MicroRNA Methods

Edited by

  • John Rossi, City of Hope's Beckman Research Institute, Duarte, California, U.S.A.

MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.
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Biochemists, molecular and cell biologists.


Book information

  • Published: August 2007
  • ISBN: 978-0-12-373917-9

Table of Contents

SECTION I. Identifying Micro RNAs and their targets Chapter 1: Identification of viral miRNAsChapter 2: Robust machine learning algorithms predict microRNA genes and targets Chapter 3: Idenfication of virally encoded microRNAs Chapter 4: Computational Methods for microRNA Target Prediction SECTION II. Micro RNA expression, maturation and functional analysisChapter 5: In vitro and in vivo assays for the activity of the Drosha-DGCR8 complexChapter 6: Microarray analysis of microRNA gene expression.Chapter 7: Cloning and detecting signature microRNAs from mammalian cells Chapter 8: Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivoChapter 9: Analysis of small RNA profiles during development.Chapter 10: Dissecting MicroRNA-mediated Gene Regulation and Function in T Cell DevelopmentSECTION III. MiRNAs and DiseaseChapter 11: Investigation of microRNAs alterations in leukemias and lymphomasChapter 12: Discovery of pathogen-regulated small RNAs in plantsChapter 13: Protocols for expression and functional analysis of viral microRNAs