RNA EditingEdited by
- Jonatha Gott, Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA
Biochemists, molecular biologists, computational and systems biologists, plant biologists, and molecular parasitologists working in the fields of RNA editing and modification.
Methods in Enzymology
Hardbound, 592 Pages
Published: August 2007
Imprint: Academic Press
- Trypanosome U insertion/deletion.Chapter 1: Isolation and Compositional Analysis of Trypanosomatid Editosomes. Chapter 2: U insertion/deletion editing activities.Chapter 3: RNA Editing Uridylyltransferases of Trypanosomes. Chapter 4: Isolation of RNA binding proteins involved in insertion/deletion editingChapter 5: RNA-protein Interactions in Assembled Editing Complexes in Trypanosomes.Chapter 6: Strategies of Kinetoplastid Cryptogene Discovery and Analysis.Physarum insertion editing.Chapter 7: RNA editing in Physarum mitochondria: assays and biochemical approaches.Chapter 8: Computational approaches to insertional RNA editing. Chapter 9: Evolution of RNA Editing Sites in the Mitochondrial Small Subunit rRNA of the Myxomycota.5' deletion/insertion editing of tRNAs. Chapter 10: Analysis of 5´- or 3´-Terminal tRNA Editing: Mitochondrial 5´ tRNA Editing in Acanthamoeba castellanii as the Exemplar.A to I editing.Chapter 11: Comparative genomic and bioinformatic approaches for the identification of new adenosine to inosine substrates.Chapter 12: Genetic approaches to studing adenosine to inosine RNA editing. Chapter 13: A method to find sites of selective adenosine deamination.Chapter 14 : Purification and assay of ADAR activity.Chapter 15: Large Scale Overexpression and Purification of ADARs from S. cerevisiae for Biophysical and Biochemical Studies.Chapter 16: Mouse models to elucidate the functional roles of A-to-I editing.Chapter 17: Probing Adenosine to Inosine Editing Reactions using RNA containing Nucleoside Analogs.Nuclear C to U editing.Chapter 18: Measuring Editing Activity and Identifying C to U mRNA Editing Factors in Cells and Biochemical Isolates.Chapter 19: Mouse Models as Tools to Explore C to U RNA Editing.Editing in plant organellesChapter 20: RNA editing in plant mitochondria: assays and biochemical approaches. Chapter 21: Assay of Editing of Exogenous RNAs in Chloroplast Extracts of Arabidopsis, Maize, Pea, and Tobacco.Chapter 22: In organello gene expression and RNA editing studies by electroporation-mediated transformation of isolated plant mitochondria.Chapter 23: Transformation of the Plastid Genome to Study RNA Editing.