Microtubules: in vivo

Edited by

  • Lynne Cassimeris
  • Phong Tran, University of Pennsylvania, Philadelphia, USA and Institut Curie, Paris, France

This volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.
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cell biologists, biophysicists and pharmacologists as well as for researchers in the biomedical and biotechnology communities.


Book information

  • Published: August 2010
  • ISBN: 978-0-12-381349-7

Table of Contents

  1. Microtubule dynamics at the cell cortex probed by TIRF microscopy
  2. Ilya Grigoriev and Anna Akhmanova

  3. Microscopy methods for the study of centriole biogenesis and function in Drosophila
  4. Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

  5. Quality control in single molecule studies of kinesins and microtubule-associated proteins
  6. Gary J. Brouhard

  7. Imaging cilia in zebrafish
  8. Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

  9. Automatic Identification of Microtubules in Cellular Electron Tomography
  10. Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

  11. Imaging and analysis of the microtubule cytoskeleton in Giardia
  12. Scott C. Dawson and Susan A. House

  13. Kinetochore-microtubule dynamics and attachment stability
  14. Jennifer G. DeLuca

  15. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
  16. Gohta Goshima

  17. Live cell imaging techniques for analyses of microtubules in Dictyostelium
  18. Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf

  19. Microtubule dynamics in dendritic spines
  20. Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

  21. Analysis of Microtubules in Budding Yeast
  22. Alexander Rauch, Elena Nazarova and Jackie Vogel

  23. Determination of Microtubule Dynamic Instability in Living Cells
  24. Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

  25. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos
  26. Akatsuki Kimura1 and Shuichi Onami

  27. Cryo electron tomography of cellular microtubules
  28. Roman I. Koning

  29. Microtubule dynamics in plant cells
  30. Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd

  31. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function
  32. Sara Moutinho-Pereira, Irina Matos and Helder Maiato

  33. Protein micropatterns: a direct printing protocol using deep UVs
  34. Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel

  35. Optical trapping and laser ablation of microtubules in fission yeast
  36. Nicola Maghelli and Iva M. Tolić-Nørrelykke

  37. Melanophores for Microtubule dynamics and motility assays
  38. Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov

  39. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation
  40. Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin

  41. Imaging of mitotic spindle dynamics in C. elegans embryos
  42. Mika Toya, Yumi Iida and Asako Sugimoto

  43. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast
  44. Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran

  45. Microtubule-dependent spatial organization of mitochondria in fission yeast
  46. Maitreyi Das, Stephan Chiron and Fulvia Verde

  47. Photoactivatable GFP-Tubulin
  48. U. Serdar Tulu*, Nick P. Ferenz

  49. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics
  50. Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw

  51. Analysis of microtubule polymerization dynamics in live cells

Sarah Gierke, Praveen Kumar, and Torsten Wittmann