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Metabolic Analysis Using Stable Isotopes
1st Edition, Volume 561 - September 8, 2015
Editor: Christian Metallo
Language: English
Hardback ISBN:9780128022931
9 7 8 - 0 - 1 2 - 8 0 2 2 9 3 - 1
eBook ISBN:9780128025345
9 7 8 - 0 - 1 2 - 8 0 2 5 3 4 - 5
Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the f…Read more
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, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes.
Continues the legacy of this premier serial with quality chapters on metabolic analysis using stable isotopes
Represents the newest volume in Methods in Enzymology, providing a premier serial with quality chapters authored by leaders in the field
Ideal reference for those interested in the study of metabolism, metabolic tracing, isotopic labeling, and lipogenesis
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
Dedication
Preface
Chapter One: Studies of Metabolism Using 13C MRS of Hyperpolarized Probes
Abstract
1 Introduction
2 13C MRS of DNP Probes: Current Approaches to Studying Metabolism
3 Requirements of 13C DNP Probes
4 Conducting Hyperpolarized Experiments
5 Acquisition Strategies for Hyperpolarized MR Spectroscopy and Imaging
6 Analysis of Hyperpolarized MR Data
7 Summary and Conclusion
Chapter Two: Hyperpolarized 13C Magnetic Resonance and Its Use in Metabolic Assessment of Cultured Cells and Perfused Organs
Abstract
1 Introduction: Importance of Developing Methods to Observe Metabolic Flux in Disease States
2 Hyperpolarization Methods and Sample Preparation
3 Dynamic Assessment of Metabolism in Cells
4 Dynamic Assessment of Metabolism in Perfused Organs
5 Challenges and Future Directions
6 Conclusion
Acknowledgments
Chapter Three: Metabolic Tracing Using Stable Isotope-Labeled Substrates and Mass Spectrometry in the Perfused Mouse Heart
Abstract
1 Introduction
2 Study Model: Mouse Heart Perfusion Ex Vivo in the Working Mode with Semi-Recirculating Buffer
3 Metabolic Flux Measurements in the Perfused Mouse Heart Using 13C-Labeled Substrates and Gas Chromatography-Mass Spectrometry
4 Practical Considerations and Future Challenges
Acknowledgments
Chapter Four: Probing Metabolism in the Intact Retina Using Stable Isotope Tracers
Abstract
1 Introduction
2 Methods
3 Applications
4 Summary
Acknowledgments
Chapter Five: Analysis of Cell Metabolism Using LC-MS and Isotope Tracers
Abstract
1 Introduction
2 Methods for Sample Extraction
3 LC-MS Methods
4 LC-MS Data Analysis
5 Use of Stable Isotope Tracers
6 Normalization
7 Quantification of Metabolites in Cells and Medium
8 Stable Isotope Tracing with Fumarate Hydratase-Deficient Cell Model
9 Future
Chapter Six: Analysis of Fatty Acid Metabolism Using Stable Isotope Tracers and Mass Spectrometry
Abstract
1 Introduction
2 Labeling and Extraction of Fatty Acids in Cultured Cells
3 Mass Spectrometry Analysis of Fatty Acid Labeling
4 Fatty Acid-Labeling Data Analysis and Interpretation
5 Summary and Perspective
Chapter Seven: Dynamic Proteomics: In Vivo Proteome-Wide Measurement of Protein Kinetics Using Metabolic Labeling
Abstract
1 Introduction
2 Fundamentals: Stable Isotope-Labeling Approaches for Measuring Protein Synthesis and Other Polymerization Biosynthesis Rates
3 Application of Combinatorial Analysis (MIDA) Approaches to Protein Dynamics