RNA helicases book cover

RNA helicases

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases.

Audience
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 584 Pages

Published: June 2012

Imprint: Academic Press

ISBN: 978-0-12-396546-2

Contents

    1. Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy
      Andrea A. Putnam & Eckhard Jankowsky

    2. Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods
      Michael J. Bradley & Enrique M. De La Cruz

    3. Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases
      David D. Hackney

    4. Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer
      Alexandra Z. Andreou & Dagmar Klostermeier

    5. RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases
      Jeffrey P. Potratz & Rick Russell

    6. Molecular Mechanics of RNA Translocases
      Steve C. Ding & Anna Marie Pyle

    7. Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping
      Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat & Marc Boudvillain

    8. Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase
      Nathan D. Thomsen & James M. Berger

    9. Structural Analysis of RNA Helicases with Small-angle X-ray Scattering
      Manja A. Behrens, Yangzi He, Cristiano L. P. Oliveira, Gregers R. Andersen, Jan Skov Pedersen & Klaus H. Nielsen

    10. Analysis of Co-factor Effects on RNA Helicases
      Crystal Young & Katrin Karbstein

    11. Analysis of DEAD-box Proteins in mRNA Export
      Ben Montpetit, Markus A. Seeliger & Karsten Weis

    12. Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay
      Francesca Fiorini, Fabien Bonneau & Hervé Le Hir

    13. Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)
      Markus T. Bohnsack, David Tollervey & Sander Granneman

    14. In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly
      David C. Rawling & Susan J. Baserga

    15. Analysis of RNA Helicases in P-bodies and Stress Granules
      Angela Hilliker

    16. DEAD box RNA Helicases as Transcription Co-factors
      Frances V. Fuller-Pace & Samantha M. Nicol

    17. DEAD-box RNA Helicases in Gram-positive RNA-decay
      Peter Redder & Patrick Linder

    18. RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches
      George W. Owttrim

    19. Determination of Host RNA Helicases Activity in Viral Replication
      Amit Sharma & Kathleen Boris-Lawrie

    20. Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A
      Regina Cencic, Gabriela Galicia-Vázquez & Jerry Pelletier

    21. Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase
      Alicia M. Hanson, John J. Hernandez, William R. Shadrick, & David N. Frick

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