Methods in Methane Metabolism, Part A


Edited By

  • Amy Rosenzweig
  • Stephen Ragsdale

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.
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Researchers and the wider scientific community


Book information

  • Published: March 2011
  • ISBN: 978-0-12-385112-3

Table of Contents

  1. Techniques for Cultivating Methanogens
  2. Ralph S. Wolfe

  3. Genetic Methods for Methanosarcina species
  4. Nicole Buan, Gargi Kulkarni and William Metcalf

  5. Genetic Systems for Hydrogenotrophic Methanogens
  6. Felipe Sarmiento B, John A. Leigh and William B. Whitman

  7. Molecular tools for investigating ANME community structure and function
  8. Steven J. Hallam, Antoine Pagé, Lea Constan, Young C. Song, Angela Norbeck, Heather Mottaz, and Ljiljana Pasa-Tolic

  9. Studying gene regulation in methanogenic archaea
  10. Michael Rother, Christian Sattler and Tilmann Stock

  11. Growth of Methanogens Under Defined Hydrogen Conditions
  12. John A. Leigh

  13. Preparation of [Fe]-hydrogenase from methanogenic archaea
  14. Seigo Shima, Michael Schick and Haruka Tamura

  15. Assay of methylotrophic methyltransferases from methanogenic archaea
  16. Donald J. Ferguson, David G. Longstaff and Joseph A. Krzycki

  17. Methyl-Coenzyme M Reductase from Methanothermobacter marburgensis
  18. Evert C. Duin, Divya Prakash, Charlene Brungess

  19. Methods for Analysis of Acetyl-CoA Synthase: Applications to bacterial and archaeal systems
  20. David A. Grahame

  21. Acetate Kinase and Phosphotransacetylase
  22. James G. Ferry

  23. Sodium ion translocation and ATP synthesis in methanogens
  24. Katharina Schlegel and Volker Müller

  25. Proton translocation in methanogens
  26. Cornelia Welte and Uwe Deppenmeier

  27. Measuring isotope fractionation by autotrophic microorganisms and enzymes
  28. Kathleen M. Scott, Gordon Fox and Peter R. Girguis

  29. 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis
  30. David E. Graham

  31. Biomethanation and its potential

Irini Angelidaki, Fons Stams and Dimitar Karakashev