Molecular Protocols in Transfusion Medicine
- Gregory Denomme, Mount Sinai Hospital and The Canadian Blood Services, Toronto, Canada
- Maria Rios, New York Blood Center, New York, U.S.A.
- Marion Reid, New York Blood Center, New York, U.S.A.
This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines.
Hematologists, blood bank technologists and students, and immunologists.