, at revision you must provide us with an editable file of the entire article. Keep the layout of the text as simple as possible. Most formatting codes will be removed and replaced on processing the article. The electronic text should be prepared in a way very similar to that of conventional manuscripts (see also the Guide to Publishing with Elsevier: http://www.elsevier.com/guidepublication). See also the section on Electronic artwork.
    To avoid unnecessary errors you are strongly advised to use the 'spell-check' and 'grammar-check' functions of your word processor.

    Article structure

    Original articles are usually divided into the sections Introduction, Materials and methods, Results, Discussion and Conclusions:

    Introduction
    This is a short section in which the authors should state the reasons for performing the work, with brief reference to relevant previous work.

    Material and methods
    Provide sufficient detail to allow the work to be reproduced. Methods already published should be indicated by a reference: only relevant modifications should be described.

    Results
    Results should be clear and concise.

    Discussion
    This should explore the significance of the results of the work, not repeat them. A combined Results and Discussion section is often appropriate. Avoid extensive citations and discussion of published literature.

    Conclusions
    The main conclusions of the study may be presented in a short Conclusions section, which may stand alone or form a subsection of a Discussion or Results and Discussion section.

    Technical reports, News & Views, Letters to the Editor
    These types of papers are not divided into sections after the summary, except for the reference list. The first paragraph serves as an introduction; acknowledgments are added as a final paragraph before the reference list.

    Essential title page information

    Title. Concise and informative. Titles are often used in information-retrieval systems. Avoid abbreviations and formulae where possible.
    Author names and affiliations. Where the family name may be ambiguous (e.g., a double name), please indicate this clearly. Present the authors' affiliation addresses (where the actual work was done) below the names. Indicate all affiliations with a lower-case superscript letter immediately after the author's name and in front of the appropriate address. Provide the full postal address of each affiliation, including the country name and, if available, the e-mail address of each author.
    Corresponding author. Clearly indicate who will handle correspondence at all stages of refereeing and publication, also post-publication. Ensure that phone numbers (with country and area code) are provided in addition to the e-mail address and the complete postal address. Contact details must be kept up to date by the corresponding author.
    Present/permanent address. If an author has moved since the work described in the article was done, or was visiting at the time, a 'Present address' (or 'Permanent address') may be indicated as a footnote to that author's name. The address at which the author actually did the work must be retained as the main, affiliation address. Superscript Arabic numerals are used for such footnotes.

    Abstract

    A concise and factual abstract is required. The abstract should state briefly the purpose of the research, the principal results and major conclusions. An abstract is often presented separately from the article, so it must be able to stand alone. For this reason, References should be avoided, but if essential, then cite the author(s) and year(s). Also, non-standard or uncommon abbreviations should be avoided, but if essential they must be defined at their first mention in the abstract itself.

    Graphical abstract

    A Graphical abstract is mandatory for this journal. It should summarize the contents of the article in a concise, pictorial form designed to capture the attention of a wide readership online. Authors must provide images that clearly represent the work described in the article. Graphical abstracts should be submitted as a separate file in the online submission system. Image size: please provide an image with a minimum of 531 × 1328 pixels (h × w) or proportionally more. The image should be readable at a size of 5 × 13 cm using a regular screen resolution of 96 dpi. Preferred file types: TIFF, EPS, PDF or MS Office files. See http://www.elsevier.com/graphicalabstracts for examples.
    Authors can make use of Elsevier's Illustration and Enhancement service to ensure the best presentation of their images also in accordance with all technical requirements: Illustration Service.

    Graphical Abstract should allow readers to quickly gain an understanding of the main take-home message of the paper and is intended to encourage browsing, promote interdisciplinary scholarship, and help readers identify more quickly which papers are most relevant to their research interests. The Graphical Abstract should summarize the contents of the paper in a concise, pictorial form designed to capture the attention of a wide readership. Authors must provide images that clearly represent the work described in the paper. A key, summarising figure taken from the original paper can also be submitted as a graphical abstract.

