Microtubules: in vivo book cover

Microtubules: in vivo

This volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

Audience
cell biologists, biophysicists and pharmacologists as well as for researchers in the biomedical and biotechnology communities.

Included in series
Methods in Cell Biology

Hardbound, 552 Pages

Published: August 2010

Imprint: Academic Press

ISBN: 978-0-12-381349-7

Contents

    1. Microtubule dynamics at the cell cortex probed by TIRF microscopy
    2. Ilya Grigoriev and Anna Akhmanova

    3. Microscopy methods for the study of centriole biogenesis and function in Drosophila
    4. Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

    5. Quality control in single molecule studies of kinesins and microtubule-associated proteins
    6. Gary J. Brouhard

    7. Imaging cilia in zebrafish
    8. Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

    9. Automatic Identification of Microtubules in Cellular Electron Tomography
    10. Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

    11. Imaging and analysis of the microtubule cytoskeleton in Giardia
    12. Scott C. Dawson and Susan A. House

    13. Kinetochore-microtubule dynamics and attachment stability
    14. Jennifer G. DeLuca

    15. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
    16. Gohta Goshima

    17. Live cell imaging techniques for analyses of microtubules in Dictyostelium
    18. Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf

    19. Microtubule dynamics in dendritic spines
    20. Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

    21. Analysis of Microtubules in Budding Yeast
    22. Alexander Rauch, Elena Nazarova and Jackie Vogel

    23. Determination of Microtubule Dynamic Instability in Living Cells
    24. Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

    25. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos
    26. Akatsuki Kimura1 and Shuichi Onami

    27. Cryo electron tomography of cellular microtubules
    28. Roman I. Koning

    29. Microtubule dynamics in plant cells
    30. Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd

    31. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function
    32. Sara Moutinho-Pereira, Irina Matos and Helder Maiato

    33. Protein micropatterns: a direct printing protocol using deep UVs
    34. Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel

    35. Optical trapping and laser ablation of microtubules in fission yeast
    36. Nicola Maghelli and Iva M. Tolić-Nørrelykke

    37. Melanophores for Microtubule dynamics and motility assays
    38. Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov

    39. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation
    40. Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin

    41. Imaging of mitotic spindle dynamics in C. elegans embryos
    42. Mika Toya, Yumi Iida and Asako Sugimoto

    43. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast
    44. Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran

    45. Microtubule-dependent spatial organization of mitochondria in fission yeast
    46. Maitreyi Das, Stephan Chiron and Fulvia Verde

    47. Photoactivatable GFP-Tubulin
    48. U. Serdar Tulu*, Nick P. Ferenz

    49. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics
    50. Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw

    51. Analysis of microtubule polymerization dynamics in live cells

    Sarah Gierke, Praveen Kumar, and Torsten Wittmann

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