Regenerative Biology and Medicine


  • David Stocum, Department of Biology, Indiana University-Purdue University Indianapolis, USA

The purpose of the book is to bring together in one place the different facets of regenerative biology and medicine while providing the reader with an overview of the basic and clinically-oriented research that is being done. Not only does the content cover a plethora tissues and systems, it also includes information about the developmental plasticity of adult stem cells and the regeneration of appendages.As part of its balanced presentation, Regenerative Biology and Medicine does address the biological/bioethical issues and challanges involved in the new and exciting field of regenerative biology and medicine.
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Researchers and students in biology, chemistry, and bioengineering; medical students; academic and clinical physicians; and research investigators


Book information

  • Published: August 2006
  • ISBN: 978-0-12-369371-6


"...a terrific reference for anyone toying with the idea of moving into this field. The text offers an enormous breath of coverage of different systems currently under investigation, and Stocum provides a central theme that pits regenerative ability against fibrosis as a way to conceptually untangle the limited regenerative capacity of humans...Stocum has erected an umbrella large enough for scientists of diverse backgrounds to initiate conceptual cross-talk between those actively involved in the biology of regeneration and those targeting its clinical application." - Ken Muneoka, Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana “…of tremendous importance to researchers and clinicians working in the fields of regeneration and stem cell biology.” - Jonathan Henry, University of Illinois at Urbana-Champaign “This text integrates information from cell and developmental biology with regenerative biology, tissue engineering and clinical practice. The breadth and detail of this intellectual landscape are derived from the author’s long commitment to the problems of regeneration, and help to make this volume a landmark publication for regenerative medicine.” - Jeremy Brockes, University College London “This book is a superb in-depth analysis of the existing potential of the tissues of the body to replace cells and tissues and how to promote this ability for the sake of repair….Every institute should have it as their background reading and mission statement. I can't recommend it highly enough, it has been long awaited.” - Malcolm Maden, King’s College London “…wonderful and comprehensive, providing readers with the basis of regeneration biology and medicine. The author emphasizes the necessity of understanding the biology of regeneration for a true appreciation of its practice in clinical medicine.” - Katsutoshi Yoshizato, University of Hiroshima