    Graphical Abstracts should be submitted as a separate file in EES by selecting "Graphical Abstract" from the drop-down box when uploading files.

    Highlights

    Highlights are mandatory for this journal. They consist of a short collection of bullet points that convey the core findings of the article and should be submitted in a separate file in the online submission system. Please use 'Highlights' in the file name and include 3 to 5 bullet points (maximum 85 characters, including spaces, per bullet point). See http://www.elsevier.com/highlights for examples.

    Database linking

    Elsevier encourages authors to connect articles with external databases, giving their readers one-click access to relevant databases that help to build a better understanding of the described research. Please refer to relevant database identifiers using the following format in your article: Database: xxxx (e.g., TAIR: AT1G01020; CCDC: 734053; PDB: 1XFN). See http://www.elsevier.com/databaselinking for more information and a full list of supported databases.

    Footnotes

    Footnotes should be used sparingly. Number them consecutively throughout the article. Many wordprocessors build footnotes into the text, and this feature may be used. Should this not be the case, indicate the position of footnotes in the text and present the footnotes themselves separately at the end of the article. Do not include footnotes in the Reference list.
    Table footnotes
    Indicate each footnote in a table with a superscript lowercase letter.

    Artwork

    Electronic artwork
    General points
    • Make sure you use uniform lettering and sizing of your original artwork.
    • Preferred fonts: Arial (or Helvetica), Times New Roman (or Times), Symbol, Courier.
    • Number the illustrations according to their sequence in the text.
    • Use a logical naming convention for your artwork files.
    • Indicate per figure if it is a single, 1.5 or 2-column fitting image.
    • For Word submissions only, you may still provide figures and their captions, and tables within a single file at the revision stage.
    • Please note that individual figure files larger than 10 MB must be provided in separate source files.
    A detailed guide on electronic artwork is available on our website:
    http://www.elsevier.com/artworkinstructions.
    You are urged to visit this site; some excerpts from the detailed information are given here.
    Formats
    Regardless of the application used, when your electronic artwork is finalized, please 'save as' or convert the images to one of the following formats (note the resolution requirements for line drawings, halftones, and line/halftone combinations given below):
    EPS (or PDF): Vector drawings. Embed the font or save the text as 'graphics'.
    TIFF (or JPG): Color or grayscale photographs (halftones): always use a minimum of 300 dpi.
    TIFF (or JPG): Bitmapped line drawings: use a minimum of 1000 dpi.
    TIFF (or JPG): Combinations bitmapped line/half-tone (color or grayscale): a minimum of 500 dpi is required.
    Please do not:
    • Supply files that are optimized for screen use (e.g., GIF, BMP, PICT, WPG); the resolution is too low.
    • Supply files that are too low in resolution.
    • Submit graphics that are disproportionately large for the content.

    Figure captions
    Ensure that each illustration has a caption. A caption should comprise a brief title (not on the figure itself) and a description of the illustration. Keep text in the illustrations themselves to a minimum but explain all symbols and abbreviations used.

    Tables

    Number tables consecutively in accordance with their appearance in the text. Place footnotes to tables below the table body and indicate them with superscript lowercase letters. Avoid vertical rules. Be sparing in the use of tables and ensure that the data presented in tables do not duplicate results described elsewhere in the article.

    References

    Citation in text
    The numerical system of references should be used. References in the text should be cited by numbers in square brackets in the order of their citation.

    References are listed together in their order of appearance in a separate section at the end of the text under the heading References. All references should be numbered consecutively. References to journals should contain initials and names of all authors, article title, abbreviation of the name of the journal according to the List of Serial Title World Abbreviations (International Series Data System, 20, rue Bachaumont, 75002 Paris, France. ISBN 2-904938-02-8), year of publication, volume number, and page numbers. References to books should also include the title (of series and volumes), initials and names of the editor(s), the publisher and place of publication.

