Nuclear Magnetic Resonance of Biological Macromolecules, Part C

Methods in Enzymology

Edited by

  • Thomas James, School of Pharmacy, University of California, San Francisco, U.S.A.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules.
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Biochemists, cell biologists, molecular biologists, geneticists


Book information

  • Published: April 2005
  • ISBN: 978-0-12-182799-1

Table of Contents

Identification and optimization of protein domains for NMR studiesIn-cell NMR spectroscopyMolecular Fragment Replacement Approach to Protein Structure Determination by Chemical Shift and Dipolar Homology Database MiningCross-correlated relaxation for structure and dynamicsRapid NMR Data CollectionAn Integrated Platform for Automated Analysis of Protein NMR StructuresRapid Assessment of Protein Structural Stability and Fold Validation via NMR Determination of Protein Backbone Structures from Residual Dipolar CouplingsRobotic Cloning and Protein Production Platform of the Northeast Structural Genomics ConsortiumProtein Structure Estimation From Minimal Restraints Using RosettaProtein Structure Elucidation From Minimal Data: The CLOUDS and ABACUS ApproachesElucidation of the Protein Folding Landscape by NMRMembrane Protein Preparation for TROSY NMR ScreeningSolution structure and dynamics of integral membrane proteins by NMR: a case study involving the enzyme PagPNMR Experiments on Aligned Samplet of Membrane ProteinsNMR techniques used with very large biological macromolecules in solutionStructure determination of large biological RNAsHydrodynamic Models and Computational Methods for NMR relaxationSolution NMR Spin Relaxation Methods for Characterizing Chemical Exchange in High Molecular Weight SystemsIsotropic Reorientational Eigenmode Dynamics Complements NMR Relaxation Measurements for RNANMR techniques for identifying the interface of a larger protein-protein complex: cross and transferred cross-saturation experimentsEnzyme Dynamics During Catalysis Measured by NMR SpectroscopyStructure determination of protein/RNA complexes by NMRUtilization of NMR-derived fragment leads in drug designDiscovery of Ligands by a Combination of Computational and NMR-based Screening: RNA as an Example Target