Micro and Nanofabrication Using Self-Assembled Biological Nanostructures

Edited by

  • Jaime Castillo-León, Senior researcher, DTU Nanotech, Technical University of Denmark
  • Winnie Svendsen, Associate Professor, DTU Nanotech, Technical University of Denmark

Self-assembled nanostructures based on peptides and proteins have been investigated and presented as biomaterials with an impressive potential for a broad range of applications such as microfabrication, biosensing platforms, drug delivery systems, bioelectronics and tissue reparation. Through self-assembly peptides can give rise to a range of well-defined nanostructures such as nanotubes, nanofibers, nanoparticles, nanotapes, gels and nanorods. However, there are challenges when trying to integrate these biological nanostructures in the development of sensing devices or drug-delivery systems - challenges such as controlling the size during synthesis, the stability in liquid environments and manipulation.

In "Micro and Nanofabrication Using Self-assembled Biological Nanostructures" the options and challenges when using self-assembled peptide nanostructures in micro and nanofabrication are discussed. The publication covers different ways to manipulate, deposit and immobilize on specific locations these biological nanostructures in order to use them in the fabrication of new structures or as part of biosensing platforms. Examples where researchers used biological nanostructures for those types of applications are provided. Finally, future applications are discussed as well as parameters to accelerate and expand the use of these biological building blocks in nano- and micro-fabrication processes by taking advantage of their impressive properties such as low-cost and short synthesis time.

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Engineers and scientist working with biological materials or in biomedical or chemical engineering or in biochemistry; scientists doing research in the areas of biomaterials, cell handling, bionanotechnology, drug delivery, tissue engineering, regenerative medicine; nanotechnology students


Book information

  • Published: September 2014
  • ISBN: 978-0-323-29642-7

Table of Contents

Chapter 1 Self-Assembled Biological Nanofibers for Biosensor Applications
Luigi Sasso, Juliet A. Gerrard
1.1 Introduction
1.2 Types of Self-Assembled Biological Nanofibers
1.3 Practical Laboratory Considerations
1.4 Functionalization Approaches
1.5 Common Challenges in Biosensor Platforms
1.6 Conclusions
Chapter 2 Fabrication of Nanostructures Using Self-Assembled Peptides as Templates: The Diphenylalanine Case
Jaime Castillo-León
2.1 Introduction
2.2 Diphenylalanine Peptide
2.3 Fabrication of New Nanostructures Using Diphenylalanine Nanostructures as a Template
2.4 Conclusions
Chapter 3 Self-Assembled Peptide Nanostructures for the Fabrication of Cell Scaffolds
Rui Li, Alexandra Rodriguez, David R. Nisbet, Colin J. Barrow, Richard J. Williams
3.1 Introduction
3.2 Classes of Self-Assembled Peptide Scaffolds
3.3 Natural Self-Assembling Peptide Systems
3.4 Semisynthetic Self-Assembling Peptide Systems
3.5 Fabrication and Control of Mechanical Properties of Peptide Scaffolds
3.6 The In Vitro and In Vivo Applications of Self-Assembly Peptide Scaffolds
3.7 Biofunctionalization of Peptide Hydrogels
3.8 SAP Scaffolds as a Support for 3D Cell Culture
3.9 Utilizing Self-Assembly Peptide Scaffold as Cell Therapy In Vivo
3.10 Future Perspectives
Chapter 4 Self-Assembled Peptide Nanostructures for Regenerative Medicine and Biology
Ming Ni, Charlotte A.E. Hauser
4.1 Introduction
4.2 Peptide Building Blocks Constructing Nanostructures
4.3 Cell Adhesion
4.4 Tissue Engineering
4.5 Peptide Hydrogels as Vehicles for Controlled Drug Delivery
4.6 Peptide Therapeutics
4.7 Conclusion
Chapter 5 Fabrication of Drug Delivery Systems Using Self-Assembled Peptide Nanostructures
Daniel Keith, Honggang Cui
5.1 Introduction
5.2 Drug Delivery System Design
5.3 Peptide-Based Nanostructures as Drug Carriers
5.4 Self-Assembling Drug Amphiphiles as Novel Delivery Systems
5.5 Conclusion