    Examples:

    Reference to a journal publication:

    [1] Resing KA, Ahn NG. Proteomics strategies for protein identification. FEBS Letters 2005;579:885-9.

    Reference to a book:

    [2] Rehm H. Protein Biochemistry and Proteomics. San Diego: Academic Press/Elsevier Inc; 2006.

    Reference to a chapter in an edited book or book series:

    [3] Morgan JW, Hettick JM, Russell DH. Peptide sequencing by MALDI 193-nm photodissociation TOF MS. In: Burlingame AL, editor. Methods in Enzymology, vol 402: Biological Mass Spectrometry. San Diego: Academic Press/Elsevier Inc; 2005, p.186-209.

    Reference to a paper as "in press" implies that it has been accepted for publication. Evidence (e.g., a photocopy of the note of acceptance from the journal concerned) should accompany the submitted typescript. Papers that are "in press" should be included as a number in the text. Other papers submitted before or simultaneously with the paper in question should be included as a number in the text and in the References section, stating the name of the journal. Copies of papers that are submitted elsewhere should be provided for inspection by the Editors. Omission of this information will delay publication and may lead to redating of a submitted manuscript. Papers presented at scientific meetings that are not available in published form should not be cited as references in the References section.

    Unpublished results should not be listed in the References section. In the text they are mentioned as follows: "(Tervoort MV and Glimcher J, unpublished data)". When unpublished results are cited, the data should be provided for the Editors' information when essential for proper evaluation, or if requested.

    A personal communication should be mentioned in the text as follows: "(Tervoort MV, personal communication)". Authors should not make unauthorized use of personal communications. Personal communications are not to be included in the Reference section.

    Reference management software
    This journal has standard templates available in key reference management packages EndNote (http://www.endnote.com/support/enstyles.asp) and Reference Manager (http://refman.com/support/rmstyles.asp). Using plug-ins to wordprocessing packages, authors only need to select the appropriate journal template when preparing their article and the list of references and citations to these will be formatted according to the journal style which is described below.

    Reference formatting
    There are no strict requirements on reference formatting at submission. References can be in any style or format as long as the style is consistent. Where applicable, author(s) name(s), journal title/book title, chapter title/article title, year of publication, volume number/book chapter and the pagination must be present. Use of DOI is highly encouraged. The reference style used by the journal will be applied to the accepted article by Elsevier at the proof stage. Note that missing data will be highlighted at proof stage for the author to correct. If you do wish to format the references yourself they should be arranged according to the following examples:

    Reference style
    Text: Indicate references by number(s) in square brackets in line with the text. The actual authors can be referred to, but the reference number(s) must always be given.
    List: Number the references (numbers in square brackets) in the list in the order in which they appear in the text.
    Examples:
    Reference to a journal publication:
    [1] Van der Geer J, Hanraads JAJ, Lupton RA. The art of writing a scientific article. J Sci Commun 2010;163:51–9.
    Reference to a book:
    [2] Strunk Jr W, White EB. The elements of style. 4th ed. New York: Longman; 2000.
    Reference to a chapter in an edited book:
    [3] Mettam GR, Adams LB. How to prepare an electronic version of your article. In: Jones BS, Smith RZ, editors. Introduction to the electronic age, New York: E-Publishing Inc; 2009, p. 281–304.
    Note shortened form for last page number. e.g., 51–9, and that for more than 6 authors the first 6 should be listed followed by 'et al.' For further details you are referred to 'Uniform Requirements for Manuscripts submitted to Biomedical Journals' (J Am Med Assoc 1997;277:927–34) (see also http://www.nlm.nih.gov/bsd/uniform_requirements.html).

