Metabolic Engineering

Principles and Methodologies


  • George Stephanopoulos, Massachusetts Institute of Technology, Cambridge, U.S.A.
  • Aristos Aristidou, VTT Biotechnology and Food Research
  • Jens Nielsen, Tech University of Denmark

Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes.Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems.
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Microbial physiologists, chemical engineers, biochemists, and biotechnologists who work on the application of molecular techniques to the alteration of cell cultures and microorganisms. Advanced graduate-level courses in biochemical engineering.


Book information

  • Published: October 1998
  • ISBN: 978-0-12-666260-3

Table of Contents

The Essence of Metabolic Engineering. Review of Cellular Metabolism. Comprehensive Models for Cellular Reactions. Material Balances and Data Consistency. Regulation of Metabolic Pathways. Examples of Pathway Manipulations: Metabolic Engineering in Practice. Metabolic Pathway Synthesis. Metabolic Flux Analysis. Methods for the Experimental Determination of Metabolic Fluxes by Isotope Labeling. Applications of Metabolic Flux Analysis. Metabolic Control Analysis. Analysis of Structure of Metabolic Networks. Flux Analysis of Metabolic Networks. Thermodynamics of Cellular Processes. Glossary. Subject Index.