Protein Liquid Chromatography


  • M. Kastner

This book is about liquid chromatography as a tool for isolating and purifying proteins and appears as a handbook-wise linear selection of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase. Part B deals with various target protein classes like membrane proteins, recombinant proteins, and glycoproteins. Finally, Part C looks at various miscellaneous, mutual aspects including coupling reactions, buffer solution additives, and software.
The book as a whole can be viewed as a systematic survey of what can be utilized and optimized these days but it also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the essence of the whole contribution.
It has been considered very important in this compilation to adhere to the philosophy that practical application is everything and thus special attention has been paid to presentation of both classical and recent application examples taken from the literature involving conventional, middle and high pressure techniques. Over-exposure to history, concept and theory has purposely been avoided.
Here, the reader will find a wealth of tips and tricks from users for users, including fair advice as to the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area. A true laboratory companion for work at the bench, right here and now.
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For graduate students, technicians, laboratories and libraries in university departments (especially biochemistry, biology, medicine, pharmacology, toxicology, physiology, veterinary medicine, biotechnology, and bioengineering) as well as the biotechnological and pharmaceutical industries.


Book information

  • Published: December 1999
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-50211-7


This is a must book for every biology and biochemistry scientist, bioanalytical and analytical chemist interested in protein separations. This volume is highly recommended.
Haleem J. Issaq, Ph.D., Editor, The Book Corner, Journal of Liquid Chromatography, Vol. 24, No. 3, 2001

Table of Contents

Part and chapter headings:Individual Chromatographic Modes. Ion exchange chromatography (P.H. Roos). Size exclusion chromatography (M. Rögner). Reversed-phase chromatography (H. Schlüter). Hydrophobic interaction chromatography (L.R. Jacob). Hydroxyapatite chromatography (R. Lukačin, W.R. Deppert). Immobilized metal ion affinity chromatography (M. Kastner). Chromatofocusing (R. Lukačin, W.R. Deppert). Dye-ligand affinity chromatography (J. Kirchberger, H.-J. Böhme). Immobilized artificial membrane chromatography (K. Hauer et al.). Liquid-liquid partition chromatography (U.-B. Hansson, C. Wingren). Displacement chromatography (H. Schlüter, J. Jankowski). Various Target Protein Classes. Integral membrane proteins (G. Welling, S. Welling-Wester). Recombinant proteins (O. Huber, M. Huber-Wunderlich). DNA-binding proteins (M. Velleman). Lectins and glycoconjugates (H.-J. Gabius). Covalent chromatography (D. Whitney). Cell affinity chromatography (D.D. Putnam et al.). Miscellaneous Chromatographic Aspects. Coupling reactions (E. Müller). Immobilization of nucleotides (E. Müller, M. Morr). Miscellaneous biospecific affinity gels (A.M. Williams). Scale-up of downstream processing (J. Walter). Chromatography on porous matrices (J. Kruip). Chromatography using Strep-tag affinity peptides (H. N. Müller, T.G.M. Schmidt). Buffers and additives (W.R. Deppert, R. Lukačin). Chromatography software (T. Steger-Hartmann). Supplement. Abbreviations used in this book. Directory of manufacturers and suppliers. Subject index.