Translation Initiation: Reconstituted Systems and Biophysical Methods

  • Jon Lorsch

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.
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Biochemists and researchers in related life science disciplines.


Book information

  • Published: October 2007
  • ISBN: 978-0-12-373969-8

Table of Contents

Chapter 1Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in BacteriaPohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. RodninaChapter 2Binding of mRNA to the Bacterial Translation Initiation ComplexSean M. Studer and Simpson JosephChapter 3Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methodsAttilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. PonChapter 4Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. SpremulliChapter 5In Vitro Studies of Archaeal Translational InitiationDario Benelli and Paola LondeiChapter 6Reconstitution of yeast translation initiationMichael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. LorschChapter 7Assembly and analysis of eukaryotic translation initiation complexesAndrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. PestovaChapter 8Reconstitution of Mammalian 48S Ribosomal Translation Initiation ComplexesRomit Majumdar, Jayanta Chaudhuri and Umadas Maitra Chapter 9Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analoguesAnna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard StolarskiChapter 10Biophysical studies of the translation initiation pathway using immobilised mRNA analoguesJohn E.G. McCarthy, Steven Marsden and Tobias von der HaarChapter 11Protection-Based Assays to Measure Amino Acyl-tRNABinding to Translation Initiation FactorsYves Mechulam*, Laurent Guillon, Laure Yatime, Sylvain Blanquet and Emmanuelle SchmittChapter 12NMR methods for studying protein-protein interactions involved in translation initiationAssen Marintchev, Dominique Frueh, and Gerhard WagnerƒxChapter 13Structural methods to study IRES FunctionJeffrey S. Kieft*, David A. Costantino, Megan E. Filbin, John Hammond, and Jennifer S. PfingstenChapter 14Biophysical and biochemical investigations of dsRNA-activated kinase PKR.Sean A. McKenna, Darrin A. Lindhout, Takashi Shimoike, and Joseph D. PuglisiChapter 15Expression and purification of recombinant wheat translation initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F and eIF5Laura K. Mayberry, Michael D. Dennis, M. Leah Allen, Kelley Ruud Nitka, Patricia A. Murphy, Lara Campbell and Karen S. BrowningChapter 16In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entryVictoria G. Kolupaeva, Sylvain de Breyne, Tatyana V. Pestova, Christopher U.T. Hellen