Plant Tissue Culture book cover

Plant Tissue Culture

Techniques and Experiments

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.

The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.

Paperback, 208 Pages

Published: July 2012

Imprint: Academic Press

ISBN: 978-0-12-415920-4


  • strength of this book lies in its descriptions of essential concepts, introduction to a wide range of major applications, and simple protocols to provide empirical demonstrations. ...this book would be a good teaching resource reference for instructors wishing to design their own laboratory demonstations."
    --Phil Bregitzer, Germplasm Research Facility, in CROP SCIENCE SOCIETY OF AMERICA (June 2001)

    "...presents a structured programme using plant material that is available all year round. ...I was impressed with the gradual introduction of complex techniques and ideas. ...the book is well written...a useful contribution to teaching in plant tissue culture."
    -Ronald A. de Fossard in TRENDS IN PLANT SCIENCE (February 2001)
    "This is an excellent laboratory manual. The student can learn many plant tissue culture techniques commonly used today."
    Praise for the First Edition, --HORTSCIENCE (1995)
    "This book is an excellent overview of important tissue culture techniques. It is highly recommended for laboratory courses in tissue culture and would be useful in research laboratories where tissue culture techniques are required. Practical aspects of tissue culture are explained in a clear and concise way. This is essential for the novice and useful for experienced individuals planning a new course or laboratory. The manual is commended for providing information required for successful tissue culture at the fingertips of students."


  • Plant Tissue Culture: Techniques and Experiments

    Third Edition

    Roberta H. Smith

    Table of Contents



    Scheduling and Interrelationships of Exercises

    1. History of Plant Cell Culture

    Trevor A. Thorpe

    2. Setup of a Tissue Culture Laboratory

    3. Media Components and Preparation

    4. Explant Preparation

    5. Contamination

    6. Callus Induction

    7. Regeneration and Morphogenesis

    8. Woody Shrubs and Trees

    Brent H. McCown

    9. Haploid Plants from Anther Culture

    10. Embryo Rescue

    11. Meristem Culture for Virus-Free Plants

    12. In Vitro Propagation for Commercial Production of Ornamentals

    13. Protoplast Isolation and Fusion

    Jungeun Kim Park, Sunghun Park, & James E. Craven

    14. Agrobacterium-Mediated Transformation of Plants

    Jungeun Kim Park, Sunghun Park, Qingyu Wu, & Stuart Sprague

    Appendix I: Useful Measurements

    Appendix II: Review of Solution Preparation

    Appendix III: List of Suppliers

    Appendix IV: Common Plant Tissue Culture Terms


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