Small GTPases in Disease, Part A

Edited by

  • W. Balch, The Scripps Research Institute, La Jolla, CA, USA
  • Channing Der, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA
  • Alan Hall, University College of London, U.K.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: This volume is the first of two planned volumes on the topic of small GTPases and their role in disease.
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Researchers in academics and industry studying biochemistry.


Book information

  • Published: April 2008
  • ISBN: 978-0-12-373968-1

Table of Contents

Chapter 1Rab1b silencing using small interfering RNA for analysis of disease specific functionDarren M. Hutt, William E. BalchChapter 2Rab8-optineurin-myosin VI: Analysis of interactions and functions in the secretory pathwayMargarita V. Chibalina, Rhys C. Roberts, Susan D. Arden, John Kendrick-Jones and Folma BussChapter 3Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate specific antigen in prostate carcinoma cellsSergio D. CatzChapter 4In vitro assays to characterize inhibitors of the activation of small G proteins by their guanine nucleotide exchange factorsJean-Christophe Zeeh, Bruno Antonny, Jacqueline Cherfils, Mahel ZeghoufChapter 5Analysis of small GTPase function in trypanosomesMark C. Field, David Horn and Mark CarringtonChapter 6Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosisTom Kirchhausen, Eric Macia, Henry E. PelishChapter 7Identification and verification of Sro7p as an effector of the Sec4p rab GTPaseBianka Grosshans1 and Peter NovickChapter 8Characterization of Rab18, a Lipid Droplet Associated Small GTPase Sally Martin, Robert G. Parton Chapter 9Analysis on the Emerging Role of of Rab3 GTPase-activating Protein in Warburg Micro and Martsolf SyndromeAyuko Sakane, Jun Miyoshi, Yoshimi Takai, Takuya SasakiChapter 10Identification and Characterization of JRAB/MICAL-L2, a Junctional Rab13-binding ProteinNoriyuki Nishimura and Takuya SasakiChapter 11A SMAP Gene Family Encoding ARF GTPase-activating Proteins and Its Implication in Membrane TraffickingKENJI TANABE, SHUNSUKE KON, NOBUYUKI ICHIJO, TOMO FUNAKI, WAKA NATSUME, TOSHIO WATANABE, and MASANOBU SATAKEChapter 12In vitro and in vivo analysis of neurotrophin-3 activation of Arf6 and Rac-1.Pedro F. Esteban, Paola Caprari, Hye-Young Yoon, Paul A. Randazzo & Lino TessarolloChapter 13Methods for analysis of rab27a/munc13-4 in secretory lysosome release in haematopoietic cellsPeter van der Sluijs, Maaike Neeft, Thijs van Vlijmen, Edo Elstak, and Marnix WiefferChapter 14Analysis and expression of Rab38 in Oculocutaneous Lung DiseaseKazuhiro Osanai, Dennis R. VoelkerChapter 15Analysis of Rab1 function in cardiomyocyte growth Guangyu Wu, Catalin M. Filipeanu, Fuguo ZhouChapter 16Regulation of the Trafficking and Function of G Protein-coupled Receptors by Rab1 GTPase in CardiomyoctyesGuangyu WuChapter 17Regulation of endosome dynamics by Rab5 and its Huntingtin-HAP40 effector complex in physiological versus pathological conditionsArun Pal, Fedor Severin, Sebastian Höpfner and Marino ZeriaChapter 18BIOCHEMICAL AND BIOPHYSICAL ANALYSES OF RAS MODIFICATION BY UBIQUITINSonia Terrillon and Dafna Bar-Sagi Chapter 19Biochemical Characterization of Novel Germline BRAF and MEK Mutations in Cardio-facio-cutaneous Syndrome Pablo Rodriguez-Viciana and Katherine A. RauenChapter 20Biochemical and biological characterization of tumor-associated mutations of p110aAdam Denley, Marco Gymnopolous, Jonatha R. Hart, Hao Jiang, Li Zhao, Peter K. Vogt Chapter 21Characterization of the Rheb-mTOR signaling pathway in mammalian cells: Constitutive active mutants of Rheb and mTORTatsuhiro Sato, Akiko Umetsu, Fuyuhiko TamanoiChapter 22Characterization of RalB-Sec5-TBK1 Function in Human OncogenesisYuchen Chien and Michael A. WhiteChapter 23High-throughput lung cancer cell line screening for genotype-correlated sensitivity to an EGFR kinase inhibitorUltan McDermott, Sreenath V. Sharma, Jeffrey SettlemanChapter 24In vitro signalling by MAPK and NFkB pathyways inhibited by Yersinia YopJSohini Mukherjee, Kim Orth Chapter 25Biological and biochemical characterization of anthrax lethal factor, a proteolytic inhibitor of MEK signaling pathways. Jennifer L. Bromberg-White and Nicholas S. DuesberyChapter 26Genetic analyses of the role of RCE1 in RAS membrane association and transformationMartin O. Bergo, Annika M. Wahlstrom, Loren G. Fong, Stephen G. YoungChapter 27Characterization of Kras Mediated Pancreatic Tumorigenesis in ZebrafishJon M. Davison, Seung Woo Park, Jerry M. Rhee, Steven D. LeachChapter 28Genetic Modeling of Ras-Induced Human RhabdomyosarcomaCorinne M. Linardic, Christopher M. Counter