Oxygen Sensing

Edited by

  • Gregg Semenza, John Hopkins University School of Medicine, Baltimore, Maryland
  • Chandan Sen, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA

The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.
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Biochemists, cell biologists, molecular biologists, and geneticists


Book information

  • Published: March 2004
  • ISBN: 978-0-12-182785-4

Table of Contents

LUNG AND THE AIRWAYS1. Assessment of oxygen sensing by model airway and arterial chemoreceptorsP. J. Kemp, C.Peers2. Methods to study neuroepithelial bodies as airway oxygen sensors.E. Cutz, X.W. Fu, H. Yeger3. Role of glutathione redox state in oxygen sensing by carotid body chemoreceptor cells.C. Gonzalez, G. Sanz-Alfayate, A. Obeso, M. T. Agapito4. Determination of signaling pathways responsible for hypoxic pulmonary vasoconstriction: use of the small vessel myographJ. P.T. Ward, V. A. Snetkov5. Oxygen-dependent regulation of pulmonary circulationR.M. Tuder, S. McGrath, N. F. Voelkel, M. W. Geraci6. Detection of oxygen sensing during intermittent hypoxiaN. R. Prabhakar,Y.J. Peng, J. L. Overholt, G K. Kumar7. Functional analysis of the role of HIF-1 in the pathogenesis of hypoxic pulmonary hypertension G. L. Semenza, L. A. ShimodaCARDIOVASCULAR AND BLOOD8. Perceived hyperoxia: oxygen regulated signal transduction pathways in the heartS. Roy, S. Khanna, C. K. Sen9. Evaluation of Cytochrome P450-4A omega-Hydroxylase and 20-HETE as an O2 Sensing Mechnism in the Microcirculation10. Assessment of roles for oxidant mechanisms in vascular oxygen sensingM. S. Wolin, P. M. Kaminski, M. Ahmad, S. A. GupteDepartment of Physiology, New York Medical College, Valhalla, USA.11. Survival surgery for coronary occlusion and reoxygenation in a rodent modelW. A. Wallace, A. J. Cardounel, S. Khanna, C. K. Sen 12. Measurement of in vivo oxidative stress regulated by the Rac1 GTPaseK. Irani, M. Ozaki13. Proteomic analysis of the mammalian cell nucleusA. K. Tewari, S. Roy, S. Khanna, Chandan K. Sen14. Oxygen-dependent regulation of erythropoiesisJ. T. Prchal, J. Jelinek15. Physiological responses to chronic anemia in fetal sheepL. Davis, R. Hohimer16. Hypoxia-Inducible Factor 1 alpha-Deficient Chimeric Mice as a Model to Study Abnormal B Lymphocyte Development and AutoimmunityION CHANNELS17. Molecular strategies for studying oxygen-sensitive K+ channelsE. Honore, K. Buckler18. Oxygen sensing by human recombinant K(+) channel: assessment of the use of stable cell linesP. J. Kemp, D. Iles, C. Peers19. Methods to study oxygen-sensing sodium channelsA. K. M. Hammarstrom, P. W. Gage20. Analysis of oxygen-sensitive human cardiac L-type Ca(2+) channel alpha(1C) subunit (hHT isoform)C. Peers, J. L. Scragg, I. M. Fearon, S. G. Ball, A. Schwartz, G. VaradiTUMOR BIOLOGY21. Analysis of von Hippel-Lindau tumour suppressor as mediator of cellular oxygen sensing P. J. Ratcliffe, N. Masson22. Analysis of the von Hippel-Lindau hereditary cancer syndrome: implications of oxygen sensingW. G. Kaelin Jr., H. Yang, M. Ivan, J-H. Min, W. Kim23. Tumor hypoxia and malignant progressionP. Vaupel, A. Mayer, M. HockelMETABOLISM24. Oxygen dependent regulation of hepatic glucose metabolismT. Kietzmann25. Assessing oxygen sensitivity of the multiple drug resistance (MDR1) geneS. P. Colgan, K. M. Comerford26. Oxygen-dependent regulation of adipogenesisA. J. Giaccia, L. M. Swiersz, Z. YunNERVOUS SYSTEM27. Methods to detect hypoxia-induced ischemic tolerance in the brainF. R. Sharp, M. Bernaudin28. Proximal middle cerebral artery occlusion surgery for the study of ischemia-reoxygenation injury in the brainA.J. Cardounel, W. A. Wallace, C. K. Sen29. Carotid chemodenervation approach to study oxygen sensing in brainstem O. Pascual, J-C. Roux, C. Soulage, M-P. Morin-Surun, M. Denavit-Saubie, J-M. Pequignot30. Discovery of oxygen responsive genes in pheochromocytoma cellsD. E. Millhorn, K. A. Seta, T. K. Ferguson GENERAL31. Oxygen-dependent asparagines hydroxylationJ. Gorman, D. J. Peet, D. Lando, D. A. Whelan, M. L. Whitelaw32. Deciphering of the oxygen sensing pathway by microscopy H. Acker, C. Huckstorf, H. Sauer, T. Streller, M. Wartenberg33. Identification of CREB-dependent transcriptional responses in hypoxia by microarray analysisC. T. Taylor, M. O. Leonard, S. O’Reilly, K. M. ComerfordWOUND HEALING34. Determination of the role of HIF-1 in wound healingJ. E. Albina, J. S. Reichner35. Measuring oxygen in woundsH. W. Hopf, T. K. Hunt, H. Scheuenstuh., J. M. West, L. M. Humphrey, M.D. Rollins36. Regulatory role of lactate in wound repairM. Z. Hussain, Q. P. Ghani, S. Wagner, H. D. Becker, T. K. Hunt37. Protocols for Topical and Systemic Oxygen Treatments in Wound HealingG. M. Gordillo, R. Schlanger, W. A. Wallace, V. Bergdall, R. BartlettUNICELLULAR SYSTEMS38. Measurement of oxidative stress in cells exposed to hypoxia or other changes in oxygen concentrationR. O. Poyton, R. Dirmeier, K. O’Brien, M. Engle, A. Dodd, E. Spears39. Evaluation of oxygen response involving differential gene expression in Chlamydomonas reinhardtiiJ. del Campo, J. M. Quinn, S. Merchant40. Resonance Raman and ligand binding analysis of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis S. Aono, H. Nakajima, T. Ohta, T. Kitagawa41. Analysis of FNR as oxygen sensor in Escherichia coliR. A. Schmitz, S. Achebach, G. Unden42. Experimental strategies for analyzing oxygen sensing in yeastR.O. Poyton, R. Dirmeier, K. O’Brien, P. David, A. DoddPHYSICAL DETECTION OF OXYGEN43. Measuring tissue pO2 with microelectrodes D. G. Buerk44. Identifying oxygen sensors by their photochemical action spectraD. F. Wilson45. Fabrication and in vivo evaluation of NO-releasing electrochemical oxygen sensing cathetersM. E. Meyerhoff, M. C. Frost46. Methods in optical oxygen sensing: protocols and critical analysesD. B. Papkovsky47. Concurrent 31P NMR spectroscopy and fiber-optic oxygen consumption measurements in perfused rat heartsP. Pantano, P. Zhao, Y. Zhao, A. D. Sherry48. MICROXYMETRY: An EPR Spectroscopy Based Method for Simultaneous Measurements of Oxygen Utilization and Free Radicals Production in Enzymatic ReactionsG. Ilangovan, J. L. Zweier, P. Kuppusamy