Molecular Microbiology Laboratory

A Writing-Intensive Course

By

  • Walt Ream, Professor, Department of Microbiology, Oregon State University
  • Bruce Geller, Professor, Department of Microbiology, Oregon State University
  • Janine Trempy, Professor, College of Science, Department of Microbiology, Oregon State University
  • Katherine Field, Associate Professor, Department of Microbiology, Oregon State University

Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.
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Audience

Appropriate for college sophomores through seniors in any of the life sciences, including microbiology, genetics, general biology, biochemistry, molecular biology, botany, zoology, etc. The experiments are also appropriate for properly equipped junior colleges and liberal arts institutions.

 

Book information

  • Published: August 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-397044-2

Reviews

"This book is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports."--Anticancer Research, August 2013




Table of Contents

Introduction
Experiment 1: Plasmid Purification and Restriction Mapping
Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein
Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities
Appendix A: Sample Laboratory Reports
Appendix B: Sample Proposals
Appendix C: Sample Tests
Appendix D: Guidance for Presentations
Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991)
Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997)
Appendix G: Sequence Editing Tutorial
Appendix H: Peak Scanner Tutorial
Appendix I: Adding Size Standards to Peak Scanner
Appendix J: PRIMER Tutorial
Appendix K: Checklist for Grading Laboratory Reports
Appendix L: Peer Review Checklist
Appendix M: Career Advice
Appendix N: Molecular Microbiology Laboratory Preparation Manual
Bibliography
Index