Sphingolipid Metabolism and Cell Signaling, Part A, 311

  • Alfred Merrill, Jr., Emory University, Atlanta, Georgia, U.S.A.
    • Yusuf Hannun, Medical University of South Carolina, Charleston, U.S.A.
      • John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
        • Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, U.S.A.


        Biochemists, molecular biologists, cell biologists, biomedical researchers, microbiologists, and developmental biologists.


Book information

  • Published: October 1999
  • ISBN: 978-0-12-182212-5


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Table of Contents

Sphingolipid Metabolism: Biosynthesis: R.C. Dickson, R.L. Lester, and M. Marek-Nagiec, Serine Palmitoyltransferase. M.M. Grilley and J. Y. Takemoto, Assay of the Saccharomyces cerevisiae Dihydrosphingosine C-4 Hydorxylase. B. Caligan, K. Peters. J. Ou, E. Wang, and A.H. Merrill, Jr., Ceramide Synthase. H. Schulze, C. Michel, and G. van Echten-Deckert, Dihydroceramide Desaturase. M.N. Nikolava-Karakashian, Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine. J.A. Shayman and A. Abe, Glucosylceramide Synthase: Assay and Properties. P. Marks, Y. Kamisaka, and R.E. Pagano, Methods for Studying Glucosylceramide Synthase. H. Sprong, G. van Meer, and P. van der Sluijs, UDP-Galactose: Ceramide Galactosyltransferase. S. Chatterjee, Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction. G. Pohlentz, C. Kaes, and K. Sandhoff, In Vitro Assays for Enzymes of Ganglioside Synthesis. F.B. Jungalwala, P. Chatturvedi, D.S. Newburg, and M.R. Natowicz, Analyses of Sulfatides and Enzymes of Sulfatide Metabolism. J.A. Shayman and A. Abe, 1-O-Acyleceramide Synthase. T-C. Lee, N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase. A.S. Fischl, Y. Liu, A. Browdy, and A.E. Cremesti, Inositolphosphoryl Ceramide Synthase from Yeast. D.V. Lynch, Enzymes of Shingolipid Metabolism in Plants.

Turnover: S. Lansmann, O. Bartelsen, and K. Sandhoff, Purification and Characterization of Recombinant Human Acid Sphingomyelinase. B. Liu and Y.A. Hannun, Purification of Rat Brain Membrane Neutral Sphingomyelinase. B. Liu and Y.A. Hannun, Sphingolmyelinase Assay using Radiolabeled Substrates. A.G. Barbone, A.C. Jackson, D.M. Ritchie, and D.C. Argentieri, Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening. D.F. Hassler, R. M. Laethem, and G.K. Smith, A High Through-put Sphingomyelinase Assay. R.T. Dobrowsky and V.G. Rao, Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes. M.N. Nikolova-Karakashian and A.H. Merrill, Jr.,Ceramidases. T.Linke, S. Lansman, and K. Sandhoff, Purification of Acid Ceramidase from Human Placenta. S. Bajjalieh and R. Batchelor, Ceramide Kinase. A.Olivera, K.D. Barlow, and S. Spiegel, Assaying Sphingosine Kinase Activity. L.M. Obeid, Sphingosine 1-Phosphate Phosphatase. D.N. Brindley, J. Xu, R. Jasinska, and D.W. Waggoner, Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities. P. Van Veldhover, Sphingosine 1-Phosphate Lyase. W. Bierfreund, T. Kolter, and K. Sandhoff, Sphingolipid Hydrolases and Activator Proteins. R-D. Duan and A. Nilsson, Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract. M. Basu, P. Kelly, M. Girzadas, Z. Li, and S. Basu, Properties of Animal Ceramide Glycanases. M. Ito, K. Kita, T. Kurita, N. Sueyoshi, and H. Izu, Enzymatic N-Deacylation of Shingolipids.

Genetic Approaches: S. Ichikawa and Y. Hirabayashi, Genetic Approaches for Studies of Glycolipid Synthetic Enzymes. N. Chung and L.M. Obeid, Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling.

Inhibitors and Sphingolipid Biosynthesis: S.M. Mandala and G. H. Harris, Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin. R.T. Riley and R.D. Plattner, Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii. F. Meredith, Isolation and Characterization of Fumonisins. J.A. Shayman, L. Lee, A. Abe, and L. Shu, Inhibitors of Glucosylceramide Synthase.

Chemical and Enzymatic Syntheses: C. Cufman and D.C. Liotta, Synthesis of Sphingosine and Sphingoid Bases. K-H. Jung and R.K. Schmidt, Synthesis of Sphingosine, Radiolabeled Sphiongosine, 4-Methyl-cis-Sphingosine and 1-Amino Derivatives of Sphingosine via their Azido Derivatives. P.K. Koskinen and A.M.P. Koskinen, Total Synthesis of Sphingosine and its Analogs. A. Bielawska, Y.A. Hannun, and A. Szulc, Radiolabeled Sphingolipids, Part I. Radiolabeling in the Sphingolipid Backbone A. Bielawska and Y.A. Hannun, Radiolabeled Sphingolipids, Part II. Preparation of Radiolabeled Ceramides and Phospholsphingolipids. A. Bielawska, Z. Szulc, and Y.A. Hannun, Radiolabeled Sphingolipids, Part III. Synthesis of Key Precursors. A.S. Bushnev and D.C. Liotta, Practical Synthesis of N-Palmitoylsphingomyelin and N-Palmitoyldihydrosphingomyelin. M. Kiso and H. Ishida, Synthesis of Glycolipids, with a Focus on GM3, and Sulfatide Analogs. F. Knoll, T. Kolter, and K. Sandhoff, Sphingolipid Photoaffinity Labels. G. Schwarzmann, Synthesis and Characterization of Metabolically Stable Sphingolipids. J. Fanitni, Synthetic Soluble Analogs of Glycolipids for Studies of Virus Glycolipid Interactions. S. Sonnino, V. Chigorno, and G. Tettamanti, Preparation of Radioactive Gangliosides, 3H or 14C Isotopically Labeled at the Oligosaccharide or Ceramide Moieties. L. Riboni, P. Viani, and G. Tettamanti, Estimating Sphingolipid Metabolism and Trafficking in Cultured Cells Using Radiolabeled Compounds. M. Ito, S. Mitsutake, M. Tani, and K. Kita, Enzymatic Synthesis of 14C Ceramide, 14 C Glycosphingolipids, and W-Aminoceramide.