Table of Contents

Chapter 1: An Overview of Regenerative Biology and Medicine IntroductionA Brief History of Regenerative Biology and MedicineThe Biology of Regeneration1. Regeneration Takes Place at all Levels of Biological Organization2. Mechanisms of Regeneration at the Tissue Level3. Epimorphosis and Morphallaxis4. Evolutionary Significance of Regeneration and FibrosisStrategies of Regenerative Medicine1. Cell Transplantation2. Bioartificial Tissues3. Induction of Regeneration In SituSummary ReferencesChapter 2: Repair of Skin Wounds by FibrosisIntroductionStructure of Adult Mammalian SkinThe Effect of Wound Type and Extent on Dermal Repair Phases of Repair in Excisional Wounds1. Hemostasis and Clot Formation2. Inflammation3. Structural Repair4. The Role of Wound Contraction in Dermal RepairMolecular Comparison of Wounded vs. Unwounded SkinFetal Skin Heals Without Scarring 1. Cellular and ECM Differences Between Fetal and Adult Wound Healing2. Fetal Wounds have a Minimal Inflammatory ResponseSummary References Chapter 3: Regeneration of Epidermal TissuesIntroductionRegeneration of Epidermis and Hair 1. Maintenance Regeneration2. Injury-Induced Regeneration of the IFE Regeneration of NailsRegeneration of Dental TissuesRegeneration of the Lens1. Structure of the Eye and Lens2. Cellular Events of Lens Regeneration in the Newt Eye 3. Transcription Factors Regulating Lens Regeneration4. Signals Invlved in Lens Regeneration5. Mammals Have Some Capacity for Lens RegenerationRegeneration of the CorneaSummary ReferencesChapter 4: Regenerative Medicine of Skin, Hair and Dental TissuesIntroductionRepair of Skin 1. Acceleration of Acute Wound Repair by Topically Applied Agents2. Acceleration of Repair in Chronic Wounds by Topically Applied Agents3. Keratinocyte Transplants4. Bioartificial Skin Equivalents5. Acellular Dermal Regeneration TemplatesUse of Acellular Templates for Abdominal Wall RepairStimulation of Hair RegenerationRepair of Teeth and PeridontiumCorneal RegenerationSummary ReferencesChapter 5: Regeneration of Neural Tissues IntroductionAxon Regeneration1. Axons of Mammalian Spinal (Peripheral) Nerves2. Axons of Mammalian Spinal Cord 3, Axons of Amphibian Spinal Cord 4. Axons of Amphibian and Fish Optic Nerve Maintenance Regeneration of Neurons in the Mammalian CNS1. Discovery of Neural Stem Cells 2. Regeneration of Olfactory Nerve and Bulb Neurons3. Regeneration of Hippocampal Neurons4. Regeneration of Cortical Neurons 5. The CNS Neural Stem Cell MicronicheInjury-Induced CNS Regeneration 1. Avian and Mammalian Auditory Sensory Neurons2. Regeneration of Neurons in the Mammalian Brain3. Regeneration of the Retina 4. Regeneration of the Spinal Cord in Amputated Amphibian and Lizard TailsSummary ReferencesChapter 6: Regenerative Medicine of Neural Tissues IntroductionTherapies for Injured Peripheral Nerve Therapies for Injured Spinal Cord 1. Pharmaceutical Therapies2. Regeneration Templates 3. Cell Transplant Therapies4. Bioartificial Spinal Cord5. Rehabilitation Programs and Synaptic Plasticity6. Combinatorial Approaches May Bring Better SuccessTherapies for Neurodegenerative Diseases1. Demyelinating Disorders2. Parkinson¡¦s Disease3. Huntington¡¦s Disease4. Alzheimer¡¦s Disease5. Amyotrophic Lateral SclerosisSummary ReferencesChapter 7: Regeneration of Digestive, Respiratory and Urogenital TissuesIntroduction Intestinal Epithelium1. Structure of the Intestinal Tract 2. Regeneration of the Villous Epithelium3. Regeneration of Transected IntestineLiver1. Structure and Function of the Mammalian Liver 2. Regeneration by Compensatory Hyperplasia3. Injury-Induced Regeneration via Stem Cells4. Heterogeneity of Hepatocyte Size and Growth PotentialPancreas1. Structure and Function of the Pancreas2. Regeneration of the Pancreas3. Origin of Regenerated ƒÒ-CellsAlveolar Epithelium of the Lung1. Structure of the Respiratory System2. Regeneration of Alveolar EpitheliumKidney and Urinary System 1. Structure of the Kidney and Urinary System2. Regeneration of Kidney Tubule EpitheliumGonadsProstate TissueSummary ReferencesChapter 8: Regenerative Medicine of Digestive, Respiratory and Urinary TissuesIntroductionRegenerative Therapies for the Liver1. Hepatocyte Transplants2. Extracorporeal Liver Assist DevicesRegenerative Therapies for the Pancreas 1. Cell Transplants2. Suppression of Autoimmunity and Regeneration From Remaining ƒÒ-Cells3. Bioartificial Pancreas4. Gene Therapy to Induce Islet NeogenesisRegenerative Therapies for the Esophagus and Intestine1. Esophagus2. IntestineRegenerative Therapies for the Respiratory System1. Trachea2. LungRegenerative Therapies for the Urinary System 1. Nephrons2. Urinary Conduit TissueSummary ReferencesChapter 9: Regeneration of Musculoskeletal Tissues IntroductionRegeneration of Skeletal Muscle1. Structure of Skeletal Muscle2. Satellite Cells are the Source of Regenerated Muscle3. Cellular and Molecular Events of Skeletal Muscle Regeneration 4. The Activation and Proliferation of SCs is Regulated by a Variety of Signaling Molecules5. Tension and Innervation are Required for Normal Muscle RegenerationRegeneration of Bone1. Structure of Bone2. Maintenance Regeneration of Bone3. Injury-Induced Regeneration of BoneRepair of Articular CartilageRepair of Tendon and Ligament1. Structure of Tendons and Ligaments2. Repair of Tendons and LigamentsSummary ReferencesChapter 10: Regenerative Medicine of Musculoskeletal TissuesIntroductionRegenerative Therapies for Muscle1. Muscular Dystrophy2. Bioartificial MuscleRegenerative Therapies for Meniscus and Articular Cartilage1. Meniscus2. Articular CartilageRegenerative Therapies for Bone 1. Electromagnetic Field Stimulation2. Ilizarov Distraction Technique for Lengthening Bones3. Induction of Bone Regeneration by Osteogenic Factors and Acellular Templates4. Cell Transplants and Bioartificial Bone5. The Long Bones of Salamanders Can Regenerate When RemovedRegenerative Therapies for Tendon and Ligament1. Tendon2. LigamentSummary ReferencesChapter 11: Regeneration of Hematopoietic and Cardiovascular TissuesIntroductionRegeneration of Hematopoietic Cells1. Composition of Adult Blood2. Hematopoietic Stem Cells of the Bone Marrow3. Regulation of LT-HSC Activation and ProliferationRegeneration of Blood Vessels1. Development and Structure of the Vascular System2. Blood Vessel Regeneration in Wounded TissueRegeneration of Cardiac Muscle1. Structure of Cardiac Muscle2. Mammalian Cardiac Muscle Does Not Regenerate, but Harbors Adult Stem Cells 3. Amphibian and Zebrafish Cardiac Muscle Regenerates When InjuredSummaryReferencesChapter 12: Regenerative Medicine of Hematopoietic and Cardiovascular TissuesIntroductionTherapies for Hematopoietic Disorders1. Myeloablative Therapy2. Non-Myeloablative Therapy 3. The Future of Hematopoietic Transplants4. Gene Therapy for Genetic Hematopoietic DiseasesTherapies for Blood Vessel Regeneration 1. Stem Cell Transplants 2. Acellular Regeneration Templates 3. Bioartificial Blood VesselsTherapies for Protection and Regeneration of the Infarcted Myocardium1. Transplants of Bone Marrow Cells2. Satellite Cell Transplants3. Cardiomyocyte Transplants4. Soluble Factors and Myocardial Regeneration5. Bioartificial Cardiac MuscleSummary ReferencesChapter 13: Regenerative Medicine: Developmental Plasticity of Adult Stem Cells IntroductionAssays to Test Developmental Plasticity Experimental Results1. Neural Stem Cells2. Satellite Cells3. Liver Oval Cells4. Dental Pulp Cells5. Unfractionated Bone Marrow and HSCs6. MSCs of the Bone Marrow and Related MSCsMany Reports of Lineage Conversion are Due to Fusion With Host CellsAdult Stem Cell Pluripotency: Fact or Fiction?Might Organisms Contain ESC-Like Stem Cells in Their Tissues?Effects of Aging on Number and Developmental Status of Regeneration-Competent CellsSummary ReferencesChapter 14: Regeneration of AppendagesIntroductionAmphibian Limb Regeneration1. Events of Limb Regeneration 2. Origin of the Blastema 3. Mechanism of Blastema Formation4. Epidermal and Neural Requirements for Survival and Proliferation of Blastema Cells5. Developmental Plasticity of Blastema Cells in Regenerating Amphibian Limbs6. Spatial Organization of Tissue Patterns in the Regenerating Limb 7. Stimulation of Frog Limb Regeneration8. Comparative Analysis of Gene Activity in Regeneration-Competent vs. Regeneration-Deficient LimbsRegeneration of Amphibian JawsAppendage Regeneration in Mammals1. Regeneration of Ear Tissue2. Regeneration of Deer Antlers3. Regeneration of Mouse and Human Digit Tips4. Stimulation of Digit and Limb Regeneration in Mice and RatsSummary ReferencesChapter 15: Research Issues in Regenerative Medicine IntroductionBiological Issues and Challenges. 1. Cell Sources for Transplantation and Bioartificial Tissue Construction 2. Expansion and Directed Differentiation of Regeneration-Competent Cells xiii3. Challenges for Bioartificial Tissue Construction4. The Challenge of Immunorejection5. Challenges for the Chemical Induction of Regeneration6. The Challenge of Curing DiseaseBioethical Issues and Challenges1. The Derivation of Designer Human ESCs by SCNT 2. The Controversy Over Human ESC Research 3. Resolving an Unresolvable Dilemma4. The Specter of Interspecies GraftingConcluding RemarksSummary References