The Unfolded Protein Response and Cellular Stress, Part B book cover

The Unfolded Protein Response and Cellular Stress, Part B

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.


Researchers and students in cell, molecular and developmental biology

Included in series
Methods in Enzymology

Hardbound, 432 Pages

Published: February 2011

Imprint: Academic Press

ISBN: 978-0-12-385114-7


  • Section I: Measuring and Modifying the UPR

    1. Methods for investigating the UPR in filamentous fungi
    2. Thomas Guillemette, Arthur F.J. Ram, Neuza D.S.P. Carvalho, Aymeric Joubert, Philippe Simoneau, David B. Archer

    3. Assays for detecting the unfolded protein response
    4. Karen Cawley, Shane Deegan, Afshin Samali, Sanjeev Gupta

    5. Analysis of the role of nerve growth factor in promoting cell survival during endoplasmic reticulum stress in PC12 cells
    6. Koji Shimoke , Harue Sasaya, and Toshihiko Ikeuchi

    7. Measuring ER stress and the unfolded protein response using mammalian tissue culture system
    8. Christine M. Oslowski and Fumihiko Urano

    9. Real-Time Monitoring of Er Stress in Living Cells And Animals Using Estrap Assay
    10. Masanori Kitamura and Nobuhiko Hiramatsu

    11. HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells
    12. Huiping Zhou

    13. Dexamethasone induction of a heat-stress response
    14. José A. Guerrero, Vicente Vicente, Javier Corral

    15. Detecting and Quantitating Physiological Endoplasmic Reticulum Stress
    16. Ling Qi, Liu Yang and Hui Chen

      Section II: Molecular Components of the UPR

    17. PI 3-Kinase Regulatory Subunits as Regulators of the Unfolded Protein Response
    18. Jonathon N. Winnay and C. Ronald Kahn

    19. The Emerging Role of Histone Deacetylases (HDACs) in UPR regulation
    20. Soumen Kahali, Bhaswati Sarcar & Prakash Chinnaiyan

    21. Immunohistochemical detection of Activating Transcription Factor 3, a hub of the cellular adaptive-response network
    22. Tsonwin Hai, Swati Jalgaonkar, Christopher C. Wolford, and Xin Yin

    23. Experimental approaches for elucidation of stress-sensing mechanisms of the Ire1 family proteins
    24. Daisuke Oikawa and Yukio Kimata

    25. Measurement and Modification of the Expression Level of the Chaperone Protein and Signaling Regulator GRP78/BiP in Mammalian Cells
    26. Wan-Ting Chen and Amy S. Lee

    27. The Endoplasmic Reticulum associated degradation and disulfide reductase ERdj5
    28. Ryo Ushioda and Kazuhiro Nagata

    29. Structural Insight into the Protective Role of P58(IPK) during Unfolded Protein Response
    30. Jiahui Tao, Bingdong Sha

    31. Principles of IRE1 modulation using chemical tools
    32. Kenneth P. K. Lee and Frank Sicheri

    33. Methods to study stromal-cell derived factor 2 in the context of ER stress and the unfolded protein response in Arabidopsis thaliana
    34. Andrea Schott & Sabine Strahl

    35. Nitrosative stress-induced S-glutathionylation of protein disulfide isomerase
    36. Joachim D. Uys, Ying Xiong, and Danyelle M. Townsend

    37. Methods for Analyzing eIF2 Kinases and Translational Control in the Unfolded Protein Response

    Brian F. Teske, Thomas D. Baird, and Ronald C. Wek


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