Microtubules, in vitro
Edited by- Leslie Wilson, University of California, Santa Barbara, U.S.A.
- John Correia, University of Mississippi Medical Center, Jackson, USA
- John Correia, University of Mississippi Medical Center, Jackson, USA
- Leslie Wilson, University of California, Santa Barbara, U.S.A.
Included in series
Methods in Cell Biology
Methods in Cell Biology
Hardbound,
Published: June 2010
Imprint: Academic Press
ISBN: 978-0-12-374815-7
Contents
- Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes.
- Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography Herbert P. Miller and Leslie Wilson
- Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity Adrian Begaye and Dan L Sackett
- Isolating Tubulin From Nonneural Sources Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette
- Characterization of Anti-b -Tubulin Antibodies Anthony J. Spano* and Anthony Frankfurter
- Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction Sharon Lobert, Laree Hiser and John J. Correia
- Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes Mónica López-Fanarraga, Gerardo Carranza, Raquel Castaño, Sofia Nolasco, J. Avila and J.C. Zabala
- Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins Redeker, Virginie
- Methods in Tubulin Proteomics Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard Section 2: Microtubule Structure and Dynamics
- Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions Eva Nogales, Vincent H. Ramey and Hong-Wei Wang
- High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy Loic Hamon, Patrick A. Curmi, David Pastré
- Holly V. Goodson and Ivan V. Gregoretti Using computational modeling to understand microtubule dynamics: a primer for cell biologists
- Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev Mythili Yenjerla, Manu Lopus and Leslie Wilson
- Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt
- Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schäffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard
- Studying kinesin motors by optical 3D-nanometry in gliding motility assays Bert Nitzsche, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez Section 3: Drugs
- Analysis of tubulin oligomers by Analytical Ultracentrifugation. J.J. Correia
- Determination of Drug Binding to Microtubules in vitro Jennifer A. Smith and Mary Ann Jordan
- Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda
- A Tubulin polymerization micro-assay used to compare ligand efficacy Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.
- Fluorescent Taxoid Probes for Microtubule Research Isabel Barasoain, J. Fernando Díaz, and José M. Andreu
- The binding of vinca domain agents to tubulin: structural and biochemical studies Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoît Gigant Section 4: Interactions with Motors and MAPs
- Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance Marie-Jeanne Clément, Philippe Savarin, Elisabeth Adjadj, André Sobel, Flavio Toma, Patrick A. Curmi
- Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR François Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot
- Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein
- Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM) Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman
- Multiple color single molecule TIRF imaging and tracking of MAPs and motors Jennifer L. Ross and Ram Dixit
- Dixit and Ross - final submitted 11/4 & lost - resubmitted 1/8/10
- Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey Section 5: Functional Extracts and Force Measurements
- Quantitative Characterization of filament dynamics by single-molecule lifetime measurements. Leonid A. Mirny and Daniel J. Needleman
- Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle. Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin
- In vitro assays to study force generation at dynamic microtubule ends Liedewij Laan and Marileen Dogterom
- Reconstitution and functional analysis of kinetochore subcomplexes Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman
- In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces
