The Zebrafish: Genetics, Genomics and Informatics book cover

The Zebrafish: Genetics, Genomics and Informatics

This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.


Seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium

Included in series
Methods in Cell Biology

Hardbound, 528 Pages

Published: September 2011

Imprint: Academic Press

ISBN: 978-0-12-374814-0


  • Part I: Forward and Reverse Genetics

    1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish - Wenbiao Chen

    2. Advances in Tol2 Transposon Mutagenesis - Koichi Kawakami

    3. OPEN Zinc Finger Nucleases - Keith Joung and Randy Peterson

    4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish - Adam Amsterdam, Nancy Hopkins, and Shawn Burgess

    5. Genetic Screens for Maternal-Effects Mutations - Francisco Pelegri and Mary Mullins

    6. High-Throughput Target-Selected Gene Inactivation in Zebrafish - Derek Stemple

    7. Suppressor Screens in Haploids - Xiaoying Bai

    8. Transposons - Stephen Ekker

    9. Caged MOs - James Chen

    Part II: Transgenesis

    1. Advanced Transgenesis with Cre Recombinase - Christian Mosimann

    2. Transgenesis with phiC31 Recombinase - James Lister

    3. Nuclear Transfer - Jose Cibelli and Dr. Siripattarapravat

    Part III: The Zebrafish Genome and Mapping Technologies

    1. The Zebrafish Genome Project: Sequence Analysis and Annotation- Derek Stemple

    2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish - Charles Lee and Amanda Smith

    3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome - John Postlethwait, Julian Catchen

    4. The Zon Laboratory Guide to Positional Cloning in Zebrafish -Yi Zhou and Leonard Zon

    Part IV: Informatics and Comparative Genomics

    1. Data Warehouse ZFIN - Monte Westerfield and Judy Sprague

    2. Epigenetics: Methylation - Brad Cairns

    3. Chromatin Structure - Leonard Zon and Yi Zhou

    4. ChipSeq - Brad Cairns

    5. Micro RNAs - Graham Lieshke and Luke Pase

    6. Microarrays - Yi Zhou

    7. Sequence-Based Transcriptional Profiling - Shawn Burgess

    8. Epigenetic Regulation of Zebrafish Development - Elke Ober and co-author

    Part V: Infrastructure

    1. Zebrafish Facilities for Small and Large Laboratories - Christian Lawrence

    2. Genetic Backgrounds, Standard Lines, and their Husbandry - ZIRC, Zoltan


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