The Zebrafish: Genetics, Genomics and Informatics

Part I: Forward and Reverse Genetics

1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish - Wenbiao Chen

2. Advances in Tol2 Transposon Mutagenesis - Koichi Kawakami

3. OPEN Zinc Finger Nucleases - Keith Joung and Randy Peterson

4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish - Adam Amsterdam, Nancy Hopkins, and Shawn Burgess

5. Genetic Screens for Maternal-Effects Mutations - Francisco Pelegri and Mary Mullins

6. High-Throughput Target-Selected Gene Inactivation in Zebrafish - Derek Stemple

7. Suppressor Screens in Haploids - Xiaoying Bai

8. Transposons - Stephen Ekker

9. Caged MOs - James Chen

Part II: Transgenesis

1. Advanced Transgenesis with Cre Recombinase - Christian Mosimann

2. Transgenesis with phiC31 Recombinase - James Lister

3. Nuclear Transfer - Jose Cibelli and Dr. Siripattarapravat

Part III: The Zebrafish Genome and Mapping Technologies

1. The Zebrafish Genome Project: Sequence Analysis and Annotation- Derek Stemple

2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish - Charles Lee and Amanda Smith

3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome - John Postlethwait, Julian Catchen

4. The Zon Laboratory Guide to Positional Cloning in Zebrafish -Yi Zhou and Leonard Zon

Part IV: Informatics and Comparative Genomics

1. Data Warehouse ZFIN - Monte Westerfield and Judy Sprague

2. Epigenetics: Methylation - Brad Cairns

3. Chromatin Structure - Leonard Zon and Yi Zhou

4. ChipSeq - Brad Cairns

5. Micro RNAs - Graham Lieshke and Luke Pase

6. Microarrays - Yi Zhou

7. Sequence-Based Transcriptional Profiling - Shawn Burgess

8. Epigenetic Regulation of Zebrafish Development - Elke Ober and co-author

Part V: Infrastructure

1. Zebrafish Facilities for Small and Large Laboratories - Christian Lawrence

2. Genetic Backgrounds, Standard Lines, and their Husbandry - ZIRC, Zoltan