Table of ContentsBasal Transcription and Regulation of Transcription: Transcription Factors, Activators, and Repressors: H.E. Choy, A Complete Ribosome-Free in Vitro Transcription System Responsive to Extract of Escherichiacoli. A. Miller, M. Choi, M.A. Glucksmann-Kuis, X. Dai, and L.B. Rothman-Denes, Single-Stranded DNA-Binding Proteins as Transcriptional Activators. S.E. Hasnain, S. Habib, A. Jain, S. Burma, and B. Mukherjee, Host Factor with Single-Stranded DNA-Binding Activity Involved in Transcription from Baculovirus Polyhedrin Promoter. N. Goosen, P. van Ulsen, L. Zulianello, and P. van de Putte, Transcription Activiation by Histone-like Protein IHF. D.M. Hinton, R. March-Amegadzie, J.S. Gerber, and M. Sharma, Bacteriophage T4 Middle Transcription System: T4-Modified RNA Polymerase, AsiA (Sigma-70 Binding Protein), and Transcriptional Activator MotA. H. Ge, E. Martinez, C.-M. Chiang, and R.G. Roeder, Activator-Dependent Transcription by Mammalian RNA Polymerase II: In Vitro Reconstitution with General Transcription Factors and Cofactors. E. Maldonado, R. Drapkin, and D. Reinberg, Purification of Human RNA Polymerase II and General Transcription Factors. W.-C. Yeh, J. Hou, and S.L. McKnight, Purification and Characterization of Gene-Specific Transcription Factors from Rat Liver: c/EBP, GABP, and IL-4 Stat. M. Zhong, J. Wisniewski, M. Fritsch, M. Mizuguchi, A. Orosz, P. Jedlicka, and C. Wu, Purification of Heat ShockTranscription Factor of Drosophila. J. Greenblatt and C.J. Ingles, Interaction between Acidic Transcriptional Activation Domains of Activator Protein VP16 Herpes Simplex Virus and TRIID. M. Carey, D.Tantin, T. Chi, R. Hori, and S. Pyo, Biochemical Mechanism of Transcriptional Activation by Ga14-VP16. R. Valcarcel and H.G. Stunnenberg, Retinoid-Dependent in Vitro Transcription. D. Thanos and T. Maniatis, In Vitro Assembly of Enhancer Complexes. G. Stegar, J. Ham, and M. Yaniv, E2 Proteins: Modulators of Papillomavirus Transcription and Replication. Chromatin as Templates for Transcription: L.P. ONeill and B.M. Turner, Immunoprecipitation of Chromatin. F. Thoma, Mapping of Nucleosome Positions. M.P.Kladde and R.T. Simpson, Chromatin Structure Mapping in Vivo Using Methyltransferases. A.E. Sippel, H. Saueressig, M.C. Huber, H.C. Hoefer, A. Stief, U. Borgmeyer, and C. Bonifer, Identification of cis-Acting Elements as DNase I Hypersensitive Sites in Lysozyme Gene Chromatin. V.M. Studitsky, D.J. Clarke, and G. Felsenfeld, Preparation of Nucleosomal Templates for Transcription in Vitro. K. Ura and A.P. Wolffe, Reconstruction of Transcriptionally Active and Silent Chromatin. C. Ueguchi and T. Mizuno, Purification of H-NS Protein and Its Regulatory Effect on Transcription in Vitro. R.T. Utley, T.A.O. Hughes, L.-J. Juan, J. Cite, C.C. Adams, and J.L. Workman, In Vitro Analysis of Transcription Factor Binding to Nucleosomes and Nucleosome Disruption/Displacement. T. Tsukiyama and C. Wu, Purification of GAGA Factor of Drosophila and Its Role in Nucleosome Disruption. M.C. Barton and B.M. Emerson, Regulated Gene Expression in Reconstituted Chromatin and Synthetic Nuclei of Xenopus. Transcription Elongation and Termination: Factors and Complexes: S. Borukhov and A. Goldfarb, Purification and Assay of Escherichia coli Transcript Cleavage Factors GreA and GreB. M. Kashlev, E. Nudler, K.Severinov, S. Borukhov, N. Komissarova, and A. Goldfarb, His-Tagged Polymerase of Escherichia coli and Transcription in Solid Phase. R. Landick, D. Wang, and C.L. Chan, Quantitative Analysis of Transcriptional Pausing by Escherichia coli Polymerase: his Leader Pause Site as Paradigm. W. Nowatzke, L. Richardson, and J.P. Richardson, Purification of Transcription Termination Factor Rho from Escherichia coli and Micrococcus luteus. A. Das, S. Barek, B. Ghosh, and W. Whalen, Immunoprinting: A Technique to Study Dynamic Protein¿Nucleic Acid Interactions within the Transcription Elongation Complex. A. Das, M. Pal, J.G. Meno, W.N. Lalen, K. Wolska, R. Crossley, W. Rees, P.H. von Hippel, N. Constantino, D. Court, M.Mazzulla, A.S. Altieri, R.A. Byrd, S. Chattopadhyay, J. de Vito, and B. Ghosh, Components of a Multiprotein-RNA Complex That Controls Transcription Elongation in Escherichia coli Phage Lambda. M.M. Hanna, Photochemical Cross-linking Analysis of Protein¿Nucleic Acid Interactions in Escherichia coli Transcription Complexes from the Lambda PR1 Promoter. A.M. Edwards and C.M. Kane, Identifying Regulators of Transcript Elongation in Eukaryotes. A. Pause, T. Aso, W.M. Linehan, J.W. Conaway, R.C. Conaway, and R.D. Klausner, Interaction of the VHL Tumor Suppressor Gene Product with Elongin. Specialized Technique: T.D. Schneider, Reading of DNA Sequence Logos: Prediction of Major Groove Binding by Information Theory. D. Chatterjee and V. Gopal, Fluorescence Spectroscopy Analysis of Active and Regulatory Sties of RNA Polymerase. M. Hsieh and M. Brenowitz, Quantitative Kinetics Footprinting: Protein¿DNA Association Reactions. T. Heyduk, Y.Ma, H. Tang, and R.H. Ebright, Fluorescence Anisotropy: Assay for Protein¿DNA and Protein¿Protein Interaction. K.A. Sharif, J. Luo, and J.S. Krakow, Epitope Mapping of Monoclonal Antibodies Directed against Subunits of RNA Polymerase ofEscherichia coli. N.E. Thompson and R.R. Burgess, Immunoaffinity Purification of Wheat Germ RNA Polymerase II and Transcription Factors Using Polyol-Responsive Monoclonal Antibodies. Author Index. Subject Index.