Protein Phosphorylation, Part A: Protein Kinases: Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression

Volume 200: Protein Phosphorulation Part A

Edited by

  • Tony Hunter, The Salk Institute, La Jolla, California, USA
  • Bartholomew Sefton, The Salk Institute, San Diego, California, U.S.A.


  • John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
  • Melvin Simon, The Salk Institute, La Jolla, CA, USA

This volume provides a compilation of recent methods for studying protein phosphorylation.
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Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.


Book information

  • Published: July 1991
  • ISBN: 978-0-12-182101-2


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Table of Contents

Classification of Protein Kinases and Phosphorylation Site Sequences:T. Hunter, Protein Kinase Classification.S.K. Hanks and A.M. Quinn, Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members.R.B. Pearson and B.E. Kemp, Protein Kinase Phosphorylation Site Sequences and Consensus Specificity Motifs: Tabulations.Assays of Protein Kinases:C.V.C. Glover and C.D. Allis, Enzyme Activity Dot Blots for Assaying Protein Kinases.S.-L. Li, D. Sahal, and Y. Fujita-Yamaguchi, Solid-Phase Protein-Tyrosine Kinase Assays.G. Rijksen, B.A. van Oirschit, and G.E.J. Staal, Nonradioactive Assays of Protein-Tyrosine Kinase Activity Using Antiphosphotyrosine Antibodies.E. Racker, Use of Synthetic Amino Acid Polymers for Assay of Protein-Tyrosine and Protein-Serine Kinases.E. Racker and P.C. Sen, Assay of Phosphorylation of Small Substrates and of Synthetic Random Polymers That Interact Chemically with Adenosine 5prime-Triphosphate.J.E. Casnellie, Assay of Protein Kinases Using Peptides with Basic Residues for Phosphocellulose Binding.B.E. Kemp and R.B. Pearson, Design and Use of Peptide Substrates for Protein Kinases.D.R. Marshak and D. Carroll, Synthetic Peptide Substrates for Casein Kinase II. Purification of Protein Kinases: General Methods:S. Ferrari and G. Thomas, Micro and Macro Purification Methods for Protein Kinases.J.R. Woodgett, Use of Synthetic Peptides Mimicking Phosphorylation Sites for Affinity Purification of Protein-Serine Kinases.P. Jeno and G. Thomas, Affinity Purification of Protein Kinases Using Adenosine 5prime-Triphosphate, Amino Acid, and Peptide Analogs.Bacterial Protein Kinases:J.F. Hess, R.B. Bourret, and M.I. Simon, Phosphorylation Assays for Proteins of Two-Component Regulatory System Controlling Chemotaxis in Escherichia coli.K.A. Borkovich and M.I. Simon, Coupling of Receptor Function to Phosphate-Transfer Reactions in Bacterial Chemotaxis.J.-C. Cortay, D. N~aaegre, and A.J. Cozzone, Analyzing Protein Phosphorylation in Prokaryotes.Protein-Serine Kinases:K. Ogita, Y. Ono, U. Kikkawa, and Y. Nishizuka, Expression, Separation, and Assay of Protein Kinase C Subspecies.P.J. Parker and R.M. Marais, Purification of Protein Kinase C Isotypes from7078v, cont.Bovine Brain:K.-P. Huang and F.L. Huang, Purification and Analysis of Protein Kinase C Isozymes.E. Erikson, J.L. Maller, and R.L. Erikson, Xenopus Ribosomal Protein S6 Kinase II.H.A. Lane and G. Thomas, Purification and Properties of Mitogen-Activated S6 Kinase from Rat Liver and 3T3 Cells.J.-C. Labbe, J.-C. Cavadore, and M. Dore~aae, M-Phase-Specific CDC2 Kinase: Preparation from Starfish Oocytes and Properties.L.J. Cisek and J.L. Corden, Purification of Protein Kinases That Phosphorylate Repetitive Carboxyl-Terminal Domain of Eukaryotic RNA Polymerase II.T.C. Chambers and T.A. Langan, Purification and Properties of Growth-Associated H1 Histone Kinase.S.H. Francis, L. Wolfe, and J.D. Corbin, Purification of Type I~ga and Type I~gb Isozymes and Proteolyzed Type I~gb Monomeric Enzyme of Guanosine Cyclic Monophosphate-Dependent Protein Kinase from Bovine Aorta.T.W. Sturgill, L.B. Ray, N.G. Anderson, and A.K. Erickson, Purification of Mitogen-Activated Protein Kinase from Epidermal Growth Factor-Treated 3T3-L1 Fibroblasts.J.L. Benovic, Purification and Characterization of ~gb-Adrenergic Receptor Kinase.D. Carling, P.R. Clarke, and D.G. Hardie, Adenosine Monophosphate-Activated Protein Kinase: Hydroxymethylylutaryl-CoA Reductase Kinase.Protein-Tyrosine Kinases:L. R~adonnstrand and C.