Proteomic Profiling and Analytical Chemistry book cover

Proteomic Profiling and Analytical Chemistry

The Crossroads

Proteomic Profiling and Analytical Chemistry helps scientists without a strong background in analytical chemistry to understand basic analytical principles and apply them to proteomics profiling. In most proteomic profiling experiments, liquid chromatography is used; this method is also used widely in analytical chemistry. This book bridges the gap between overly specialized courses and books in mass spectrometry, proteomics and analytical chemistry. It also helps researchers with an analytical chemistry background to break into the proteomics field.

Proteomic Profiling and Analytical Chemistry focuses on practical applications for proteomic research helping readers to design better experiments and to more easily interpret, analyze and validate the resulting data. Experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more are all covered in detail.

Audience

analytical chemists, mass spectrometrists, researchers in proteomics, molecular biologists, biotechnologists, and pharmaceutical scientists.

Hardbound, 264 Pages

Published: January 2013

Imprint: Elsevier

ISBN: 978-0-444-59378-8

Reviews

  • "Because of the heavy emphasis on sample preparation and protein separation, the book is incredibly useful as an overview of these methodologies, specifically pointing out steps and precautions to take for optimal sample quality…This book fills a void in proteomic literature and provides a starting point for novices to understand sample preparation and pitfalls to avoid, without delving into details of how a mass spectrometer functions."--Doody.com, November 8, 2013
    "In this concise guide for researchers, technicians, and students, Ciborowski and Silberring document how to use analytical techniques to ensure precision and low rates of false positives in the fast-growing field of proteomics research."--Reference & Research Book News, October 2013


Contents

  • Chapter 1: Introduction
    Chapter 2: Biomolecules
    Chapter 3: Fundamental Strategies of Protein and Peptide Sample Preparation
    Chapter 4: Protein Extraction and Precipitation
    Chapter 5: Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample
    Chapter 6: Gel Electrophoresis
    Chapter 7: Quantitative Measurements in Proteomics
    Chapter 8: Proteomic Database Search and Analytical Quantification
    Chapter 9: Microarrays and Dynamics of Fluorescent Dyes
    Chapter 10: Design and Statistical Analysis of Mass-Spectrometry Based Quantitative Proteomics Data
    Chapter 11: Principles of Analytical Validation
    Chapter 12: Validation in Proteomics and Regulatory Affairs

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