Recombinant DNA, Part D, 153

Volume 153: Recombitant Dna Part D

  • Ray Wu, Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.
    • Lawrence Grossman, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.
      • John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
        • Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, U.S.A.


        Biochemists, molecular biologists, microbiologists, geneticists, analytical chemists, clinical chemists; industrial researchers working on protein products; plant breeders and plant pathologists.


Book information

  • Published: December 1987
  • ISBN: 978-0-12-182054-1


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Table of Contents

Vectors for Cloning DNA:Production of Single-Stranded Plasmid DNA.pKUN, Vectors for the Separate Production of Both DNA Strands of Recombinant Plasmids.Restriction Site Bank Vectors for Cloning in Gram-Negative Bacteria and Yeast.Plasmids for the Selection and Analysis of Prokaryotic Promoters.A *gl DNA Protocol Based on Purification of Phage on DEAE-Cellulose.Double cos Site Vectors: Simplified Cosmid Cloning.Improved in Vitro Packaging of *gl DNA.*gl Phage Vectors--Embl Series.Plasmid and Phage Vectors for Gene Cloning and Analysis in Streptomyces.Cosmid Shuttle Vectors for Cloning and Analysis of Streptomyces DNA.Host-Vector Systems for Gene Cloning in Cyanobacteria.Genetic Engineering of the Cyanobacterial Chromosome.Conjugal Transfer of Plasmids to Cyanobacteria.Site-Directed Chromosomal Rearrangements in Yeast.Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers.Vectors for Cloning in Plant Cells.Binary Ti Vectors for Plant Transformation and Promoter Analysis.Detection for Monocot Transformation via Agrobacterium tumefaciens.Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation.Uptake of DNA and RNA into Cells Mediated by Electroporation.Electroporation of DNA and RNA into Plant Protoplasts.Cloning Vectors of Mitochondrial Origin for Eukaryotes.Vectors for Expression of Cloned Genes:Short Homopeptide Leader Sequences Enhanced Production of Human Proinsulin in Escherichia coli.Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone by Cathepsin C Cleavage.Expression of Eukaryotic Genes in Escherichia coli with a Synthetic Two-Cistron System.Expression of Heterologous Unfused Protein in Eshcerichia coli.Directing Ribosomes to a Single mRNA Species: A Method to Study Ribosomal RNA Mutations and Their Effects on Translation of a Single Messenger in Escherichia coli.New Expression Vectors for Identifying and Testing Signal Structures for Initiation and Termination of Transcription.Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli.Expression Plasmid Containing the *gl PL Promoter and cI857 Repressor.Expression and Secretion of Foreign Proteins in Escherichia coli.Engineering for Protein Secretion in Gram-Positive Bacteria. Expression and Secretion Vectors for Yeast.Vaccinia Virus as an Expression Vector.Author Index.Subject Index.