    Video data

    Elsevier accepts video material and animation sequences to support and enhance your scientific research. Authors who have video or animation files that they wish to submit with their article are strongly encouraged to include links to these within the body of the article. This can be done in the same way as a figure or table by referring to the video or animation content and noting in the body text where it should be placed. All submitted files should be properly labeled so that they directly relate to the video file's content. In order to ensure that your video or animation material is directly usable, please provide the files in one of our recommended file formats with a preferred maximum size of 50 MB. Video and animation files supplied will be published online in the electronic version of your article in Elsevier Web products, including ScienceDirect: http://www.sciencedirect.com. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. These will be used instead of standard icons and will personalize the link to your video data. For more detailed instructions please visit our video instruction pages at http://www.elsevier.com/artworkinstructions. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content.

    AudioSlides

    The journal encourages authors to create an AudioSlides presentation with their published article. AudioSlides are brief, webinar-style presentations that are shown next to the online article on ScienceDirect. This gives authors the opportunity to summarize their research in their own words and to help readers understand what the paper is about. More information and examples are available at http://www.elsevier.com/audioslides. Authors of this journal will automatically receive an invitation e-mail to create an AudioSlides presentation after acceptance of their paper.

    Supplementary data

    Elsevier accepts electronic supplementary material to support and enhance your scientific research. Supplementary files offer the author additional possibilities to publish supporting applications, high-resolution images, background datasets, sound clips and more. Supplementary files supplied will be published online alongside the electronic version of your article in Elsevier Web products, including ScienceDirect: http://www.sciencedirect.com. In order to ensure that your submitted material is directly usable, please provide the data in one of our recommended file formats. Authors should submit the material in electronic format together with the article and supply a concise and descriptive caption for each file. For more detailed instructions please visit our artwork instruction pages at http://www.elsevier.com/artworkinstructions.

    Submission checklist

    The following list will be useful during the final checking of an article prior to sending it to the journal for review. Please consult this Guide for Authors for further details of any item.
    Ensure that the following items are present:
    One author has been designated as the corresponding author with contact details:
    • E-mail address
    • Full postal address
    • Telephone
    All necessary files have been uploaded, and contain:
    • Keywords
    • All figure captions
    • All tables (including title, description, footnotes)
    Further considerations
    • Manuscript has been 'spell-checked' and 'grammar-checked'
    • All references mentioned in the Reference list are cited in the text, and vice versa
    • Permission has been obtained for use of copyrighted material from other sources (including the Web)
    • Color figures are clearly marked as being intended for color reproduction on the Web (free of charge) and in print, or to be reproduced in color on the Web (free of charge) and in black-and-white in print
    • If only color on the Web is required, black-and-white versions of the figures are also supplied for printing purposes
    For any further information please visit our customer support site at http://support.elsevier.com.

    Use of the Digital Object Identifier

    The Digital Object Identifier (DOI) may be used to cite and link to electronic documents. The DOI consists of a unique alpha-numeric character string which is assigned to a document by the publisher upon the initial electronic publication. The assigned DOI never changes. Therefore, it is an ideal medium for citing a document, particularly 'Articles in press' because they have not yet received their full bibliographic information. Example of a correctly given DOI (in URL format; here an article in the journal Physics Letters B):
    http://dx.doi.org/10.1016/j.physletb.2010.09.059
    When you use a DOI to create links to documents on the web, the DOIs are guaranteed never to change.

    Online proof correction

    Corresponding authors will receive an e-mail with a link to our online proofing system, allowing annotation and correction of proofs online. The environment is similar to MS Word: in addition to editing text, you can also comment on figures/tables and answer questions from the Copy Editor. Web-based proofing provides a faster and less error-prone process by allowing you to directly type your corrections, eliminating the potential introduction of errors.
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    Additional information

    Appendix

    Standard abbreviations allowed to be used without explanation or definition in all articles published in EuPA Open Proteomics.

