Separation Methods in Drug Synthesis and Purification
- K. Valkó, Physical Sciences, GlaxoWellcome Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK.
The new technologies in drug research such as combinatorial chemistry, high throughput screening, and robotics have made it possible to synthesise millions of molecules for thousands of screens. This volume presents the theory and applications of a wide range of separation methods used in the early stages of drug synthesis, purification and characterisation.
The topics covered are high performance liquid chromatography, capillary electrophoresis, capillary electro-chromatography, enantioseparations, preparative chromatography, and thin-layer chromatography. The separation methods used for the determination of physical-chemical properties together with the quantitative structure–retention relationship studies are also reviewed. The academic contributors provide a detailed description of the basic principles while the industrial contributors give real life examples of their application. The book not only reviews the major achievements in the field but also contains methods which have never been published before but are frequently used in pharmaceutical research.
To avoid the lengthy process of method development for every compound going through quality control, generic methods have been developed using fast gradient reversed-phase chromatography. Generic methods developed for high-throughput quality information generation are presented. The most important factors influencing column selection and gradient conditions are compared with the information gained, time and cost.
Similarly, generic methods can be developed for capillary electrophoresis in drug analysis. The recently emerged separation technique, capillary electro-chromatography, has received great attention in the pharmaceutical industry and is discussed in this volume. The various hyphenated analytical separation techniques widely applied in combinatorial assays for the identification of molecules available only in very small quantities are also presented.
The most stimulating section of this volume is the presentation of completely automated methods and technologies that can handle the analysis of hundreds of compounds weekly. A unique feature of the book is a demonstration of how separation sciences can contribute to the high throughput physical chemical profiling of the compounds, and how it can help in quantitative studies of structure–activity relationships. The optimisation strategies for process scale chromatography and process chromatography are also reviewed.
For chemists and pharmaceutical chemists in pharmaceutical companies, research development analytical laboratories of government and industry, and universities.