Proteomic Profiling and Analytical Chemistry
The CrossroadsEdited by
- Pawel Ciborowski
- Jerzy Silberring
Proteomic Profiling and Analytical Chemistry helps scientists without a strong background in analytical chemistry to understand basic analytical principles and apply them to proteomics profiling. In most proteomic profiling experiments, liquid chromatography is used; this method is also used widely in analytical chemistry. This book bridges the gap between overly specialized courses and books in mass spectrometry, proteomics and analytical chemistry. It also helps researchers with an analytical chemistry background to break into the proteomics field.
Proteomic Profiling and Analytical Chemistry focuses on practical applications for proteomic research helping readers to design better experiments and to more easily interpret, analyze and validate the resulting data. Experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more are all covered in detail.
analytical chemists, mass spectrometrists, researchers in proteomics, molecular biologists, biotechnologists, and pharmaceutical scientists.
Hardbound, 264 Pages
Published: January 2013
"Because of the heavy emphasis on sample preparation and protein separation, the book is incredibly useful as an overview of these methodologies, specifically pointing out steps and precautions to take for optimal sample qualityâ¦This book fills a void in proteomic literature and provides a starting point for novices to understand sample preparation and pitfalls to avoid, without delving into details of how a mass spectrometer functions."-- Doody.com,November 8, 2013 "In this concise guide for researchers, technicians, and students, Ciborowski and Silberring document how to use analytical techniques to ensure precision and low rates of false positives in the fast-growing field of proteomics research."-- Reference & Research Book News,October 2013
Chapter 1: Introduction
Chapter 2: Biomolecules
Chapter 3: Fundamental Strategies of Protein and Peptide Sample Preparation
Chapter 4: Protein Extraction and Precipitation
Chapter 5: Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample
Chapter 6: Gel Electrophoresis
Chapter 7: Quantitative Measurements in Proteomics
Chapter 8: Proteomic Database Search and Analytical Quantification
Chapter 9: Microarrays and Dynamics of Fluorescent Dyes
Chapter 10: Design and Statistical Analysis of Mass-Spectrometry Based Quantitative Proteomics Data
Chapter 11: Principles of Analytical Validation
Chapter 12: Validation in Proteomics and Regulatory Affairs