Methods for analysis of Golgi complex function book cover

Methods for analysis of Golgi complex function

Methods in Cell Biology

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.


Researchers and students in cell, molecular and developmental biology

Included in series
Methods in Cell Biology

Hardbound, 498 Pages

Published: November 2013

Imprint: Academic Press

ISBN: 978-0-12-417164-0


  • Model systems and functional studies
    1.Analysis of Golgi Complex Functions: In vitro Reconstitution Systems
      Frank Adolf and Felix T. Wieland
    2.RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture
      Victoria J. Miller, Caroline M. McKinnon, Harry Mellor, and David J. Stephens
    3.Trafficking along the Secretory Pathway in Drosophila Cell Line and Tissues:  A Light and Electron Microscopy Approach
      Margarita Zacharogianni and Catherine Rabouille
    4.Studying Membrane Trafficking in the Worm C. elegans by RNA Interference
      Zita Balklava and Elizabeth Sztul
    5.Endomembrane and Golgi Traffic in Plant Cells
      Giovanni Stefano, Anne Osterrieder, Chris Hawes, and Federica Brandizzi
    6.Biochemical Analysis of Secretory Trafficking in Mammalian Cells
      Peristera Roboti, Tomasz M. Witkos, and Martin Lowe
    7.Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems
      Alejo E. Rodríguez-Fraticelli and Fernando Martín-Belmonte
    8.Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells
      Lucy Pigati, Richard S. Kang, and Heike Fölsch
    9.Snap-Tagging the Retrograde Route
      Ludger Johannes and Massiullah Shafaq-Zadah
    10.Assessing ER and Golgi N-Glycosylation Process using Metabolic Labeling in Mammalian Cultured Cells
       Romain Péanne, Jorick Vanbeselaere, Dorothée Vicogne, Anne-Marie Mir, Christophe Biot, Gert Matthijs, Yann Guérardel, and François Foulquier

    Imaging based approaches
    11.Fluorescence based Analysis of Trafficking in Mammalian Cells
       Gaelle Boncompain and Franck Perez
    12.Photobleaching Methods to study Golgi Complex Dynamics in Living Cells
       Erik Lee Snapp
    13.Photohighlighting approaches to access membrane dynamics of the Golgi apparatus
       Jennifer Lippincott-Schwartz and Prabuddha Sengupta
    14.Live Cell Visualization of Golgi Membrane Dynamics by Super-Resolution Confocal Live Imaging Microscopy (SCLIM)
       Kazuo Kurokawa, Midori Ishii, Yasuyuki Suda, Akira Ichihara, and Akihiko Nakano
    15.Analysis of Golgi Complex Function using Correlative Light-Electron Microscopy (CLEM)
       Elena V. Polishchuk and Roman S. Polishchuk
    16.Viewing Golgi Structure and Function from a different perspective - Insights from Electron Tomography
       Margit Pavelka and Brad J. Marsh
    17.High Content Screening and Analysis of the Golgi Complex
       George Galea and Jeremy C. Simpson

    Emerging studies [New Frontiers]
    18.(Re)Modeling the Golgi
       Pierre Sens and Madan Rao
    19.Golgi Depletion from Living Cells with Laser Nanosurgery
       Paolo Ronchi and Rainer Pepperkok
    20.Studying Intracellular Trafficking Pathways with Probabilistic Density Maps
       Kristine Schauer, Tarn Duong, Carina S. Gomes-Santos, and Bruno Goud
    21.Methods to Study Signaling at The Golgi Apparatus
       Veronika Reitere, Francesco Baschieri, Valentina Millarte, and Hesso Farhan
    22.Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking
       Jorge Cancino, Anita Capalbo, and Alberto Luini
    23.Signaling at the Golgi during Mitosis
       Antonino Colanzi and Christine Sütterlin
    24.Ice Recovery Assay for Detection of Golgi-Derived Microtubules
       Ashley D. Grimaldi, Maria Fomicheva, and Irina Kaverina


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