RNA helicases book cover

RNA helicases

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases.

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 584 Pages

Published: June 2012

Imprint: Academic Press

ISBN: 978-0-12-396546-2


    1. Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy
    2. Andrea A. Putnam & Eckhard Jankowsky

    3. Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods
    4. Michael J. Bradley & Enrique M. De La Cruz

    5. Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases
    6. David D. Hackney

    7. Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer
    8. Alexandra Z. Andreou & Dagmar Klostermeier

    9. RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases
    10. Jeffrey P. Potratz & Rick Russell

    11. Molecular Mechanics of RNA Translocases
    12. Steve C. Ding & Anna Marie Pyle

    13. Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping
    14. Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat & Marc Boudvillain

    15. Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase
    16. Nathan D. Thomsen & James M. Berger

    17. Structural Analysis of RNA Helicases with Small-angle X-ray Scattering
    18. Manja A. Behrens, Yangzi He, Cristiano L. P. Oliveira, Gregers R. Andersen, Jan Skov Pedersen & Klaus H. Nielsen

    19. Analysis of Co-factor Effects on RNA Helicases
    20. Crystal Young & Katrin Karbstein

    21. Analysis of DEAD-box Proteins in mRNA Export
    22. Ben Montpetit, Markus A. Seeliger & Karsten Weis

    23. Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay
    24. Francesca Fiorini, Fabien Bonneau & Hervé Le Hir

    25. Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)
    26. Markus T. Bohnsack, David Tollervey & Sander Granneman

    27. In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly
    28. David C. Rawling & Susan J. Baserga

    29. Analysis of RNA Helicases in P-bodies and Stress Granules
    30. Angela Hilliker

    31. DEAD box RNA Helicases as Transcription Co-factors
    32. Frances V. Fuller-Pace & Samantha M. Nicol

    33. DEAD-box RNA Helicases in Gram-positive RNA-decay
    34. Peter Redder & Patrick Linder

    35. RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches
    36. George W. Owttrim

    37. Determination of Host RNA Helicases Activity in Viral Replication
    38. Amit Sharma & Kathleen Boris-Lawrie

    39. Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A
    40. Regina Cencic, Gabriela Galicia-Vázquez & Jerry Pelletier

    41. Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase

    Alicia M. Hanson, John J. Hernandez, William R. Shadrick, & David N. Frick


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