- Liza Pon
- Eric Schon, Columbia University, Neurology Department, New York, U.S.A.
This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.View full description
Researchers and graduate students in cell biology, developmental biology, and genetics. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.
- Published: April 2007
- Imprint: ACADEMIC PRESS
- ISBN: 978-0-12-544173-5
Table of ContentsIsolation and Subfractionation of Mitochondria1. Isolation and Subfractionation of Mitochondria in Animal Cells2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae3. Isolation and Subfractionation of Mitochondria from PlantsBiochemical Assays of Mitochondrial Activity4. Biochemical Assays of Respiratory Chain Complex Activity5. Polarographic Assays of Respiratory Chain Complex Activity6. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria7. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues8. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures9. Assays of Fatty Acid ƒÒ-Oxidation Activity10. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc11. Assays of Cardiolipin Levels12. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems13. Methods for Studying Fe/S Cluster Protein Maturation in Yeast MitochondriaAssays for Mitochondrial Respiratory Activity and Permeability in Living Cells14. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores15. Biosensors for the Detection of Calcium and pH16. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria17. Luciferase Expression for ATP Imaging: Application to Cardiac MyocytesOxidative Stress Measurements18. Measurement of Reactive Oxygen Species in Cells and Mitochondria19. Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase20. Methods for Measuring the Regulation of Respiration by Nitric Oxide21. Methods for Determining the Modification of Protein Thiols by Reactive LipidsMitochondrial Genes and Gene Expression22. Detection of Mutations in mtDNA23. Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans24. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions25. Transmitochondrial Technology in Animal Cells26. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas Reinhardtii Mitochondria27. Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases28. In vivo and In organello Analysis of Mitochondrial TranslationAssays for Mitochondrial Morphology and Motility29. Visualization of Mitochondria in Budding Yeast30. Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells31. Cell-Free Assays for Mitochodnrial-Cytoskeletal Interactions32. Assays to Measure Mitochondrial Fusion In Vitro and the GTPase Activity of Mitochondrial Fission ProteinsMethods to Determine Protein Localization to Mitochondria33. Electrophoretic Methods to Isolate Protein Complexes from Mitochondria34. Analysis of Protein-Protein Interactions in Mitochondria35. Analysis and Prediction of Mitochondrial Targeting Signals36. Import of Proteins into MitochondriaAppendicesAppendix 1: Basic Properties of MitochondriaAppendix 2: Direct and Indirect Inhibitors of Mitochondrial ATP SynthesisAppendix 3: Linearized Maps of Mitochondrial Genomes from Representative OrganismsAppendix 4: Mitochondrial Genetic Codes in Various OrganismsAppendix 5: Gene Products Present in Mitochondria of Yeast and Animal CellsAppendix 6: Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions