Table of ContentsC.D. O'Connor, J.N.B. Walker, and J.R. Saunders, RsrII: A Restriction Endonuclease with a Heptanucleotide Recognition Sequence. B.-Q. Qiang and I. Schildkraut, NotI and SfiI: Restricton Endonucleases with Octanucleotide Recognition Sequences. M. McClelland, Site-Specific Cleavage of DNA at 8-, 9-, and 10-bp Sequences. M. Nelson and M. McClelland, Purification and Assay of Type II DNA Methylases. M. Nelson and I. Schildkraut, The Use of DNA Methylases to Alter the Apparent Recognition Specificities of Restriction Endonucleases. Rapid Methods for DNA Sequence Analysis. A.T. Bankier, K.M. Weston, and B.G. Barrell, Random Cloning and Sequencing by the M13/Dideoxynucleotide Chain Termination Method. G.-F. Hong, The Use of DNase I, Buffer Gradient Gel, and 35S Label for DNA Sequencing. L. Dente and R. Cortese, pEMBL: A New Family of Single-Stranded Plasmids for Sequencing DNA. T.K. Misra, DNA Sequencing: A New Strategy to Create Ordered Deletions, Modified M13 Vector, and Improved Reaction Conditions for Sequencing by Dideoxy Chain Termination Method. R. Zagursky and K. Baumeister, Construction and Use of pBR322 Plasmids That Yield Single-Stranded DNA for Sequencing. S. Henikoff, Unidirectional Digestion with Exonuclease III in DNA Sequence Analysis. S. Labeit, H. Lehrach, and R.S. Goody, DNA Sequencing Using *ga-Thiodeoxynucleotides. A. Ahmed, Use of Transposon-Promoted Deletions in DNA Sequence Analysis. R.M.K. Dale and A. Arrow, A Rapid Single-Stranded Cloning, Sequencing, Insertion, and Deletion Strategy. Z.-g. Peng and R. Wu, A New and Simple Rapid Method for Sequencing DNA. G. Volckaert, A Systematic Approach to Chemical DNA Sequencing by Subcloning in pGV451 and Derived Vectors. F.M. Pohl and S. Beck, Direct Transfer Electrophoresis Used for DNA Sequencing. L.M. Smith, R.J. Kaiser, J.Z. Sanders, and L.E. Hood, The Synthesis and Use of Fluorescent Oligonucleotides in DNA Sequence Analysis. A. Rosenthal, R. Jung, and H.-D. Hunger, Solid-Phase Methods for Sequencing of Oligodeoxynucleotides and DNA. Miscellaneous Methods. K.B. Mullis and F.A. Faloona, Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction. D. Koshland and P. Hieter, Visual Assay for Chromosome Ploidy. S. Nishimura, N. Shindo-Okada, and P.F. Crain, Isolation of Amino Acid-Specific tRNA by High-Performance Liquid Chromatography. Y. Kuchino, N. Hanyu, and S. Nishimura, Analysis of Modified Nucleosides and Nucleotide Sequence of tRNA. P.A. Krieg and D.A. Melton, In Vitro RNA Synthesis with SP6 RNA Polymerase. H. Bujard, R. Gentz, M. Lanzer, D. Stueber, M. Mueller, I. Ibrahimi, M.-T. Haeuptle, and B. Dobberstein, A T5 Promoter-Based Transcription*b1Translation System for the Analysis of Proteins in Vitro and in Vivo. G.L. Igloi and H. K"ssel, Use of Boronate-Containing Gels for Electrophoretic Analysis of Both Ends of RNA Molecules. C.L. Smith and C.R. Cantor, Purification, Specific Fragmentation, and Separation of Large DNA Molecules. G.F. Carle and M.V. Olson, Orthogonal-Field-Alternation Gel Electrophoresis. L.S. Lerman and K. Silverstein, Computational Simulation of DNA Melting and Its Application to Denaturing Gradient Gel Electrophoresis. R.M. Myers, T. Maniatis, and L.S. Lerman, Detection and Localization of Single Base Changes by Denaturing Gradient Gel Electrophoresis. J. Berman, S. Eisenberg, and B.-K. Tye, An Agarose Gel Electrophoresis Assay for the Detection of DNA-Binding Activities in Yeast Cell Extracts. T.D. Tullius, B.A. Dombroski, M.E.A. Churchill, and L. Kam, Hydroxyl Radical Footprinting: A High-Resolution Method for Mapping Protein*b1DNA Contacts. D.E. Hill, A.R. Oliphant, and K. Struhl, Mutagenesis with Degenerate Oligonucleotides: An Efficient Method for Saturating a Defined DNA Region with Base Pair Substitutions. A.R. Oliphant and K. Struhl, The Use of Random-Sequence Oligonucleotides for Determining Consensus Sequences. F.I. Lewitter and W.P. Rindone, Computer Programs for Analyzing DNA and Protein Sequences. Author Index. Subject Index.