-H. Heldin, Purification of Platelet-Derived Growth Factor ~gb-Receptor from Porcine Uterus.J. Hsuan and P. Yaish, Affinity Purification of Active Epidermal Growth Factor Receptor Using Monoclonal Antibodies.Protein-Histidine Kinases:Y.-F. Wei and H.R. Matthews, Identification of Phosphohistidine in Proteins and Purification of Protein Histidine Kinases.Renaturation of Protein Kinases:J.E. Hutchcroft, M. Anostario, Jr., M.L. Harrison, and R.L. Geahlen, Renaturation and Assay of Protein Kinases after Electrophoresis in Sodium Dodecyl Sulfate-Polyacrylamide Gels.J.L. Celenza and M. Carlson, Renaturation of Protein Kinase Activity on Protein Blots.J.E. Ferrell, Jr., and G.S. Martin, Assessing Activities of Blotted Protein Kinases.S.M. Kee, C.-J. Yuan, and D.J. Graves, High-Performance Liquid Chromatographic Separation and Renaturation of Protein Kinase Subunits: Application to Catalytic Subunit of Phosphorylase Kinase.Antibodies against Protein Kinases:A. Kishimoto, N. Saito, K. Ogita, and U. Kikkawa, Preparation and Use of Protein Kinase C Subspecies-Specific Antipeptide Antibodies for Immunostaining.F.L. Huang, Y. Yoshida, and K.-P. Huang, Preparation, Characterization, and Use of Isozyme-Specific Antiprotein Kinase C Antibodies.G.M. Clinton and N.A. Brown, Generation and Use of Antipeptide Antibodies Directed against Catalytic Domain of Protein Kinases.Affinity Labeling, Chemical Probing, and Crystallization of Protein Kinases:B.E. Haley, Nucleotide Photoaffinity Labeling of Protein Kinase Subunits.J.A. Buechler, J. Toner-Webb, and S.S. Taylor, Carbodiimides as Probes for Protein Kinase Structure and Function.W.T. Miller, Peptide-Based Affinity Labeling of Adenosine Cyclic Monophosphate-Dependent Protein Kinase.J. Zheng, D.R. Knighton, J. Parello, S.S. Taylor, and J.M. Sowadski, Crystallization of Catalytic Subunit Adenosine Cyclic Monophosphate-Dependent Protein Kinase.Cloning of Genes Encoding Protein Kinases:S.K. Hanks and R.A. Lindberg, Use of Degenerate Oligonucleotide Probes to Identify Clones That Encode Protein Kinases.A.F. Wilks, Cloning Members of Protein Tyrosine Kinase Family Using Polymerase Chain Reaction.M.H. Kraus and S.A. Aaronson, Detection and Isolation of Protein-Tyrosine Kinase Genes Employing Reduced Stringency Hybridization.R.A. Lindberg and E.B. Pasquale, Isolation of cDNA Clones That Encode Active Protein-Tyrosine Kinases Using Antibodies against Phosphotyrosine.J.R. Woodgett, cDNA Cloning and Properties of Glycogen Synthase Kinase-3.Expression and Analysis of Protein Kinases Using cDNA Clones:W. Yonemoto, M.M. McGlone, L.W. Slice, and S.S. Taylor, Prokaryotic Expression of Catalytic Subunit of Adenosine Cyclic Monophosphate-Dependent7078v, cont.Protein Kinase:S.I. Rayter, Expression and Purification of Active abl Protein-Tyrosine Kinase in Escherichia coli.M.J. Zoller, K.E. Johnson, W. Yonemoto, and L. Levin, Functional Expression of Mammalian Adenosine Cyclic Monophosphate-Dependent Protein Kinase in Saccharomyces cerevisiae.M.D. Waterfield and C. Greenfield, Expression and Properties of Epidermal Growth Factor Receptor Expressed from Baculovirus Vectors.D.O. Morgan, J.M. Kaplan, J.M. Bishop, and H.E. Varmus, Production of p60c-src by Baculovirus Expression and Immunoaffinity Purification.L. Ellis and B.A. Levine, Use of Recombinant Baculoviruses and 1H NMR to Study Tyrosine Phosphorylation by Insulin Receptor Protein-Tyrosine Kinase.S. Stabel, D. Schaap, and P.J. Parker, Expression of Protein Kinase C Isotypes Using Baculovirus Vectors.J.C. Stone, M. Moran, and T. Pawson, Construction and Expression of Linker Insertion and Site-Directed Mutants of v-fps Protein-Tyrosine Kinase.Index.