Protein Engineering for Therapeutics, Part A

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds
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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: January 2012
  • ISBN: 978-0-12-416039-2

Table of Contents

  1. Simplified Synthetic Antibody Libraries
  2. Saravanan Rajan and Sachdev S. Sidhu

  3. Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting
  4. Jijie Gu and Tariq Ghayur

  5. Discovery of internalizing antibodies to tumor antigens from phage libraries
  6. Yu Zhou and James D Marks

  7. Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments
  8. Brian D. Harms, Jeffrey D. Kearns, Stephen V. Su, Neeraj Kohli, Ulrik B. Nielsen and Birgit Schoeberl

  9. Cell-specific siRNA delivery by peptides and antibodies
  10. Sang-Kyung Lee, Alyssa Siefert, Jagadish Beloor, Tarek M Fahmy and Priti Kumar

  11. Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues
  12. Robert P. Lyon*, David L. Meyer, Jocelyn R. Setter, and Peter D. Senter

  13. Building and characterizing antibody-targeted lipidic nanotherapeutics
  14. Dmitri B. Kirpotin, Charles O. Noble, Mark E. Hayes, Zhaohua Huang, Tad Kornaga, Yu Zhou, Ulrik B. Nielsen, James D. Marks, and Daryl C. Drummond

  15. Cell-Targeting Fusion Constructs Containing Recombinant Gelonin
  16. Mi-Ae Lyu; Yu (Joshua) Cao; Khalid A. Mohamedali; and Michael G. Rosenblum

  17. Stapled Peptides for Intracellular Drug Targets
  18. Gregory L. Verdine andGerard J. Hilinski

  19. Mapping of vascular zip codes by phage display
  20. Tambet Teesalu, Kazuki N. Sugahara & Erkki Ruoslahti

  21. Engineering cyclic peptide toxins
  22. Richard J. Clark and David J. Craik

  23. Peptide Discovery using Bacterial Display and Flow Cytometry
  24. Jennifer A. Getz, Tobias D. Schoep, Patrick S. Daugherty

  25. Oral enzyme therapy for celiac sprue
  26. Michael T Bethune1 and Chaitan Khosla

  27. Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins
  28. Jo E. Lomax, ChelcieH. Eller, and Ronald T. Raines

  29. Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer
  30. Jason R. Cantor, Vasiliki Panayiotou, Giulia Agnello, George Georgiou and Everett M. Stone

  31. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy
  32. Rastislav Tamaskovic, Manuel Simon, Nikolas Stefan, Martin Schwill and Andreas Plückthun

  33. Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3)
  34. Shohei Koide, Akiko Koide, and Daša Lipovšek

  35. Anticalins: small engineered binding proteins based on the lipocalin scaffold
  36. Michaela Gebauer

  37. T Cell Receptor Engineering
  38. Jennifer D. Stone, Adam S. Chervin, David H. Aggen, David M. Kranz

  39. Engineering Knottins as Novel Binding Agents
  40. Sarah J. Moore and Jennifer R. Cochran

  41. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier
  42. William M. Pardridge and Ruben J. Boado

  43. Practical Theoretic Guidance for the Design of Tumor Targeting Agents
  44. K. Dane Wittrup, Greg M. Thurber, Michael M. Schmidt, John J. Rhoden

  45. Fusions of elastin-like polypeptides to pharmaceutical proteins
  46. Wafa Hassouneh, Sarah R Mac Ewan, Ashutosh Chilkoti

  47. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells

    David B. Thompson, James J. Cronican, and David R. Liu