    A absorbance

    ACES 2-[(2-amino-2-oxoethyl)amino] ethanesulphonic acid

    CAN acetonitrile

    A/D analog to digital converter

    AEBSF 4-(2-aminoethyl)benzenesulphonyl fluoride

    amu atomic mass unit

    ANOVA analysis of variance

    API atmospheric pressure ionization

    AUC area under curve

    Bis N,N'-methylenebisacrylamide

    bp base pairs

    BSA bovine serum albumin

    %C cross-linking agent (g/100 mL)/%T

    CAPS 3-(cyclohexylamino)-1-propanesulphonic acid

    CBB Coomassie Brilliant Blue

    CCD charge-coupled device

    CD circular dicroism

    CE capillary electrophoresis

    CEC capillary electrochromatography

    CFE continuous flow electrophoresis

    CHAPS 3-[(3-cholamidopropyl)dimethylamonio]-1-propanesulphonate

    CHCA ?-cyano-4-hydroxycinnamic acid

    CHES 2-(N-cyclohexylamino)ethanesulphonic acid

    CID collision-induced dissociation

    CIEF capillary isoelectric focusing

    CMC critical micelle concentration

    Con A Concanavalin A

    CNS central nervous system

    cpm counts per minute

    CTAB etyltrimethylammonium bromide

    CV coefficient of variation

    CZE capillary zone electrophoresis

    1-D one-dimensional

    2-D two-dimensional

    Da dalton (molecular mass)

    2-DE two-dimensional electrophoresis

    DIGE fluorescence difference gel electrophoresis

    DGGE denaturing gradient gel electrophoresis

    DMEM Dulbecco's modified Eagle medium

    DMF N,N-dimethylformamide

    DMSO dimethyl sulphoxide

    DOC sodium deoxycholate

    dsDNA double-stranded DNA

    DTE dithioerithriol

    DTT dithiothreitol

    ECL enhanced chemiluminescence

    EDTA ethylenediaminetetraacetic acid

    EEO electroendosmosis

    EGTA ethylene glycol-bis(?-aminoethylether)-N,N,N',N'-tetraacetic acid

    EKC electrokinetic chromatography

    ELISA enzyme-linked immunosorbent assay

    EMSA electrophoretic mobility shift assay

    EOF electroosmotic flow

    ER endoplasmic reticulum

    ESI electrospray ionization

    EST expressed sequence tag

    EUPA European Proteome Association

    FAB fast atom bombardment

    FACS fluorescence activated cell sorting

    FBS fetal bovine serum

    FCS fetal calf serum

    FIGE field inversion gel electrophoresis

    FITC fluorescein isothiocyanate

    FT Fourier transform

    FT-ICR Fourier transform-ion cyclotron resonance

    GC gas chromatography

    GIF graphic interchange format

    GRAVY grand average hydrophobicity

    GSH glutathione

    GST glutathione-S-transferase

    HE hematoxylin and eosin

    HEPES N-(2-hydroxyethyl)piperazine-2'-(2-ethanesulphonic acid)

    HPCE high-performance capillary electrophoresis

    HPLC high-performance liquid chromatography

    HRP horseradish peroxidase

    HSA human serum albumin

    HSP heat shock protein

    HTML hypertext mark-up language

    HUPO Human Proteome Organisation

    HVR hypervariable region

    ICAT isotop-coded affinity tag

    ICR ion cyclotron resonance

    id inside diameter

    IEF isoelectric focusing

    Ig immunoglobulin

    IMAC immobilized metal affinity capture

    IPG immobilized pH gradient

    IT ion trap

    iTRAQ isobaric tag for relative and absolute quantitation

    kbp kilobase pairs

    kDa kilodalton (molecular mass)

    LC liquid chromatography

    LED light-emitting diode

    LOD limit of detection

    LOQ limit of quantitation

    mAb monoclonal antibody

    MALDI-MS matrix-assisted laser-desorption ionization-mass spectrometry

    Mbp megabase

    MEKC micellar electrokinetic capillary chromatography

    MES 2-(N-morpholino)ethanesulphonic acid

    MHC major histocompatibility complex

    MOPS 3-(N-morpholino)propanesulphonic acid

    Mr relative molecular mass (dimensionless)

    MS mass spectrometry

    MS/MS tandem mass spectromet



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