Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas
By- A.M. Campbell
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
Included in series
Laboratory Techniques in Biochemistry and Molecular Biology
Laboratory Techniques in Biochemistry and Molecular Biology
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Published: January 1984
Imprint: Elsevier
ISBN: 978-0-444-80592-8
Reviews
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Clearly written and founded on solid experience and scholarship, this concise book is one of the best sources of practical information on the subject published thus far
JOURNAL OF IMMUNOLOGICAL METHODS
Contents
- General properties and applications of monoclonal antibodies. Assay techniques. Selection of animals and cell lines. Immunisation. Cell culture requirements for hybridomas. Fusion procedures. Transformation. Selection and cloning. Antibody production and purification. Characterisation of monoclonal antibodies.
