List of contributors. Opening Address. Acknowledgements. Section I. Conceptual basis of VT. 1. Volume transmission as a key feature of information handling in the central nervous system. Possible new interpretative value of the Turing's B-type machine (L.F. Agnati, K. Fuxe).2. Integration of wiring transmission and volume transmission (F.E. Bloom).3. Ultrastructural evidence for diffuse transmission by monoamine and acetylcholine neurons of the central nervous system (L. Descarries, N. Mechawar).4. Comparative aspects of volume transmission, with sidelight on other forms of intercellular communication (R. Nieuwenhuys).Section II. Diffusion and extracellular space.5. Diffusion of molecules in brain extracellular space: theory and experiment (C. Nicholson, K.C. Chen, S. Hrabetová, L. Tao).6. Extracellular space diffusion and pathological states (E. Syková, T. Mazel, L. Vargová, I. Voríek, S. Prokopová). 7. Diffusion of radiolabeled dopamine, its metabolites and mannitol in the rat striatum studied by dual-probe microdialysis (J. Kehr, M. Höistad, K. Fuxe). Section III. Glia-neuronal signaling.8. Relationship between glia and the perineuronal nets of extracellular matrix in the rat cerebral cortex: importance for volume transmission in the brain (D. Viggiano, M.R. Celio)9. Glial influence on neuronal signaling (A. Chvátal, E. Syková)10. Glial modulation of neural excitability mediated by extracellular pH: a hypothesis revisited (B.R. Ransom).11. The astrocyte-mediated coupling between synaptic activity and energy metabolism operates through volume transmission (P.J. Magistretti, L. Pellerin).12. Metabolic trafficking between cells in nervous tissue (J.A. Coles, C. Véga, P. Marcaggi).13. Cell volume and water exchange in neural cells monitored by diffusion weighted 1H NMR spectroscopy (D. Leibfritz, J. Pfeuffer, U.Flögel, C. Meier, S. Bröer).Section IV. Monoamines and VT.14. Extrasynaptic distribution of monoamine transporters and receptors (V.M. Pickel).15. Distinct regional differences in dopamine-mediated volume transmission (M.E. Rice).16. Geometry and kinetics of dopaminergic transmission in the rat striatum and in mice lacking the dopamine transporter (F. Gonon, J.B. Burie, M. Jaber, M. Benoit-Marand, B. Dumartin, B.Bloch). 17. Evidence for the existence of pulses of dopamine in the extracellular space of the rat striatum (L.F. Agnati, M. Zoli, R. Ferrari, L. di Paola, C. Torri, K. Fuxe, I. Zini).18. Restoration of dopamine transmission in graft reinnervated striatum. Evidence for regulation of dopamine D2 receptor function in regions lacking dopamine (I. Strömberg, J. Kehr, K. Fuxe). 19. When it comes to communications between neurons, synapses are over-rated: insights from an animal model of Parkinsonism (M.J. Zigmond).Section V. The wider world of VT - from ions to peptides.20. GABAergic excitation and K+-mediated volume transmission in the hippocampus (J. Voipio, K. Kaila).21. Spillover and synaptic cross talk mediated by glutamate and GABA in the mammalian brain (D.M. Kullmann).22. Adenosine as a volume transmission signal. A feedback detector of neuronal activation (S. Ferr, K. Fuxe).23. Dynorphins are endogenous opioid peptides released from granule cells to act neurohumorly and inhibit excitatory neurotransmission in the hippocampus ( C. Chavkin).24. Neuropeptide spread in the brain and spinal cord (A.W. Duggan).25. Neuronal mechanisms of synaptic and network plasticity in the lamprey spinal cord (D. Parker, S. Grillner).26. Long distance signalling in volume transmission. Focus on clearance mechanisms (A. Jansson, A. Lippoldt, T. Mazel, T. Bartfai, S.-O. Ögren, E. Syková, L.F. Agnati, K. Fuxe).27. CSF signaling in physiology and behavior (M.Lehman, R. Silver).Section VI. Summary.28. Volume transmission in the year 2000 (C. Nicholson).