Protein Engineering for Therapeutics, Part A book cover

Protein Engineering for Therapeutics, Part A

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 392 Pages

Published: January 2012

Imprint: Academic Press

ISBN: 978-0-12-416039-2


    1. Simplified Synthetic Antibody Libraries
      Saravanan Rajan and Sachdev S. Sidhu

    2. Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting
      Jijie Gu and Tariq Ghayur

    3. Discovery of internalizing antibodies to tumor antigens from phage libraries
      Yu Zhou and James D Marks

    4. Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments
      Brian D. Harms, Jeffrey D. Kearns, Stephen V. Su, Neeraj Kohli, Ulrik B. Nielsen and Birgit Schoeberl

    5. Cell-specific siRNA delivery by peptides and antibodies
      Sang-Kyung Lee, Alyssa Siefert, Jagadish Beloor, Tarek M Fahmy and Priti Kumar

    6. Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues
      Robert P. Lyon*, David L. Meyer, Jocelyn R. Setter, and Peter D. Senter

    7. Building and characterizing antibody-targeted lipidic nanotherapeutics
      Dmitri B. Kirpotin, Charles O. Noble, Mark E. Hayes, Zhaohua Huang, Tad Kornaga, Yu Zhou, Ulrik B. Nielsen, James D. Marks, and Daryl C. Drummond

    8. Cell-Targeting Fusion Constructs Containing Recombinant Gelonin
      Mi-Ae Lyu; Yu (Joshua) Cao; Khalid A. Mohamedali; and Michael G. Rosenblum

    9. Stapled Peptides for Intracellular Drug Targets
      Gregory L. Verdine andGerard J. Hilinski

    10. Mapping of vascular zip codes by phage display
      Tambet Teesalu, Kazuki N. Sugahara & Erkki Ruoslahti

    11. Engineering cyclic peptide toxins
      Richard J. Clark and David J. Craik

    12. Peptide Discovery using Bacterial Display and Flow Cytometry
      Jennifer A. Getz, Tobias D. Schoep, Patrick S. Daugherty

    13. Oral enzyme therapy for celiac sprue
      Michael T Bethune1 and Chaitan Khosla

    14. Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins
      Jo E. Lomax, ChelcieH. Eller, and Ronald T. Raines

    15. Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer
      Jason R. Cantor, Vasiliki Panayiotou, Giulia Agnello, George Georgiou and Everett M. Stone

    16. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy
      Rastislav Tamaskovic, Manuel Simon, Nikolas Stefan, Martin Schwill and Andreas Plückthun

    17. Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3)
      Shohei Koide, Akiko Koide, and Daša Lipovšek

    18. Anticalins: small engineered binding proteins based on the lipocalin scaffold
      Michaela Gebauer

    19. T Cell Receptor Engineering
      Jennifer D. Stone, Adam S. Chervin, David H. Aggen, David M. Kranz

    20. Engineering Knottins as Novel Binding Agents
      Sarah J. Moore and Jennifer R. Cochran

    21. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier
      William M. Pardridge and Ruben J. Boado

    22. Practical Theoretic Guidance for the Design of Tumor Targeting Agents
      K. Dane Wittrup, Greg M. Thurber, Michael M. Schmidt, John J. Rhoden

    23. Fusions of elastin-like polypeptides to pharmaceutical proteins
      Wafa Hassouneh, Sarah R Mac Ewan, Ashutosh Chilkoti

    24. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells
      David B. Thompson, James J. Cronican, and David R. Liu


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