Separation and Purification Techniques in Biotechnology


  • Frederick Dechow, Mediquest, Bothell, WA, USA

This book describes separation and purification techniquesùadsorption, ion exchange and liquid chromatography on solid supportsùused for fermentation and biochemical feedstreams. Emphasis is placed on basic sorption theory, laboratory evaluation techniques, sorptive materials and their characteristics, scale-up of laboratory techniques, and their industrial applications. Each chapter contains specific examples illustrating the use of purification techniques in biotechnology processes.
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Engineers and scientists in the fermentation and biochemicals industries.


Book information

  • Published: December 1989
  • ISBN: 978-0-8155-1197-7

Table of Contents

1. Introduction 1.1 Fermentation Broth 1.2 Recovery Unit Operations 1.3 Recovery Processes 1.4 References2. Adsorption 2.1 Introduction 2.2 Adsorption Theory 2.3 Types of Adsorbents 2.4 Laboratory Evaluation of Adsorbents 2.5 Adsorbent Operations 2.6 Process Considerations 2.7 Adsorption Applications 2.8 References3. Ion Exchange 3.1 Introduction 3.2 Theory 3.3 Ion Exchange Materials and Their Properties 3.4 Laboratory Evaluation Resin 3.5 Ion Exchange Processes 3.6 Ion Exchange Operations 3.7 Process Considerations 3.8 Biotechnology Applications Appendix 3.9 References4. Column Chromatography Processes 4.1 Introduction 4.2 Classifications of Chromatography 4.3 Chromatographic Materials 4.4 Theory 4.5 Laboratory Techniques 4.6 Scale-Up Procedures 4.7 Industrial Systems 4.8 Chromatography Applications References5. Affinity Chromatography 5.1 Introduction 5.2 Theory of Affinity Chromatography 5.3 Affinity Chromatography Materials 5.4 Laboratory Practices 5.5 Scale-Up of Laboratory Procedures 5.6 Affinity Chromatography Operations 5.7 Affinity Chromatography Applications 5.8 ReferencesIndex