Natural Product Biosynthesis by Microorganisms and Plants, Part A

Edited by

  • David Hopwood, John Innes Centre and AFRC Institute of Plant Science Research, Norwich, UK

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, Terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.
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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: October 2012
  • ISBN: 978-0-12-394290-6

Table of Contents

  1. Steady-state kinetic characterization of sesquiterpene synthases by gas chromatography-mass spectroscopy
    Steven R. Garrett, Richard J. Morris, and Paul E. O’Maille
  2. Automating gene library synthesis by structure-based combinatorial protein engineering (SCOPE): examples from plant sesquiterpene synthases
    Melissa Dokarry, Caroline Laurendon, Paul E. O’Maille
  3. In planta Transient Expression Analysis of Monoterpene Synthases
    Sol A. Green, Xiuyin Chen and Adam J. Matich
  4. Natural Rubber Biosynthesis in Plants: Rubber Transferase
    Katrina Cornish and Wenshuang Xie
  5. Discovery and Characterization of Terpenoid Biosynthetic Pathways of Fungi
    Grayson T. Wawrzyn, Sarah E. Bloch and Claudia Schmidt-Dannert
  6. Menaquinone biosyntheses in microorganisms
    Tohru Dairi
  7. Diversity and analysis of bacterial terpene synthases
    Yuuki Yamada, David E. Cane and Haruo Ikeda
  8. Platensimycin and platencin biosynthesis in Streptomyces platensis, showcasing discovery and characterization of novel bacterial diterpene synthases
    Michael J. Smanski, Ryan M. Peterson, and Ben Shen
  9. Strategies for Engineering Plant Natural Products:The Iridoid-Derived Monoterpene Indole Alkaloids of Catharanthus roseus
    Sarah O'Connor
  10. Discovery and functional analysis of monoterpenoid indole alkaloid pathways in plants
    Vincenzo De Luca, Vonny Salim, Dylan Levac1, Sayaka Masada Atsumi and Fang Yu
  11. Biochemical Genomics for Gene Discovery in Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy and Related Species
    Thu Thuy T. Dang, Akpevwe Onoyovwi, Scott C. Farrow and Peter J. Facchini
  12. Analysis and modification of ergot alkaloid profiles in fungi
    Daniel G. Panaccione, Katy L. Ryan, Christopher L. Schardl, and Simona Florea
  13. Engineering of glucosinolate biosynthesis: candidate gene identification and validation
    Morten E Møldrup, Bo Salomonsen, Barbara A Halkier
  14. Structure, function and evolutionary analyses of the plant type III polyketide synthase family
    Jing-Ke Weng and Joseph P. Noel
  15. Engineering of plant type III polyketide synthases
    Toshiyuki Wakimoto, Hiroyuki Morita, and Ikuro Abe
  16. Type III polyketide synthases in microorganisms
    Yohei Katsuyama and Yasuo Ohnishi