Microbes as Tools for Cell Biology
- David Russell, Washington University School of Medicine, St. Louis, Missouri, U.S.A.
- Leslie Wilson, University of California, Santa Barbara, U.S.A.
- Paul Matsudaira, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.
Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents.View full description
Researchers, students, and technicians in cell and developmental biology, microbiology, parasitology, immunology, biochemistry, pathology, genetics, and molecular biology.
Table of ContentsD.G. Russell, Preface.S. Falkow, Introduction.D.G. Russell, Obtaining and Maintaining Microbial Pathogens.Manipulation of Pathogens:W. Trager, Cultivation of Malaria Parasites.D.S. Roos, R.G.K. Donald, N.S. Morrissette, and A.L.C. Moulton, Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii.J.H. LeBowitz, Transfection Experiments with Leishmania.R. Tsolis and F. Heffron, Mutagenesis and Variant Selection in Salmonella.N.D. Connell, Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection.Microbial Adherence and Invasion Assays:G.J. Bancroft, H.L. Collins, L.B. Sigola, and C.E. Cross, Modulation of Murine Macrophage Behavior in Vivo and in Vitro.E.J. Brown, In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal Transduction.P. Falk, T. Borén, D. Haslam, and M. Caparon, Bacterial Adhesion and Colonization Assays.I. Crandall and I.W. Sherman, Cytoadherence and the Plasmodium falciparum-Infected Erythrocyte.The Study of Intracellular Pathogenesis:M.J. Blackman, Purification of Plasmodium falciparum Merozoites for Analysis of the Processing of Merozoite Surface Protein-1.K. Haldar, H.G. Elmendorf, A. Das, W.L. Li, D.J.P. Ferguson, and B.C. Elford, In Vitro Secretory Assays with Erythrocyte-Free Malaria Parasites.K.H. Berger and R.R. Isberg, Intracellular Survival by Legionella.P. Chakraborty, S. Sturgill-Koszycki, and D.G. Russell, Isolation and Characterization of Pathogen-Containing Phagosomes.D.G. Russell, Immunoelectron Microscopy of Endosomal Trafficking in Macrophages Infected with Microbial Pathogens.P.H. Schlesinger, Measuring the pH of Pathogen-Containing Phagosomes.C.V. Harding, Techniques for Studying Phagocytic Processing of Bacteria for Class I or II MHC-Restricted Antigen Recognition by T Lymphocytes.Chapter References.Subject Index.Volumes in Series.