Metallobiochemistry, Part A book cover

Metallobiochemistry, Part A

Volume 158: Metabiochemistry Part A

@from:From the PrefaceAlthough it has been suspected since ancient times that metals participate in biological processes, details of the manner in which they might exert a function have until recently remained unknown. It is now appreciated that metals play roles in catalysis, hormone action, gene and other regulatory functions, structural stabilization of macromolecules, muscle contraction, nerve conduction, and transport. "Metallobiochemistry" is a field which has grown and matured rapidly over the past decade. Metalloenzymes have been the central investigating targets. This volume provides both the emerging and practicing metallobiochemist with the necessary tools to answer what should be the number one and two questions: Is this enzyme a metalloenzyme? What metal is it?

Audience
Biochemists, biophysicists, analytical chemists, physical chemists, and clinical chemists

Included in series
Methods in Enzymology

Hardbound, 464 Pages

Published: April 1988

Imprint: Academic Press

ISBN: 978-0-12-182059-6

Reviews

  • "The Methods in Enzymology series represents the gold-standard."
    Praise for the Series, --NEUROSCIENCE


    "Incomparably useful."
    --ANALYTICAL BIOCHEMISTRY
    "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
    --BIO/TECHNOLOGY
    "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
    --CHEMISTRY IN INDUSTRY
    "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
    --AMERICAN SOCIETY OF MICROBIOLOGY NEWS
    "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
    --ENZYMOLOGIA
    "A series that has established itself as a definitive reference for biochemists."
    --JOURNAL OF CHROMATOGRAPHY

Contents

  • Sample Preparation:J.F. Riordan and B.L. Vallee, Preparation of Metal-Free Water.B. Holmquist, Elimination of Adventitious Metals.D.S. Auld, Metal-Free Dialysis Tubing.M.T. Martin, Metal-Free Chromatographic Media.F.W. Wagner, Preparation of Metal-Free Enzymes.J.O. Baker, Metal-Buffered Systems.C. Veillon, Standards for Metal Analysis.D.S. Auld, Methods for Metal Substitution.W. Maret and M. Zeppezauer, Preparation of Metal-Hybrid Enzymes.H.E. Van Wart, Introduction of Exchange-Inert Metal Ions into Enzymes.D.S. Auld, Use of Chelating Agents to Inhibit Enzymes.Analytical Techniques.W. Slavin, Atomic Absorption Spectrometry.J.M. Harnley and D.L. Garland, Multielement Atomic Absorption Methods of Analysis.S. Chandra and G.H. Morrison, Ion Microscopy in Biology and Medicine.T.H. Risby, Flame Atomic Emission Spectrometry.K.A. Wolnik, Inductively Coupled Plasma-Emission Spectrometry.J.A. Olivares, Inductively Coupled Plasma-Mass Spectrometry.R.G. Michel, Atomic Fluorescence Spectrometry.J. Osteryoung, Electrochemical Methods of Analysis.J. Versieck, Neutron Activation Analysis.Analysis of Metals.J. Savory, S. Brown, R.L. Bertholf, N. Mendoza, and M.R. Wills, Aluminum.G.N. Bowers, Jr., and T.C. Rains, Measurement of Total Calcium in Biological Fluids: Flame Atomic Absorption Spectrometry.S.F. Sena and G.N. Bowers, Jr. , Measurement of Ionized Calcium in Biological Fluids: Ion-Selective Electrode Method.C. Veillon, Chromium. R. Shapiro and M.T. Martin, Determination of Cobalt by Atomic Absorption Spectrometry.M.A. Evenson, Measurement of Copper in Biological Samples by Flame or Electrothermal Atomic Absorption Spectrometry.W.W. Fish, Rapid Colorimetric Micromethod for the Quantitation of Complexed Iron in Biological Samples.M.T. Martin and R. Shapiro, Atomic Absorption Spectrometry of Magnesium.J.L. Johnson, Molybdenum.F.W. Sunderman, Jr., S.M. Hopfer, and M.C. Crisostomo, Nickel Analysis by Electrothermal Atomic Absorption Spectrometry.S.A. Lewis, Determination of Selenium in Biological Matrices.D.M. Martin and N.D. Chasteen, Vanadium.K.H. Falchuk, K.L. Hilt, and B.L. Vallee, Determination of Zinc in Biological Samples by Atomic Absorption Spectrometry.Author Index.Subject Index.

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