Methods of Adipose Tissue Biology Part B book cover

Methods of Adipose Tissue Biology Part B

Methods of Adipose Tissue Biology

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.

Audience

Investigators and students whose research interests include the development, physiology, or pathophysiology of adipose tissues.

Included in series
Methods in Enzymology

Hardbound, 344 Pages

Published: February 2014

Imprint: Academic Press

ISBN: 978-0-12-800280-3

Contents

  • 1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
    Barbara Gawronska-Kozak
    2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover
    Tae-Hwa Chun and Mayumi Inoue
    3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
    Youn-Kyoung Lee and Chad A. Cowan
    4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
    Mi-Jeong Lee and Susan K. Fried
    5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation
    Ammar T. Qureshi, Cong Chen, Forum Shah, Caasy Thomas-Porch, Jeffrey M. Gimble and Daniel J. Hayes
    6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry
    Rodney C. Baker, Yana Nikitina and Angela R. Subauste
    7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
    Elena Dubikovskaya, Rostislav Chudnovskiy, Grigory Karateev, Hyo Min Park and Andreas Stahl
    8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity
    Jaeseok Han and Randal J. Kaufman
    9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes
    Andrea Galmozzi, Eduardo Dominguez, Benjamin F. Cravatt and Enrique Saez
    10. Measurement of Lipolysis
    Martina Schweiger, Thomas O. Eichmann, Ulrike Taschler, Robert Zimmermann, Rudolf Zechner and Achim Lass
    11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics
    Colleen E. Dugan and Robert T. Kennedy
    12. Methods for Performing Lipidomics in White Adipose Tissue
    Lee D. Roberts, James A. West, Antonio Vidal-Puig and Julian L. Griffin
    13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time
    Anne Bugge, Lea Dib and Sheila Collins
    14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes
    Qinghui Xu, Wendy S. Hahn and David A. Bernlohr
    15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis
    Emilio P. Mottillo, George M. Paul, Hsiao-Ping H. Moore and James G. Granneman
    16. Evaluation of Protein Phosphorylation during Adipogenesis
    Xi Li, Rong Zeng and Qi-Qun Tang

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