Plant Tissue Culture

Techniques and Experiments


  • Roberta Smith, Emeritus Professor, Department of Horticulture, Vegetable Crops Improvement Center, Texas A&M University, College Station, Texas, U.S.A.

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.
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Faculty, graduate students, and undergraduates interested in plant cell and tissue culture.


Book information

  • Published: July 2012
  • ISBN: 978-0-12-415920-4


strength of this book lies in its descriptions of essential concepts, introduction to a wide range of major applications, and simple protocols to provide empirical demonstrations. ...this book would be a good teaching resource reference for instructors wishing to design their own laboratory demonstations."
--Phil Bregitzer, Germplasm Research Facility, in CROP SCIENCE SOCIETY OF AMERICA (June 2001)

"...presents a structured programme using plant material that is available all year round. ...I was impressed with the gradual introduction of complex techniques and ideas. ...the book is well written...a useful contribution to teaching in plant tissue culture."
-Ronald A. de Fossard in TRENDS IN PLANT SCIENCE (February 2001)
"This is an excellent laboratory manual. The student can learn many plant tissue culture techniques commonly used today."
Praise for the First Edition, --HORTSCIENCE (1995)
"This book is an excellent overview of important tissue culture techniques. It is highly recommended for laboratory courses in tissue culture and would be useful in research laboratories where tissue culture techniques are required. Practical aspects of tissue culture are explained in a clear and concise way. This is essential for the novice and useful for experienced individuals planning a new course or laboratory. The manual is commended for providing information required for successful tissue culture at the fingertips of students."

Table of Contents

Plant Tissue Culture: Techniques and ExperimentsThird EditionRoberta H. Smith

Table of ContentsPrefaceAcknowledgmentsScheduling and Interrelationships of Exercises1. History of Plant Cell CultureTrevor A. Thorpe2. Setup of a Tissue Culture Laboratory3. Media Components and Preparation4. Explant Preparation5. Contamination6. Callus Induction7. Regeneration and Morphogenesis8. Woody Shrubs and TreesBrent H. McCown9. Haploid Plants from Anther Culture10. Embryo Rescue11. Meristem Culture for Virus-Free Plants12. In Vitro Propagation for Commercial Production of Ornamentals13. Protoplast Isolation and FusionJungeun Kim Park, Sunghun Park, & James E. Craven14. Agrobacterium-Mediated Transformation of PlantsJungeun Kim Park, Sunghun Park, Qingyu Wu, & Stuart SpragueAppendix I: Useful MeasurementsAppendix II: Review of Solution PreparationAppendix III: List of SuppliersAppendix IV: Common Plant Tissue Culture Terms