MicroRNA Methods

Edited by

John Rossi, City of Hope's Beckman Research Institute, Duarte, California, U.S.A.

Audience
Biochemists, molecular and cell biologists.

Included in series
Methods in Enzymology

Hardbound, 304 Pages

Published: August 2007

Imprint: Academic Press

ISBN: 978-0-12-373917-9

SECTION I. Identifying Micro RNAs and their targets Chapter 1: Identification of viral miRNAsChapter 2: Robust machine learning algorithms predict microRNA genes and targets Chapter 3: Idenfication of virally encoded microRNAs Chapter 4: Computational Methods for microRNA Target Prediction SECTION II. Micro RNA expression, maturation and functional analysisChapter 5: In vitro and in vivo assays for the activity of the Drosha-DGCR8 complexChapter 6: Microarray analysis of microRNA gene expression.Chapter 7: Cloning and detecting signature microRNAs from mammalian cells Chapter 8: Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivoChapter 9: Analysis of small RNA profiles during development.Chapter 10: Dissecting MicroRNA-mediated Gene Regulation and Function in T Cell DevelopmentSECTION III. MiRNAs and DiseaseChapter 11: Investigation of microRNAs alterations in leukemias and lymphomasChapter 12: Discovery of pathogen-regulated small RNAs in plantsChapter 13: Protocols for expression and functional analysis of viral microRNAs

Contents