Tetrahymena Thermophila, 62

  • David Asai, Purdue University, West Lafayette, Indiana, U.S.A.
    • James Forney, Purdue University, West Lafayette, Indiana, U.S.A.
      • Leslie Wilson, University of California, Santa Barbara, U.S.A.
        • Paul Matsudaira, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.


        Cell biologists, developmental biologists, geneticists, and molecular biologists.


Book information

  • Published: August 1999
  • ISBN: 978-0-12-544164-3

Table of Contents

D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History of Tetrahymena thermophila.
J. Frankel, Cell Biology of Tetrahymena thermophila.
K.M. Karrer,Tetrahymena Genetics: Two Nuclei are Better Than One.

E. Orias, E.P. Hamilton, and J.D. Orias, Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance.
P.J. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage.
E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants.
P.J. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis.
S.L. Allen, Isolation of Micronuclear and Macronuclear DNA.
E. Orias and E.P. Hamilton, Genetically Sorting a Collection of Tetrahymena Mutants.
E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes.
D.G. Pennock, Selection of Motility Mutants.
K.R. Stuart and E.S. Cole, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques.
E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy.
W. Dentler, Fixation of Tetrahymena Cells for Electron Microscopy.
R.H. Gavin, J.G. Hoey, and J.A. Garcés, Immunoelectron Microscopy of Tetrahymena.

Cell Biology:
A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion in Tetrahymena thermophila.
T.M. Hennessey and H.G. Kuruvilla, Electrophysiology of Tetrahymena.
E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization of in Vivo Modified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase.
C. Autexier, Tetrahymena Telomerase Activity, Purification, and Reconstruction.
E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Studying the Telomerase RNA in Tetrahymena.
T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein from Tetrahymena Cilia.
N.E. Williams Preparation of Cytoskeletal Fractions from Tetrahymena thermophila.
N.E. Williams, Immunoprecipitation Procedures.
O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe, Tetrahymena Calcium-Binding Proteins, TCBP-25 and TCBP-23.

Manipulating Genes:
D.L. Chalker, J.G. Ward, C. Randolph, and M.-C. Yao, Microinjection of Tetrahymena thermophila.
J. Gaertig and G. Kapler, Transient and Stable DNA Transformation of Tetrahymena thermophila by Electroporation.
P.J. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei.
B. Hai, J. Gaertig, an M.A. Gorovsky, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in Tetrahymena.
Q. Fan, R. Sweeney, and M.-C. Yao, Creation and Use of Antisense Ribosomes in Tetrahymena thermophila.
L. Yu and M.A. Gorovsky, Protein Tagging in Tetrahymena.

Appendix I: Genetic Nomenclature Rules for Tetrahymena thermophila.
Appendix II: Codon Usage in Tetrahymena thermophila.
Chapter References.
Volumes in Series.