Neurons: Methods and Applications for the Cell BiologistEdited by
- Peter Hollenbeck, Purdue University, West Lafayette, U.S.A.
- James Bamburg, Colorado State University, Fort Collins, U.S.A.
Written by cell biologists and neuroscientists, this volume in the Methods in Cell Biology series is a detailed guide for cell biologists who would like to approach their experimental questions using nature's most interesting cells - neurons. Many cell biologists study the properties of cells grown outside of the animal in the controlled environment of a culture dish, but they would benefit from directing their attention to nerve cells as they are well-suited to almost every area of cell biological inquiry. This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes into neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.With the current and reliable information contained in this volume, growing various kinds of neurons in culture and using them for diverse experimental applications will be easy for cell biologists to master!
Cell and Molecular Biologists, Developmental Biologists, and Neuroscientists
Methods in Cell Biology
Paperback, 500 Pages
Published: July 2003
Imprint: Academic Press
". . .invaluable for workers at all levels of cell biology." -NATURE
- Comparing the Properties of Neuronal Culture Systems Growing and Working with Peripheral NeuronsDissection and Culturing of Chick Ciliary Ganglion NeuronsThe Culture of Chick Forebrain NeuronsGrowing and Working with Spinal Motor NeuronsAvian Purkinje Neuronal CulturesCulturing Hippocampal and Cortical NeuronsWorking with Xenopus Spinal Neurons in Live Cell CulturesCulturing Neurons from the Snail HelisomaThe Ti1 Pioneer PathwayTechniques to Dissect Cellular and Subcellular Function in Drosphila Nervous SystemPC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.