Synthetic Biology, Part A
Methods for Part/Device Characterization and Chassis Engineering
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.
Included in series
Methods in Enzymology
Methods in Enzymology
Hardbound, 714 Pages
Published: May 2011
Imprint: Academic Press
ISBN: 978-0-12-385075-1
Contents
- Section I. Measuring and Engineering Central Dogma Processes
- Sequence-specificity and Energy Landscapes of DNA-binding Molecules Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari
- Promoter Reliability in Modular Transcriptional Networks Rajat Anand, Navneet Rai and Mukund Thattai
- The Analysis of ChIP-seq Data Wenxiu Ma and Wing Hung Wong
- Using DNA Microarrays to Assay Part Function Virgil A. Rhodius and Carol A. Gross
- Orthogonal gene expression in Escherichia coli Wenlin An and Jason Chin
- Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos Section II. Device and System Design, Optimization, and Debugging
- Design and Connection of Robust Genetic Circuits Ron Weiss
- Engineering RNAi circuits Yaakov Benenson
- From SELEX to cell: Dual selections for synthetic riboswitches Joy Sinha, Shana Topp, and Justin P. Gallivan
- Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You
- De novo design and construction of an inducible gene expression system in mammalian cells Maria Karlsson, Wilfried Weber and Martin Fussenegger
- BioBuilding: Using banana-scented bacteria to teach synthetic biology James Dixon and Natalie Kuldell Section III. Device Measurement, Optimization, and Debugging
- Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics Gürol Süel
- Microfluidics for synthetic biology: From design to execution M. S. Ferry, I. A. Razinkov, J. Hasty
- Plate-based assays for light-regulated gene expression systems Jeffrey J. Tabor
- Spatiotemporal control of small GTPases with light using the LOV domain Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn
- Light Control of Plasma Membrane Recruitment using the Phy-PIF system Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner
- Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes Ed Boyden Section IV. Devices for Metabolic Engineering
- Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding John E. Dueber and Weston R. Whitaker
- A Synthetic Iterative Pathway for ketoacid elongation C. R. Shen, J. C. Liao Section V. Expanding Chasses
- Synthetic Biology in Streptomyces bacteria Marnix Medema, Rainer Breitling and Eriko Takano
- Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra
- Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty
- Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter Lindblad
- Developing a Synthetic Signal Transduction System in Plants Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. Medford
- Lentiviral Vectors to Study Stochastic Noise in Gene Expression
