Table of ContentsFolic Acid:S.W. Bailey and J.E. Ayling, Total Chemical Synthesis of Chirally Pure (6S)-Tetrahydrofolic Acid.P.J. Bagley and J.Selhub, Analysis of Folates Using Combined Affinity and Ion Pair Chromatography.C.R. Santhosh-Kumar and J.F. Kolhouse, Molar Quantitation of Folates by Gas Chromatograph-Mass Spectrometry.D.W. Horne, Microbiological Assay of Folates in 96-Well Microtiter Plates.A.M. Molloy and J.M. Scott, Microbiological Assay for Serum, Plasma, and Red Cell Folate Using Cryopreserved Microtiter Plate Method.E.R. Werner, H. Wachter, and G. Werner-Felmayer, Determination of Tetrahydrobiopterin Biosynthetic Activities by High-Performance Liquid Chromatography with Fluorescence Detection.R.J. Leeming, Microtiter Plate Assay for Biopterin Using Cryopreserved Crithidia fasciculata. J. McPartlin and J.M. Scott, Identification and Assay of Folate Catabolites in Human Urine.V. Schirch, Enzymatic Determination of Folylpolyglutamate Pools.V. Schirch, Synthesis and Interconversion of Reduced Folylpolyglumates.A.L. Fitzhugh and R.K. Akee, Chemical Synthesis of (6S)-5-Formyl-5,6,7,8-tetrahydropteroyl-poly-gamma-L-glutamates.J. Fan and F.M. Huennekens, Biotin Derivatives of Folate Compounds: Synthesis and Utilization for Visualization and Affinity Purification of Folate Transport Proteins.C.M. Pfeiffer and J.F. Gregory, III, Preparation of Stable Isotopically Labeled Folates for in Vivo Investigation of Folate Absorption and Metabolism.S. Milstien, Interconversion of 6- and 7-Substituted Tetrahydropterins via Enzyme-Generated 4a-Hydroxytetrahydroprotein Intermediates.M. Hoshiga and K. Hatakeyama, Enzymatic Synthesis of 6R-U-14C Tetrahydrobiopterin from U-14CGTP.R.J. Cook, Use of 10-Formyldideazafolate as Substrate for Rat 10-Formyltetrahydrofolate.I. Atkinson, T. Garrow, A. Brenner, and B. Shane, Human Cytosolic Folylpoly-gamma-Glutamate Synthase.H.C. Imeson and E.A. Cossins, Folylpolyglutamate Synthase from Higher Plants.V. Schirch, Purification of Folate-Dependent Enzymes from Rabbit Liver.J. Jolivet, Human 5,10 Methenyltetrahydrofolate Synthetase.R.E. MacKenzie, Mitochondrial NAD-Dependent Methylenetetrahydrofolate Dehydrogenase-Methenyltetrahydrofolate Cyclohydrolase.D.R. Appling and M.G. West, Monofunctional NAD-Dependent 5,10-Methylenetetrahydrofolate Dehydrogenase from Saccharomyces cerevisiaeR. Banerjee, Z. Chen, and S. Gulati, Methionine Synthase from Pig Liver.J.T. Jarrett, K. Fluhr, C. Goulding, S. Huang, and R.G. Matthews, Purification and Assay of Cobalamin-Dependent Methionine Synthase from Escherichia coli. H. Zalkin, Formyltetrahydrofolate Hydrolase from Escherichi coli.D.R. Appling, E. Kastanos, L.B. Pasternack, and Y.Y. Woldman, Use of 13C Nuclear Magnetic Resonance to Evaluate Metabolic Flux through Folate One-Carbon Pools in Saccharomyces cerevisiae.Vitamin B12 and Cobalamins:C.B. Grisson and E. Natarajan, Use of Magnetic Field Effects to Study Coenzyme B12 Dependent Reactions.D.P. Sundin and R.H. Allen, Analysis of Cobalamin and Cobalamin Analogs by Gas Chromatography-Mass Spectrometry.M.M. Gordon, G. Russell-Jones, and D.H. Alpers, Expression of Functional Intrinsic Factor Using Recombinant Baculovirus.S.P. Rothenberg and E.V. Quadros, Quantitative Methods for Measurement of Transcobalamin II.L. Qian, E.V. Quadros and S.P. Rothenberg, Molecular Methods for Analysis and Expression of Transcobalamin II.S. Bose and B. Seetharam, Purification, Membrane Expression, and Interactions of Transcobalamin II Receptor.F. Watanabe and Y. Nakano, Purification and Characterization of Aquacobalamin Reductase from Euglena gracilis. F. Watanabe and Y. Nakano, Purification and Characterization of Aquacobalamin Reductases from Mammals.Heme:P.M. Shoolingin-Jordan, J.E. LeLean, and A.J. Lloyd, Continous Coupled Assay for 5-Aminolevulinate Synthase.P.M. Shoolingin-Jordan, M.J. Warren, and S.J. Awan, Dipyrromethane Cofactor Assembly of Porphobilinogen Deaminase: Formation of Apoenzyme and Preparation of Holoenzyme.P.M. Shoolingin-Jordan and R. Leadbeater, Coupled Assay for Uroporphyrinogen III Synthase.H.A. Dailey and T.A. Dailey, Expression and Purification of Mammalian 5-Aminolevulinate Synthase.H.A. Dailey and T.A. Dailey, Expression, Purification, and Characterization of Mammalian Protoporphyrinogen Oxidase.A.G. Roberts and G.H. Elder, Purification and Properties of Uroporphyrinogen Decarboxylase from Human Erythrocytes.T. Yoshinaga, Purification and Properties of Coproporphyrinogen III Oxidase from Bovine Liver.P. Labbe, Purification and Properties of Coproporphyrinogen III Oxidase from Yeast.V.M. Sellers and H.A. Daily, Expression, Purification and Characterization of Recombinant Mammalian Ferrochelatase.Miscellaneous Vitamins and Coenzymes:R.H. White, Structural Characterization of Modified Folates in Archaea.Methodologies Broadly Applicable to Vitamins and Coenzymes:J. Zempleni, Biokinetic Analysis of Vitamin Absorption and Disposition in Humans.M. Levine, S.C. Rumsey, Y. Wang, J. Park, O. Kwon, and N. Amano, In Situ Kinetics: An Approach to Recommended Intake of Vitamin C.Subject Index.Author Index.