Tools for Controlling and Monitoring Neuronal Activity

Optogenetics is a fast-growing field concerning the invention, and use, of molecules that are genetically expressed in cells, and then either report on cellular physiology in optical form, or enable control of specific pathways in cells when actuated by light. This book reviews the current state, as well as the underlying principles and future directions, of a diversity of optogenetic tools of importance for observing and controlling cellular signaling and physiology.

These tools are already revolutionizing neuroscience, and are starting to have impact on fields ranging from metabolism to development to cardiology. The book contains a dozen chapters written by world experts on these topics, half focusing on the optogenetic molecular tools themselves, and half on the genetic and hardware approaches that make them powerfully useful in intact tissues and organisms.

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Neuroscientists, psychologists, neurologists


Book information

  • Published: March 2012
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-59426-6

Table of Contents

  1. A comprehensive concept of optogenetics
  2. Guillaume P. Dugué, Walther Akemann, and Thomas Knöpfel

  3. Optogenetic excitation of neurons with channelrhodopsins: light instrumentation, expression systems, and channelrhodopsin variants
  4. John Y. Lin

  5. Genetically encoded molecular tools for light-driven silencing of targeted neurons
  6. Brian Y. Chow, Xue Han, and Edward S. Boyden

  7. Genetically encoded probes for optical imaging of brain electrical activity
  8. Amelie Perron, Walther Akemann, Hiroki Mutoh and Thomas Knöpfel

  9. Neural activity imaging with genetically encoded calcium indicators
  10. Lin Tian, Jasper Akerboom, Eric R. Schreiter, and Loren L. Looger

  11. Manipulating cellular processes using optical control of protein-protein interactions
  12. Chandra L. Tucker

  13. Two-photon optogenetics
  14. Dan Oron, Eirini Papagiakoumou, F. Anselmi, and Valentina Emiliani

  15. Zebrafish as an appealing model for optogenetic studies
  16. Joshua Simmich, Eric Staykov, and Ethan Scott

  17. Genetic targeting of specific neuronal cell types in the cerebral cortex
  18. Alan Urban, and Jean Rossier

  19. Mouse transgenic approaches in optogenetics
  20. Hongkui Zeng, and Linda Madisen

  21. Optogenetics in the nonhuman primate
  22. Xue Han

  23. Optogenetic Reporters: Fluorescent Protein-Based Genetically-Encoded Indicators of Signaling and Metabolism in the Brain

    Mathew Tantama, Yin Pun Hung,and Gary Yellen