Translation Initiation: Extract Systems and Molecular Genetics

  • Jon Lorsch

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.
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Biochemists and researchers in related life science disciplines.


Book information

  • Published: October 2007
  • ISBN: 978-0-12-374191-2

Table of Contents

Chapter 1Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation InitiationWilliam C. Merrick and Diane Barth-BausChapter 2Studying translational control in Drosophila cell-free systemsFátima Gebauer and Matthias W. HentzeChapter 3Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational RegulationDaniel R. GallieChapter 4Viral translation and replication in vitroYuri V. Svitkin and Nahum SonenbergChapter 5A practical approach to isolate 48S complexes: Affinity purification and analysesNicolas Locker & Peter J. LukavskyChapter 6Yeast phenotypic assays on translational controlBumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura AsanoChapter 7Localization and characterization of protein-protein interaction sitesChingakham Ranjit Singh and Katsura Asano*Chapter 8In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking.Leos Valášek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. NielsenChapter 9Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5BByung-Sik Shin and Thomas E DeverChapter 10The use of fungal in vitro systems for studying translational regulationCheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs Chapter 11Investigating translation initiation using Drosophila molecular geneticsGritta Tettweiler and Paul LaskoChapter 12Analysis of RNA:protein interactions in vivo: Identification of RNA binding partners of nuclear factor 90Andrew M. Parrott, Melissa R. Walsh and Michael B. MathewsChapter 13Approaches for Analyzing the Differential Activities and Functions of eIF4E Family MembersRobert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary JagusChapter 14Tethered function assays: An adaptable approach to study RNA regulatory proteinsJeff Coller. Mary WickensChapter 15Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAsVincent P. Mauro, Stephen A. Chappell, and John Dresios